Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1975 May;28(5):909–924.

Cellular and humoral immune responses in mice. II. Effect of intraperitoneal or subcutaneous injection of carrier of anti-hapten antibody and delayed hypersensitivity responses.

S I Tamura, Y Egashira
PMCID: PMC1445915  PMID: 1169220

Abstract

The subcutaneous (s.c.) injection of sheep red blood cells (SRBC) without adjuvant into mice preferentially induced delayed hypersensitivity (DH) reaction, as measured by footpad swelling, while intraperitoneal (i.p.) measured by the haemagglutinin test. Under these conditions, the properties of the helper activities of thymus-derived (T) cells for humoral responses were examined, in association with the features of the DH response, by measuring the anti-hapten and anticarrier antibody responses after a booster injection of trinitrophenylated (TNP) SRBC and by changing the combination of doses and injections routes of the carrier and the hapten-carrier conjugates. When mice were presensitized with i.p. injections of SRBC and boosted with i.p. injections of TNP-SRBC, the anti-TNP antibody production was maximally enhanced by presensitization with a low dose of SRBC, and gradually abolished with higher doses of SRBC for pre-sensitization. In the latter case, anti-SRBC antibody production was increases with increasing doses of SRBC..

Full text

PDF
909

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Claman H. N., Chaperon E. A., Triplett R. F. Thymus-marrow cell combinations. Synergism in antibody production. Proc Soc Exp Biol Med. 1966 Aug-Sep;122(4):1167–1171. doi: 10.3181/00379727-122-31353. [DOI] [PubMed] [Google Scholar]
  2. Eidinger D., Ackerman A. A cellular deficit in the reconstitutive capacity of immune populations of lymphoid cells demonstrable in studies of delayed hypersensitivity in mice. Evidence for thymus-bone marrow cell synergism. J Exp Med. 1971 May 1;133(5):1061–1073. doi: 10.1084/jem.133.5.1061. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Kappler J. W., Hoffmann M., Dutton R. W. Regulation of the immune response. I. Differential effect of passively administered antibody on the thymus-derived and bone marrow-derived lymphocytes. J Exp Med. 1971 Sep 1;134(3 Pt 1):577–587. doi: 10.1084/jem.134.3.577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kettman J., Dutton R. W. An in vitro primary immune response to 2,4,6-trinitrophenyl substituted erythrocytes: response against carrier and hapten. J Immunol. 1970 Jun;104(6):1558–1561. [PubMed] [Google Scholar]
  5. Kettman J., Dutton R. W. Radioresistance of the enhancing effect of cells from carrier-immunized mice in an in vitro primary immune response. Proc Natl Acad Sci U S A. 1971 Apr;68(4):699–703. doi: 10.1073/pnas.68.4.699. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kishimoto T., Ishizaka K. Regulation of antibody response in vitro. V. Effect of carrier-specific Helper cells on generation of hapten-specific memory cells of different immunoglobulin classes. J Immunol. 1973 Jul;111(1):1–9. [PubMed] [Google Scholar]
  7. Mitchell G. F., Miller J. F. Cell to cell interaction in the immune response. II. The source of hemolysin-forming cells in irradiated mice given bone marrow and thymus or thoracic duct lymphocytes. J Exp Med. 1968 Oct 1;128(4):821–837. doi: 10.1084/jem.128.4.821. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Parish C. R. Immune response to chemically modified flagellin. II. Evidence for a fundamental relationship between humoral and cell-mediated immunity. J Exp Med. 1971 Jul 1;134(1):21–47. doi: 10.1084/jem.134.1.21. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Parish C. R. The relationship between humoral and cell-mediated immunity. Transplant Rev. 1972;13:35–66. doi: 10.1111/j.1600-065x.1972.tb00059.x. [DOI] [PubMed] [Google Scholar]
  10. Rabellino E., Colon S., Grey H. M., Unanue E. R. Immunoglobulins on the surface of lymphocytes. I. Distribution and quantitation. J Exp Med. 1971 Jan 1;133(1):156–167. doi: 10.1084/jem.133.1.156. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Raff M. C., Owen J. J. Thymus-derived lymphocytes: their distribution and role in the development of peripheral lymphoid tissues of the mouse. Eur J Immunol. 1971 Jan;1(1):27–30. doi: 10.1002/eji.1830010105. [DOI] [PubMed] [Google Scholar]
  12. Raff M. C. Role of thymus-derived lymphocytes in the secondary humoral immune response in mice. Nature. 1970 Jun 27;226(5252):1257–1258. doi: 10.1038/2261257a0. [DOI] [PubMed] [Google Scholar]
  13. Rajewsky K., Schirrmacher V., Nase S., Jerne N. K. The requirement of more than one antigenic determinant for immunogenicity. J Exp Med. 1969 Jun 1;129(6):1131–1143. doi: 10.1084/jem.129.6.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rittenberg M. B., Pratt K. L. Antitrinitrophenyl (TNP) plaque assay. Primary response of Balb/c mice to soluble and particulate immunogen. Proc Soc Exp Biol Med. 1969 Nov;132(2):575–581. doi: 10.3181/00379727-132-34264. [DOI] [PubMed] [Google Scholar]
  15. Schirrmacher V., Rajewsky K. Determination of antibody class in a system of cooperating antigenic determinants. J Exp Med. 1970 Nov;132(5):1019–1034. doi: 10.1084/jem.132.5.1019. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Segal S., Cohen I. R., Feldman M. Thymus-derived lymphocytes: humoral and cellular reactions distinguished by hydrocortisone. Science. 1972 Mar 10;175(4026):1126–1128. doi: 10.1126/science.175.4026.1126. [DOI] [PubMed] [Google Scholar]
  17. Takatsu K., Hamaoka T., Kitagawa M. Antibody production in mice. VI. Effect of anti-carrier antibody on cellular co-operation in the primary anti-hapten antibody response. Immunology. 1974 Feb;26(2):233–246. [PMC free article] [PubMed] [Google Scholar]
  18. Tamura S., Kurata T., Sugimoto M., Egashira Y. Cellular and humoral immune responses in mice. I. Development of delayed-type footpad swelling against sheep erythrocytes and its suppression by intraperitoneal administration of the antigen. Jpn J Med Sci Biol. 1973 Aug;26(4):161–168. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES