Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1975 May;28(5):847–854.

Age-related decline in the antibody response to E. coli lipopolysaccharide in New Zealand Black mice.

M J Blankwater, L A Levert, W Hijmans
PMCID: PMC1445920  PMID: 1093967

Abstract

A thymus-independent immune function in ageing NZB and BALB/c mice was compared by measuring the antibody response to E. coli lipopolysaccharide (LPS). Since it was found that 10-13 month-old NZB mice was particularly sensitive to LPS, this antigen had to be detoxified by alkali treatment. The anti-LPS splenic plaque-forming cell (PFC) response of NZB mice decreases with age and was lower than that of BALB/c mice in all age groups studied. The response of 4- and 10-month-old NZB mice showed an irregular time course and a number of mice showed no response. The present results indicate that, besides an impairment of T-cell functions, an impairment on the B-cell level must also be considered in ageing NZB mice.

Full text

PDF
847

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson B., Blomgren H. Evidence for thymus-independent humoral antibody production in mice against polyvinylpyrrolidone and E. coli lipopolysaccharide. Cell Immunol. 1971 Oct;2(5):411–424. doi: 10.1016/0008-8749(71)90052-9. [DOI] [PubMed] [Google Scholar]
  2. Bach J. F., Dardenne M., Salomon J. C. Studies on thymus products. IV. Absence of serum 'thymic activity' in adult NZB and (NZB x NZW) F1 mice. Clin Exp Immunol. 1973 Jun;14(2):247–256. [PMC free article] [PubMed] [Google Scholar]
  3. Baker P. J., Barth R. F., Stashak P. W., Amsbaugh D. F. Enhancement of the antibody response to type 3 pneumococcal polysaccharide in mice treated with antilymphocyte serum. J Immunol. 1970 May;104(5):1313–1315. [PubMed] [Google Scholar]
  4. Barthold D. R., Kysela S., Steinberg A. D. Decline in suppressor T cell function with age in female NZB mice. J Immunol. 1974 Jan;112(1):9–16. [PubMed] [Google Scholar]
  5. Baum J. Increased 7S antibody response to sheep erythrocytes in the 2-month-old NZB mouse. Clin Exp Immunol. 1969 Sep;5(3):251–263. [PMC free article] [PubMed] [Google Scholar]
  6. Britton S. Regulation of antibody synthesis against Escherichia coli endotoxin. II. Specificity, dose requirements and duration of paralysis induced in adult mice. Immunology. 1969 Apr;16(4):513–526. [PMC free article] [PubMed] [Google Scholar]
  7. Cantor H., Asofsky R., Talal N. Synergy among lymphoid cells mediating the graft-versus-host response. I. Synergy in graft-versus-host reactions produced by cells from NZB-Bl mice. J Exp Med. 1970 Feb;131(2):223–234. doi: 10.1084/jem.131.2.223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Cerottini J. C., Lambert P. H., Dixon F. J. Comparison of the immune responsiveness of NZB and NZB X NZW F1 hybrid mice with that of other strains of mice. J Exp Med. 1969 Nov 1;130(5):1093–1105. doi: 10.1084/jem.130.5.1093. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Cohen A., Schlesinger M. Absorption of guinea pig serum with agar. A method for elimination of itscytotoxicity for murine thymus cells. Transplantation. 1970 Jul;10(1):130–132. doi: 10.1097/00007890-197007000-00027. [DOI] [PubMed] [Google Scholar]
  10. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  11. De Jesus D. G., Holborow E. J., Brown J. C. A defect of B-lymphocyte transport of aggregated HGG into germinal centres in NZB and NZB x NZW F 1 hybrid mice. Clin Exp Immunol. 1972 Aug;11(4):507–522. [PMC free article] [PubMed] [Google Scholar]
  12. Elkerbout E. A., Hijmans W. Relative avidity of antibodies towards sheep red blood cells in New Zealand Black mice. Immunology. 1974 May;26(5):901–907. [PMC free article] [PubMed] [Google Scholar]
  13. Elkerbout E. A., Hijmans W. The long-term antibody response of New Zealand Black mice to sheep red blood cells. Immunology. 1974 May;26(5):893–900. [PMC free article] [PubMed] [Google Scholar]
  14. Howie J. B., Helyer B. J. The immunology and pathology of NZB mice. Adv Immunol. 1968;9:215–266. doi: 10.1016/s0065-2776(08)60444-7. [DOI] [PubMed] [Google Scholar]
  15. Jacobs M. E., Gordon J. K., Talal N. Inability of the NZB-NZW F 1 thymus to transfer cyclophosphamide-induced tolerance to sheep erythrocytes. J Immunol. 1971 Aug;107(2):359–364. [PubMed] [Google Scholar]
  16. Leventhal B. G., Talal N. Response of NZB and NZB-NZW spleen cells to mitogenic agents. J Immunol. 1970 Apr;104(4):918–923. [PubMed] [Google Scholar]
  17. Mellors R. C. Autoimmune and immunoproliferative diseases of NZB/Bl mice and hybrids. Int Rev Exp Pathol. 1966;5:217–252. [PubMed] [Google Scholar]
  18. Morton J. I., Siegel B. V. Response of NZB mice to foreign antigen and development of autoimmune disease. J Reticuloendothel Soc. 1969 Feb;6(1):78–93. [PubMed] [Google Scholar]
  19. Möller G., Michael G. Frequency of antigen-sensitive cells to thymus-independent antigens. Cell Immunol. 1971 Aug;2(4):309–316. doi: 10.1016/0008-8749(71)90065-7. [DOI] [PubMed] [Google Scholar]
  20. NORINS L. C., HOLMES M. C. ANTINUCLEAR FACTOR IN MICE. J Immunol. 1964 Jul;93:148–154. [PubMed] [Google Scholar]
  21. Petty R. E., Steward M. W. Relative affinity of anti-protein antibodies in New Zealand mice. Clin Exp Immunol. 1972 Nov;12(3):343–350. [PMC free article] [PubMed] [Google Scholar]
  22. Playfair J. H. Strain differences in the immune responses of mice. 3. A raised tolerance threshold in NZB thymus cells. Immunology. 1971 Dec;21(6):1037–1043. [PMC free article] [PubMed] [Google Scholar]
  23. Purves E. C., Playfair J. H. Normal tolerance characteristics of the antibody-forming cell precursors of the NZB mouse. Clin Exp Immunol. 1973 Sep;15(1):113–122. [PMC free article] [PubMed] [Google Scholar]
  24. Robson H. G., Vas S. I. Resistance of inbred mice to Salmonella typhimurium. J Infect Dis. 1972 Oct;126(4):378–386. doi: 10.1093/infdis/126.4.378. [DOI] [PubMed] [Google Scholar]
  25. Rodey G. E., Good R. A., Yunis E. J. Progressive loss in vitro of cellular immunity with ageing in strains of mice susceptible to autoimmune disease. Clin Exp Immunol. 1971 Sep;9(3):305–311. [PMC free article] [PubMed] [Google Scholar]
  26. Rotter V., Trainin N. Thymus cell population exerting a regulatory function in the immune response of mice to polyvinyl pyrrolidone. Cell Immunol. 1974 Jul;13(1):76–86. doi: 10.1016/0008-8749(74)90228-7. [DOI] [PubMed] [Google Scholar]
  27. Shirai T., Mellors R. C. Natural thymocytotoxic autoantibody and reactive antigen in New Zealand black and other mice. Proc Natl Acad Sci U S A. 1971 Jul;68(7):1412–1415. doi: 10.1073/pnas.68.7.1412. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Staples P. J., Steinberg A. D., Talal N. Induction of immunologic tolerance in older New Zealand mice repopulated with young spleen, bone marrow, or thymus. J Exp Med. 1970 Jun 1;131(6):1223–1238. doi: 10.1084/jem.131.6.1223. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Staples P. J., Talal N. Relative inability to induce tolerance in adult NZB and NZB-NZW F1 mice. J Exp Med. 1969 Jan 1;129(1):123–139. doi: 10.1084/jem.129.1.123. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Stutman O. Lymphocyte subpopulations in NZB mice: deficit of thymus-dependent lymphocytes. J Immunol. 1972 Sep;109(3):602–611. [PubMed] [Google Scholar]
  31. Stutman O., Yunis E. J., Good R. A. Deficient immunologic functions of NZB mice. Proc Soc Exp Biol Med. 1968 Apr;127(4):1204–1207. doi: 10.3181/00379727-127-32910. [DOI] [PubMed] [Google Scholar]
  32. Teague P. O., Yunis E. J., Rodey G., Fish A. J., Stutman O., Good R. A. Autoimmune phenomena and renal disease in mice. Role of thymectomy, aging, and involution of immunologic capacity. Lab Invest. 1970 Feb;22(2):121–130. [PubMed] [Google Scholar]
  33. Veit B. C., Michael J. G. The lack of thymic influence in regulating the immune response to Escherichia coli 0127 endotoxin. J Immunol. 1972 Sep;109(3):547–553. [PubMed] [Google Scholar]
  34. Weir D. M., McBride W., Naysmith J. D. Immune response to a soluble protein antigen in NZB mice. Nature. 1968 Sep 21;219(5160):1276–1277. doi: 10.1038/2191276a0. [DOI] [PubMed] [Google Scholar]
  35. Zaalberg O. B., van der Meul V. A., van Twisk M. J. Antibody production by isolated spleen cells: a study of the cluster and the plaque techniques. J Immunol. 1968 Feb;100(2):451–458. [PubMed] [Google Scholar]
  36. de Vries M. J., Hijmans W. A deficient development of the thymic epithelium and auto-immune disease in NZB mice. J Pathol Bacteriol. 1966 Apr;91(2):487–494. doi: 10.1002/path.1700910223. [DOI] [PubMed] [Google Scholar]
  37. de Vries M. J., Hijmans W. Pathological changes of thymic epithelial cells and autoimmune disease in NZB, NZW and (NZB x NZW)F1 mice. Immunology. 1967 Feb;12(2):179–196. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES