Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1975 Aug;29(2):353–364.

Evidence for a membrane carrier molecule common to embryonal and tumour-specific antigenic determinants expressed by a mouse transplantable tumour.

P M Comoglio, M Bertini, G Forni
PMCID: PMC1445940  PMID: 51002

Abstract

Rabbits were primed with membrane antigens solubilized from BALB/c embryo cells. After boosting with membrane antigens solubilized from a syngeneic transplantable adenocarcinoma, they developed a 'secondary' response against tumour-specific antigenic determinants. The antibodies against these determinants neither reacted with nor were absorbed by the antigens prepared from embryonal cells. However, the antigen displaying the tumour-specific determinants was bound by a reversed immunoadsorbent of insoluble anti-embryo antibodies. Indirect immunofluorescence experiments performed on adenocarcinoma cells in culture showed that, under conditions where redistribution of cell membrane components was induced, the anti-embryo antiserum aggregated the tumour-specific determinants. The purification of embryo and tumour-specific antigens achieved by affinity chromatography on insoluble antibody columns yielded three polypeptides of molecular weight close to 25,000, 20,000, and 10,000 Daltons respectively. It is suggested that the antigenic determinants responsible for tumour and embryo specificities in adenocarcinoma were located on the same molecule, or, more likely, on molecules which are closely associated in the plasma membrane and that do not dissociated in bile salts.

Full text

PDF
353

Images in this article

358FIG. 1
on p.358
359FIG. 3
on p.359
360FIG. 4
on p.360

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abelev G. I. Production of embryonal serum alpha-globulin by hepatomas: review of experimental and clinical data. Cancer Res. 1968 Jul;28(7):1344–1350. [PubMed] [Google Scholar]
  2. Axén R., Ernback S. Chemical fixation of enzymes to cyanogen halide activated polysaccharide carriers. Eur J Biochem. 1971 Feb 1;18(3):351–360. doi: 10.1111/j.1432-1033.1971.tb01250.x. [DOI] [PubMed] [Google Scholar]
  3. CLARK H. F., SHEPARD C. C. A DIALYSIS TECHNIQUE FOR PREPARING FLUORESCENT ANTIBODY. Virology. 1963 Aug;20:642–644. doi: 10.1016/0042-6822(63)90292-7. [DOI] [PubMed] [Google Scholar]
  4. Coggin J. H., Ambrose K. R., Anderson N. G. Fetal antigen capable of inducing transplantation immunity against SV40 hamster tumor cells. J Immunol. 1970 Aug;105(2):524–526. [PubMed] [Google Scholar]
  5. Comoglio P. M., Filogamo G. Plasma membrane fluidity and surface motility of mouse C-1300 neuroblastoma cells. J Cell Sci. 1973 Sep;13(2):415–420. doi: 10.1242/jcs.13.2.415. [DOI] [PubMed] [Google Scholar]
  6. Cresswell P., Turner M. J., Strominger J. L. Papain-solubilized HL-A antigens from cultured human lymphocytes contain two peptide fragments. Proc Natl Acad Sci U S A. 1973 May;70(5):1603–1607. doi: 10.1073/pnas.70.5.1603. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Fujimoto S., Chen C. H., Sabbadini E., Sehon A. H. Association of tumor and histocompatibility antigens in sera of lymphoma-bearing mice. J Immunol. 1973 Oct;111(4):1093–1100. [PubMed] [Google Scholar]
  8. Gilden R. V., Oroszlan S., Huebner R. J. Coexistence of intraspecies and interspecies specific antigenic determinants on the major structural polypeptide of mammalian C-type viruses. Nat New Biol. 1971 May 26;231(21):107–108. doi: 10.1038/newbio231107a0. [DOI] [PubMed] [Google Scholar]
  9. Gold P., Freedman S. O. Specific carcinoembryonic antigens of the human digestive system. J Exp Med. 1965 Sep 1;122(3):467–481. doi: 10.1084/jem.122.3.467. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Graham J. M., Sumner M. C., Curtis D. H., Pasternak C. A. Sequence of events in plasma membrane assembly during the cell cycle. Nature. 1973 Nov 30;246(5431):291–295. doi: 10.1038/246291a0. [DOI] [PubMed] [Google Scholar]
  11. KLEIN G., SJOGREN H. O., KLEIN E., HELLSTROM K. E. Demonstration of resistance against methylcholanthrene-induced sarcomas in the primary autochthonous host. Cancer Res. 1960 Dec;20:1561–1572. [PubMed] [Google Scholar]
  12. Katz D. H., Paul W. E., Goidl E. A., Benacerraf B. Carrier function in anti-hapten immune responses. I. Enhancement of primary and secondary anti-hapten antibody responses by carrier preimmunization. J Exp Med. 1970 Aug 1;132(2):261–282. doi: 10.1084/jem.132.2.261. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Paul W. E., Katz D. H., Goidl E. A., Benacerraf B. Carrier function in anti-hapten immune responses. II. Specific properties of carrier cells capable of enhancing anti-hapten antibody responses. J Exp Med. 1970 Aug 1;132(2):283–299. doi: 10.1084/jem.132.2.283. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pearson G., Freeman G. Evidence suggesting a relationship between polyoma virus-induced transplantation antigen and normal embryonic antigen. Cancer Res. 1968 Sep;28(9):1665–1673. [PubMed] [Google Scholar]
  15. Rajewsky K., Rottländer E., Peltre G., Müller B. The immune response to a hybrid protein molecule; specificity of secondary stimulation and of tolerance induction. J Exp Med. 1967 Oct 1;126(4):581–606. doi: 10.1084/jem.126.4.581. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Rajewsky K., Schirrmacher V., Nase S., Jerne N. K. The requirement of more than one antigenic determinant for immunogenicity. J Exp Med. 1969 Jun 1;129(6):1131–1143. doi: 10.1084/jem.129.6.1131. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Solheim B. G., Thorsby E. Beta-2-microglobulin. Part of the HL-A molecule in the cell membrane. Tissue Antigens. 1974;4(1):83–94. doi: 10.1111/j.1399-0039.1974.tb00229.x. [DOI] [PubMed] [Google Scholar]
  18. Stonehill E. H., Bendich A. Retrogenetic expression: the reappearance of embryonal antigens in cancer cells. Nature. 1970 Oct 24;228(5269):370–372. doi: 10.1038/228370a0. [DOI] [PubMed] [Google Scholar]
  19. Takahashi T., Old L. J., Hsu C. J., Boyse E. A. A new differentiation antigen of plasma cells. Eur J Immunol. 1971 Dec;1(6):478–482. doi: 10.1002/eji.1830010614. [DOI] [PubMed] [Google Scholar]
  20. Thomson D. M., Steele K., Alexander P. The presence of tumour-specific membrane antigen in the serum of rats with chemically induced sarcomata. Br J Cancer. 1973 Jan;27(1):27–34. doi: 10.1038/bjc.1973.4. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Ting R. C. Failure to induce transplantation resistance against polyoma tumour cells with syngeneic embryonic tissues. Nature. 1968 Mar 2;217(5131):858–859. doi: 10.1038/217858a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES