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. 1975 Nov;29(5):875–884.

In vitro proliferative response of BALB/c mouse spleen cells stimulated with trinitrophenylated syngeneic spleen cells.

H Tokuyama
PMCID: PMC1445988  PMID: 127744

Abstract

Spleen cells from normal BALB/c mice showed in vitro proliferative response against hapten-conjugated syngeneic spleen cells. Trinitrophenylated (TNP) spleen cells were prepared by treating normal spleen cells with sodium 2,4,6-trinitrobenzenesulphonate (TNBS). Four-day cultures of TNP-labelled spleen cells incorporated 2.5-7.4 times more [3H]thymidine than similar cultures of untreated spleen cells. An obviously positive mixed lymphocyte reaction (MLR) by normal spleen cells against mitomycin C (MC) treated TNP-labeled syngeneic spleen cells was observed after 4 days of culture. The MLR to TNP-labelled syngeneic cells was inhibited in the presence of epsilon-TNP-L-lysine by 23-37%. The spleen cells from the mice injected intraperitoneally with TNP-labelled syngeneic spleen cells showed a higher MLR against TNP-labelled spleen cells than normal spleen cells. The sensitized spleen cells also showed an increased response to MC-treated spleen cells. These results suggest that normal spleen cells include cells which can recognize the hapten and new antigenic determinants introduced into syngeneic spleen by chemical modification.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Asherson G. L., Zembala M. Suppression of contact sensitivity by T cells in the mouse. I. Demonstration that suppressor cells act on the effector stage of contact sensitivity; and their induction following in vitro exposure to antigen. Proc R Soc Lond B Biol Sci. 1974 Nov 5;187(1088):329–348. doi: 10.1098/rspb.1974.0078. [DOI] [PubMed] [Google Scholar]
  2. Geczy A. F., Baumgarten A. Lymphocyte transformation in contact sensitivity. Immunology. 1970 Aug;19(2):189–203. [PMC free article] [PubMed] [Google Scholar]
  3. Heber-Katz E., Click R. E. Immune responses in vitro. V. Role of mercaptoethanol in the mixed-leukocyte reaction. Cell Immunol. 1972 Nov;5(3):410–418. doi: 10.1016/0008-8749(72)90067-6. [DOI] [PubMed] [Google Scholar]
  4. Howe M. L., Goldstein A. L., Battisto J. R. Isogeneic lymphocyte interaction: recognition of self antigens by cells of the neonatal thymus. Proc Natl Acad Sci U S A. 1970 Oct;67(2):613–619. doi: 10.1073/pnas.67.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Miller A. E., Levis W. R. Lymphocyte transformation during dinitrochlorobenzene contact sensitization. An in vitro and in vivo evaluation of the primary immune response in man. J Clin Invest. 1973 Aug;52(8):1925–1930. doi: 10.1172/JCI107376. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Naor D., Mishell R. I., Wofsy L. Specific inhibition of an anti-hapten immune response by chemical modifications of cells. J Immunol. 1970 Dec;105(6):1322–1326. [PubMed] [Google Scholar]
  8. Phanuphak P., Moorhead J. W., Claman H. N. Tolerance and contact sensitivity to DNFB in mice. II. Specific in vitro stimulation with a hapten, 2,4-dinitrobenzene sulfonic acid (DNB-SO3Na). J Immunol. 1974 Feb;112(2):849–851. [PubMed] [Google Scholar]
  9. Rubin B. The immune response against hapten-autologous protein conjugates in the mouse. I. Specificity of antibodies produced during the primary response against dinitrophenylated mouse serum albumin. Eur J Immunol. 1973 Jan;3(1):26–32. doi: 10.1002/eji.1830030107. [DOI] [PubMed] [Google Scholar]
  10. Shearer G. M. Cell-mediated cytotoxicity to trinitrophenyl-modified syngeneic lymphocytes. Eur J Immunol. 1974 Aug;4(8):527–533. doi: 10.1002/eji.1830040802. [DOI] [PubMed] [Google Scholar]
  11. Von Boehmer H., Shortman K., Adams P. Nature of the stimulating cell in the syngeneic and the allogeneic mixed lymphocyte reaction in mice. J Exp Med. 1972 Dec 1;136(6):1648–1660. doi: 10.1084/jem.136.6.1648. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WEIGLE W. O. THE INDUCTION OF AUTOIMMUNITY IN RABBITS FOLLOWING INJECTION OF HETEROLOGOUS OR ALTERED HOMOLOGOUS THYROGLOBULIN. J Exp Med. 1965 Feb 1;121:289–308. doi: 10.1084/jem.121.2.289. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. WEIGLE W. O. Termination of acquired immunological tolerance to protein antigens following immunization with altered protein antigens. J Exp Med. 1962 Dec 1;116:913–928. doi: 10.1084/jem.116.6.913. [DOI] [PMC free article] [PubMed] [Google Scholar]

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