Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1975 Nov;29(5):933–944.

Secretory component of the guinea-pig.

J P Vaerman, M C Naccache-Corbic, J F Heremans
PMCID: PMC1445990  PMID: 53196

Abstract

Free secretory component (FSC) was purified from guinea-pig milk by gel-filtration and immunoabsorption on anti-SC antibodies. Guinea-pig FSC cross-reacted with antisera to human FSC, and the reverse. Guinea-pig and human FSC resembled each other in molecular size, electrophoretic mobility and heterogeneity, as well as by the existence of antigenic determinants restricted to the unassociated form of the molecule. Guinea-pig FSC associates in vitro with guinea-pig IgM. Distribution of disulphide links is required to set free guinea-pig FSC from secretory IgA.

Full text

PDF
933

Images in this article

936FIG. 2
on p.936
936FIG. 3
on p.936
937FIG. 4
on p.937
938FIG. 5
on p.938
939FIG. 7
on p.939
940FIG. 8
on p.940
940FIG. 9
on p.940
941FIG. 10
on p.941

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bourne F. J. IgA immunoglobulin from porcine serum. Biochem Biophys Res Commun. 1969 Jul 7;36(1):138–145. doi: 10.1016/0006-291x(69)90660-3. [DOI] [PubMed] [Google Scholar]
  2. Brandtzaeg P. Hidden antigenic determinant in secretory immunoglobulin A. Nature. 1968 Oct 19;220(5164):292–293. doi: 10.1038/220292a0. [DOI] [PubMed] [Google Scholar]
  3. Brandtzaeg P. Unfolding of human secretory immunoglobulin A. Immunochemistry. 1970 Jan;7(1):127–130. doi: 10.1016/0019-2791(70)90037-6. [DOI] [PubMed] [Google Scholar]
  4. Butler J. E. Physicochemical and immunochemical studies on bovine IgA and glycoprotein-a. Biochim Biophys Acta. 1971 Dec 28;251(3):435–449. doi: 10.1016/0005-2795(71)90133-4. [DOI] [PubMed] [Google Scholar]
  5. Cambiaso C. L., Goffinet A., Vaerman J. P., Heremans J. F. Glutaraldehyde-activated aminohexyl- derivative of Sepharose 4B as a new verstile immunoabsorbent. Immunochemistry. 1975 Apr;12(4):273–278. doi: 10.1016/0019-2791(75)90175-5. [DOI] [PubMed] [Google Scholar]
  6. Eskeland T., Brandtzaeg P. Does J chain mediate the combination of 19S IgM and dimeric IgA with the secretory component rather than being necessary for their polymerization? Immunochemistry. 1974 Mar;11(3):161–163. doi: 10.1016/0019-2791(74)90214-6. [DOI] [PubMed] [Google Scholar]
  7. Halpern M. S., Koshland M. E. Noval subunit in secretory IgA. Nature. 1970 Dec 26;228(5278):1276–1278. doi: 10.1038/2281276a0. [DOI] [PubMed] [Google Scholar]
  8. Kobayashi K. Studies on human secretory IgA comparative studies of the IgA-bound secretory piece and the free secretory piece protein. Immunochemistry. 1971 Sep;8(9):785–800. doi: 10.1016/0019-2791(71)90446-0. [DOI] [PubMed] [Google Scholar]
  9. Kobayashi K., Vaerman J. P., Bazin H., LeBacq-Verheyden A. M., Heremans J. F. Identification of J-chain in polymeric immunoglobulins from a variety of species by cross-reaction with rabbit antisera to human J-chain. J Immunol. 1973 Nov;111(5):1590–1594. [PubMed] [Google Scholar]
  10. Kobayashi K., Vaerman J. P., Heremans J. F. Studies on human secretory IgA. II. Comparative studies on a fragment of secretory component derived from secretory IgA and fragments obtained by enzymatic digestion of free secretory component. Immunochemistry. 1973 Feb;10(2):73–80. doi: 10.1016/0019-2791(73)90233-4. [DOI] [PubMed] [Google Scholar]
  11. Lamm M. E., Greenberg J. Human secretory component. Comparison of the form occurring in exocrine immunoglobulin A to the free form. Biochemistry. 1972 Jul 18;11(15):2744–2750. doi: 10.1021/bi00765a002. [DOI] [PubMed] [Google Scholar]
  12. Mach J. P. In vitro combination of human and bovine free secretory component with IgA of various species. Nature. 1970 Dec 26;228(5278):1278–1282. doi: 10.1038/2281278a0. [DOI] [PubMed] [Google Scholar]
  13. Mancini G., Carbonara A. O., Heremans J. F. Immunochemical quantitation of antigens by single radial immunodiffusion. Immunochemistry. 1965 Sep;2(3):235–254. doi: 10.1016/0019-2791(65)90004-2. [DOI] [PubMed] [Google Scholar]
  14. Mestecky J., Kulhavy R., Kraus F. W. Studies on human secretory immunoglobulin A. II. Subunit structure. J Immunol. 1972 Mar;108(3):738–747. [PubMed] [Google Scholar]
  15. O'Daly J. A., Cebra J. J. Rabbit secretory IgA. I. Isolation of secretory component after selective dissociation of the immunoglobulin. J Immunol. 1971 Aug;107(2):436–448. [PubMed] [Google Scholar]
  16. O'Daly J. A., Cebra J. J. Rabbit secretory IgA. II. Free secretory component from colostrum and its specific association with IgA. J Immunol. 1971 Aug;107(2):449–455. [PubMed] [Google Scholar]
  17. Pahud J. J., Mach J. P. Equine secretory IgA and secretory component. Int Arch Allergy Appl Immunol. 1972;42(2):175–186. doi: 10.1159/000230604. [DOI] [PubMed] [Google Scholar]
  18. Pahud J. J., Mach J. P. Identification of secretory IgA, free secretory piece and serum IgA in the ovine and caprine species. Immunochemistry. 1970 Aug;7(8):679–686. doi: 10.1016/0019-2791(70)90174-6. [DOI] [PubMed] [Google Scholar]
  19. Poger M. E., Lamm M. E. Localization of free and bound secretory component in human intestinal epithelial cells. A model for the assembly of secretory IgA. J Exp Med. 1974 Mar 1;139(3):629–642. doi: 10.1084/jem.139.3.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Porter P., Allen W. D. Intestinal IgA in the pig. Experientia. 1970 Jan 15;26(1):90–92. doi: 10.1007/BF01900412. [DOI] [PubMed] [Google Scholar]
  21. Reynolds H. Y., Johnson J. S. Structural units of canine serum and secretory immunoglobulin A. Biochemistry. 1971 Jul 20;10(15):2821–2827. doi: 10.1021/bi00791a003. [DOI] [PubMed] [Google Scholar]
  22. Ricks J., Roberts M., Patterson R. Canine secretory immunoglobulins: identification of secretory component. J Immunol. 1970 Dec;105(6):1327–1333. [PubMed] [Google Scholar]
  23. Tomasi T. B., Jr, Bienenstock J. Secretory immunoglobulins. Adv Immunol. 1968;9:1–96. doi: 10.1016/s0065-2776(08)60441-1. [DOI] [PubMed] [Google Scholar]
  24. Underdown B. J. Studies on the subunit structure of rabbit secretory IgA. J Immunol. 1972 Aug;109(2):227–237. [PubMed] [Google Scholar]
  25. Vaerman J. P., Heremans J. F., Bazin H., Beckers A. Identification and some properties of rat secretory component. J Immunol. 1975 Jan;114(1 Pt 2):265–269. [PubMed] [Google Scholar]
  26. Waldman R. H., Mach J. P., Stella M. M., Rowe D. S. Secretory IgA in human serum. J Immunol. 1970 Jul;105(1):43–47. [PubMed] [Google Scholar]
  27. van Munster P. J., Stoelinga G. B., Clamp J. R., Gerding J. J., Reijnen J. C., Voss M. The molecular weight and the amino acid and carbohydrate compositions of free secretory component (SC). Immunology. 1972 Aug;23(2):249–256. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES