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. 1975 Nov;29(5):885–891.

Induction of PHA response in mouse bone marrow cells by thymic extracts as studied by changes in the structuredness of cytoplasmic matrix.

L Cercek, P Milenkovic, B Cercek, L G Lajtha
PMCID: PMC1445993  PMID: 1081487

Abstract

The specificity and cross-species activity of thymic extracts and some non-specific agents on some alleged properties of T cells and their precursors in the bone marrow cells of normal, nude and thymectomized mice was studied in vitro. The phenomenon of changes in the structuredness of cytoplasmic matrix (SCM) was used to assess the maturation and responses of cells treated with the extracts to mitogens, i.e. phytohaemagglutinin and concanavalin A. We found that thymic extracts of calf and mouse origin induce functional 'maturation' which can be abrogated by cycloheximide, an inhibitor of protein synthesis. The nonspecific agents used (spleen extracts, PPD, dBcAMP and endotoxin) had no effect on bone marrow cells, as measured by changes in the SCM. The method of measuring SCM using the technique of fluorescence polarization is described.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Cercek L., Cercek B. Effect of centrifugal forces on the structuredness of cytoplasm in growing yeast cells. Biophysik. 1973;9(2):105–108. doi: 10.1007/BF01215848. [DOI] [PubMed] [Google Scholar]
  2. Cercek L., Cercek B., Franklin C. I. Biophysical differentiation between lymphocytes from healthy donors, patients with malignant diseases and other disorders. Br J Cancer. 1974 May;29(5):345–352. doi: 10.1038/bjc.1974.81. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Cercek L., Cercek B. Involvement of cyclic-AMP in changes of the structuredness of cytoplasmic matrix (SCM). Radiat Environ Biophys. 1974;11(3):209–212. doi: 10.1007/BF01323189. [DOI] [PubMed] [Google Scholar]
  4. Cercek L., Cercek B., Ockey C. H. Structuredness of the cytoplasmic matrix and Michaelis-Menten constants for the hydrolysis of FDA during the cell cycle in Chinese hamster ovary cells. Biophysik. 1973;10(3):187–194. doi: 10.1007/BF01190577. [DOI] [PubMed] [Google Scholar]
  5. Cercek L., Cercek B. Relationship between changes in the structuredness of cytoplasm and rate constants for the hydrolysis of FDA in Saccharomyces cerevisiae. Biophysik. 1973;9(2):109–112. doi: 10.1007/BF01215849. [DOI] [PubMed] [Google Scholar]
  6. Cercek L., Cercek B. Studies on the structuredness of cytoplasm and rates of enzymatic hydrolysis in growing yeast cells. I. Changes induced by ionizing radiation. Int J Radiat Biol Relat Stud Phys Chem Med. 1972 May;21(5):445–453. doi: 10.1080/09553007214550521. [DOI] [PubMed] [Google Scholar]
  7. Cercek L., Cercek B. Studies on the structuredness of cytoplasm and rates of enzymatic hydrolysis in growing yeast cells. II. Changes induced by ultra-violet light. Int J Radiat Biol Relat Stud Phys Chem Med. 1972 Dec;22(6):539–544. doi: 10.1080/09553007214551441. [DOI] [PubMed] [Google Scholar]
  8. Goldstein A. L., Guha A., Zatz M. M., Hardy M. A., White A. Purification and biological activity of thymosin, a hormone of the thymus gland. Proc Natl Acad Sci U S A. 1972 Jul;69(7):1800–1803. doi: 10.1073/pnas.69.7.1800. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Harris R., Ukaejiofo E. O. Rapid preparation of lymphocytes for tissue-typing. Lancet. 1969 Aug 9;2(7615):327–327. doi: 10.1016/s0140-6736(69)90096-8. [DOI] [PubMed] [Google Scholar]
  10. KAPLAN H. S. Influence of thymectomy, splenectomy, and gonadectomy on incidence of radiation-induced lymphoid tumors in strain C57 black mice. J Natl Cancer Inst. 1950 Aug;11(1):83–90. [PubMed] [Google Scholar]
  11. KUPER S. W., BIGNALL J. R., LUCKCOCK E. D. A quantitative method for studying tumour cells in blood. Lancet. 1961 Apr 22;1(7182):852–853. doi: 10.1016/s0140-6736(61)90177-5. [DOI] [PubMed] [Google Scholar]
  12. Pritchard H., Micklem H. S. Haemopoietic stem cells and progenitors of functional T-lymphocytes in the bone marrow of 'nude' mice. Clin Exp Immunol. 1973 Aug;14(4):597–607. [PMC free article] [PubMed] [Google Scholar]
  13. Scheid M. P., Hoffmann M. K., Komuro K., Hämmerling U., Abbott J., Boyse E. A., Cohen G. H., Hooper J. A., Schulof R. S., Goldstein A. L. Differentiation of T cells induced by preparations from thymus and by nonthymic agents. J Exp Med. 1973 Oct 1;138(4):1027–1032. doi: 10.1084/jem.138.4.1027. [DOI] [PMC free article] [PubMed] [Google Scholar]

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