Figure 3.—

Establishment of repression at telomeres does not occur in cells blocked in G₂/M. (A) G₂/M experimental design. A culture of YSH505 was grown to early log phase in raffinose media. This culture was divided, and half was blocked in G₂/M phase using nocodazole. Following G₂/M arrest the nocodazole-blocked culture was further divided into induced (+ galactose) and uninduced (− galactose) cultures. Galactose was also added at this time to the unblocked cycling cells. (B) Repression does not occur during a G₂/M block. An experiment was performed as described in A. Large-budded cells composed 92% of the population at the time of galactose addition and 96% following 8 hr of galactose induction. Legends indicate whether specific samples were blocked with nocodazole and whether they were induced with galactose. “Hours” indicates the time following the addition of galactose to induced cultures.