Figure 7.—

Lrg1p stimulation of Rho1p GTPase Activity. The assay used purified Rho-GTPase proteins Rho1p, Rho2p, Rho3p, Rho4p, Rho5p, or Cdc42p in the presence or absence of purified Lrg1p GAP protein. The reactions were allowed to proceed for 0 sec, 2 min, 4 min, 8 min, and 16 min. The data plotted for each Rho-GTPase are a reflection of the fold stimulation in GTPase activity in the presence (+) of purified Lrg1 divided by the activity in the absence (−) of Lrg1 (purified vector extract added).