Figure 2.—

Effects of rnlA on dmd mutant-specific cleavages. MH1 and TY0324 cells were grown to 4 × 10⁸ cells/ml and infected with T4 wild-type (+) or dmd mutant (−) phage. Total RNAs were extracted at 21 min after infection and used as templates for primer extension as described in materials and methods. Band F corresponds to full-length soc RNA. Band T corresponds to an RNase E-cleaved product (Otsuka et al. 2003). Arrowheads marked with the position of nucleotide 3′ of the cleavage site indicate bands corresponding to the dmd mutant-specific cleavages (see detail in text).