Figure 4.—

Identification of novel tr-Ac elements in DNA blot analysis. DNA blot analysis was performed with genomic DNA from pooled seedlings derived from two independent ears that each carry the same tr-Ac (lanes 1–18; e.g., lanes 1 and 2 are expected to carry the same tr-Ac, lanes 3 and 4 are expected to carry another tr-Ac), P1-vv (lane 19), and DIG-labeled DNA VII ladder (lane 20; Roche). DNA was digested with EcoRI and hybridized to the internal 700-bp EcoRI/HindIII fragment of Ac. The active, germinally inherited tr-Acs are detected as novel bands present only in the two lines harboring the Ac insertion.