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. 2005 May;170(1):161–171. doi: 10.1534/genetics.104.036343

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Copyright © 2005, Genetics Society of America

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Figure 1.— — Schematics of the Rbf protein and Rbf rescue construct. (A) Diagram of the wild-type Rbf and Rbf^SLS-15 mutant proteins. The mutation analysis of the Rbf^SLS-15 transcripts revealed an 11-bp deletion resulting in a frameshift at amino acid residue 519, followed by the addition of 14 novel residues and truncation of the Rbf protein at residue 533. The truncated protein lacks Pocket B, a highly conserved RBF domain that is required for interactions with partner proteins and the execution of RBF function. (B) Diagram of the Rbf rescue construct and Rbf⁻ clone generation. The Rbf^SLS-15 mutation combined with a Rbf rescue construct allows for the generation of Rbf⁻ clones specifically in the eye, due to eye-specific FLP expression followed by recombination between the FRT sites and subsequent loss of the Rbf⁺ and w⁺ genes. All other tissues, which do not express FLP, remain Rbf⁺, resulting in a rescue of the organismal lethality normally associated with Rbf-deficient flies.

Figure 1.— — Schematics of the Rbf protein and Rbf rescue construct. (A) Diagram of the wild-type Rbf and Rbf^SLS-15 mutant proteins. The mutation analysis of the Rbf^SLS-15 transcripts revealed an 11-bp deletion resulting in a frameshift at amino acid residue 519, followed by the addition of 14 novel residues and truncation of the Rbf protein at residue 533. The truncated protein lacks Pocket B, a highly conserved RBF domain that is required for interactions with partner proteins and the execution of RBF function. (B) Diagram of the Rbf rescue construct and Rbf⁻ clone generation. The Rbf^SLS-15 mutation combined with a Rbf rescue construct allows for the generation of Rbf⁻ clones specifically in the eye, due to eye-specific FLP expression followed by recombination between the FRT sites and subsequent loss of the Rbf⁺ and w⁺ genes. All other tissues, which do not express FLP, remain Rbf⁺, resulting in a rescue of the organismal lethality normally associated with Rbf-deficient flies.