TABLE 4.
Tests functional constraint and adaptation inAdh-Twain
| Fixed nonsynonymous |
Nonsynonymous polymorphism |
Fixed synonymous |
Synonymous polymorphism |
Fisher exact P-value |
G-test | |
|---|---|---|---|---|---|---|
| Unpolarized MK tests | ||||||
| Adh region of Adh-Twain | ||||||
| D. subobscura vs. D. guanche | 22 | 6 | 13 | 15 | 0.0159 | 0.0130 |
| D. subobscura vs. D. madeirensis | 4 | 6 | 6 | 15 | 0.6854 | 0.5248 |
| CG9010 region | ||||||
| D. subobscura vs. D. guanche | 17 | 20 | 5 | 8 | 0.7510 | 0.6400 |
| D. subobscura vs. D. madeirensis | 7 | 20 | 2 | 8 | 0.9999 | 0.7866 |
| Whole gene | ||||||
| D. subobscura vs. D. guanche | 39 | 26 | 18 | 23 | 0.1149 | 0.1055 |
| D. subobscura vs. D. madeirensis | 11 | 26 | 8 | 23 | 0.7905 | 0.7195 |
| Polarized MK tests | ||||||
| D. subobscura Adh region vs. D. guanche Adh | 7 | 6 | 7 | 15 | 0.2882 | 0.1987 |
| D. subobscura CG9010 vs. D. guanche CG9010 | 12 | 20 | 1 | 8 | 0.2283 | 0.1328 |
| D. subobscura Adh-Twain vs. D. guanche Adh-Twain | 19 | 26 | 8 | 23 | 0.1544 | 0.1417 |
| Tests of Adh-Twain vs. ancestors | ||||||
| Adh region vs. Adh | 26 | 6 | 18 | 15 | 0.0332 | 0.0214 |
| CG9010 region vs. CG9010 | 29 | 20 | 8 | 8 | 0.5702 | 0.5195 |
| Polarized | ||||||
| Adh region vs. Adh | 21 | 6 | 7 | 15 | 0.0017 | 0.0012 |
| CG9010 region vs. CG9010 | 25 | 20 | 4 | 8 | 0.2070 | 0.1713 |