Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 May;58(1):157–160.

Production of human monoclonal IgG and IgM antibodies with anti-D (rhesus) specificity using heterohybridomas.

K M Thompson, M D Melamed, K Eagle, B D Gorick, T Gibson, A M Holburn, N C Hughes-Jones
PMCID: PMC1452628  PMID: 3011653

Abstract

Heterohybridomas secreting human IgM and IgG anti-D antibodies of the rhesus blood group system have been established by fusion of EBV-transformed anti-D secreting cells with the mouse myeloma cells X63-Ag8.653. Both classes of antibody reacted with all Rh-positive cells, some Du cells but not with Rh-negative or DB cells. Concentrations of both antibodies reached between 25 micrograms/ml and 50 micrograms/ml in the culture supernatants. The cell lines have been maintained in culture for 14 months and have been shown to be suitable for large-scale production of antibody.

Full text

PDF
157

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Boylston A. W., Gardner B., Anderson R. L., Hughes-Jones N. C. Production of human IgM anti-D in tissue culture by EB-virus-transformed lymphocytes. Scand J Immunol. 1980;12(4):355–358. doi: 10.1111/j.1365-3083.1980.tb00077.x. [DOI] [PubMed] [Google Scholar]
  2. Bron D., Feinberg M. B., Teng N. N., Kaplan H. S. Production of human monoclonal IgG antibodies against Rhesus (D) antigen. Proc Natl Acad Sci U S A. 1984 May;81(10):3214–3217. doi: 10.1073/pnas.81.10.3214. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Croce C. M., Shander M., Martinis J., Cicurel L., D'Ancona G. G., Dolby T. W., Koprowski H. Chromosomal location of the genes for human immunoglobulin heavy chains. Proc Natl Acad Sci U S A. 1979 Jul;76(7):3416–3419. doi: 10.1073/pnas.76.7.3416. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Elson C. J., Bradley J. Human peripheral blood leucocytes forming rosettes with Rhesus (D) isoantigen. Lancet. 1970 Apr 18;1(7651):798–800. doi: 10.1016/s0140-6736(70)92407-4. [DOI] [PubMed] [Google Scholar]
  5. Foung S. K., Perkins S., Raubitschek A., Larrick J., Lizak G., Fishwild D., Engleman E. G., Grumet F. C. Rescue of human monoclonal antibody production from an EBV-transformed B cell line by fusion to a human-mouse hybridoma. J Immunol Methods. 1984 May 11;70(1):83–90. doi: 10.1016/0022-1759(84)90392-2. [DOI] [PubMed] [Google Scholar]
  6. Kozbor D., Roder J. C., Chang T. H., Steplewski Z., Koprowski H. Human anti-tetanus toxoid monoclonal antibody secreted by EBV-transformed human B cells fused with murine myeloma. Hybridoma. 1982;1(3):323–328. doi: 10.1089/hyb.1.1982.1.323. [DOI] [PubMed] [Google Scholar]
  7. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  8. Lane H. C., Shelhamer J. H., Mostowski H. S., Fauci A. S. Human monoclonal anti-keyhole limpet hemocyanin antibody-secreting hybridoma produced from peripheral blood B lymphocytes of a keyhole limpet hemocyanin-immune individual. J Exp Med. 1982 Jan 1;155(1):333–338. doi: 10.1084/jem.155.1.333. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Melamed M. D., Gordon J., Ley S. J., Edgar D., Hughes-Jones N. C. Senescence of a human lymphoblastoid clone producing anti-Rhesus(D). Eur J Immunol. 1985 Jul;15(7):742–746. doi: 10.1002/eji.1830150720. [DOI] [PubMed] [Google Scholar]
  10. Nowinski R., Berglund C., Lane J., Lostrom M., Bernstein I., Young W., Hakomori S. I., Hill L., Cooney M. Human monoclonal antibody against Forssman antigen. Science. 1980 Oct 31;210(4469):537–539. doi: 10.1126/science.7423202. [DOI] [PubMed] [Google Scholar]
  11. Ruddle F. H. Linkage analysis in man by somatic cell genetics. Nature. 1973 Mar 16;242(5394):165–169. doi: 10.1038/242165a0. [DOI] [PubMed] [Google Scholar]
  12. Schlom J., Wunderlich D., Teramoto Y. A. Generation of human monoclonal antibodies reactive with human mammary carcinoma cells. Proc Natl Acad Sci U S A. 1980 Nov;77(11):6841–6845. doi: 10.1073/pnas.77.11.6841. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schwaber J., Cohen E. P. Human x mouse somatic cell hybrid clone secreting immunoglobulins of both parental types. Nature. 1973 Aug 17;244(5416):444–447. doi: 10.1038/244444a0. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES