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. 1986 May;58(1):105–110.

Comparative evaluation of the effect of pharmacological agents on endocytosis and coendocytosis of IgE by rat basophilic leukaemia cells.

K Furuichi, C Ra, C Isersky, J Rivera
PMCID: PMC1452636  PMID: 3011652

Abstract

The aggregation of IgE bound to rat basophilic leukaemia (RBL) cells leads to the exocytosis of mediators, the endocytosis of the antigen-aggregated mouse IgE anti-DNP, as well as the coendocytosis of some unaggregated monomeric rat IgE (IR162) and/or unbound receptors. We describe here the relative effect on endocytosis and coendocytosis of various pharmacological agents that block or enhance exocytosis. We have previously shown that, unlike exocytosis, endocytosis by RBL and normal rat mast cells was independent of extracellular calcium. We show here that the presence of calcium chelators or antagonists also had no effect on endocytosis of cross-linked IgE. However, coendocytosis of non-cross-linked IgE was partially inhibited by the elimination of extracellular calcium and the addition of calcium chelators such as EDTA or EGTA-Mg2+. Moreover, the addition of calcium antagonists such as Ni2+ and Co2+ (5 mM) to an incubation mixture containing Ca2+ (1 mM) resulted in the complete inhibition of coendocytosis without affecting endocytosis. Other inhibitors of exocytosis such as sodium azide (10-2M), quercetin (10-4M) and dibutyryl cyclic AMP (10-2 M) blocked coendocytosis completely but had no effect on endocytosis. Sodium azide (10 mM) in combination with 2-deoxyglucose (10 mM) effectively inhibited (90%) endocytosis. Cytochalasin B (10-4 M), which was shown to enhance serotonin release, had no effect on the extent of endocytosis or coendocytosis observed 20 min after the initiation of aggregation. Thus, in RBL cells, endocytosis, coendocytosis and exocytosis exhibit distinguishable sensitivities to some pharmacological drugs.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barsumian E. L., Isersky C., Petrino M. G., Siraganian R. P. IgE-induced histamine release from rat basophilic leukemia cell lines: isolation of releasing and nonreleasing clones. Eur J Immunol. 1981 Apr;11(4):317–323. doi: 10.1002/eji.1830110410. [DOI] [PubMed] [Google Scholar]
  2. Bazin H., Querinjean P., Beckers A., Heremans J. F., Dessy F. Transplantable immunoglobulin-secreting tumours in rats. IV. Sixty-three IgE-secreting immunocytoma tumours. Immunology. 1974 Apr;26(4):713–723. [PMC free article] [PubMed] [Google Scholar]
  3. Beaven M. A., Rogers J., Moore J. P., Hesketh T. R., Smith G. A., Metcalfe J. C. The mechanism of the calcium signal and correlation with histamine release in 2H3 cells. J Biol Chem. 1984 Jun 10;259(11):7129–7136. [PubMed] [Google Scholar]
  4. Furuichi K., Rivera J., Isersky C. The fate of IgE bound to rat basophilic leukemia cells. III. Relationship between antigen-induced endocytosis and serotonin release. J Immunol. 1984 Sep;133(3):1513–1520. [PubMed] [Google Scholar]
  5. Furuichi K., Rivera J., Triche T., Isersky C. The fate of IgE bound to rat basophilic leukemia cells. IV. Functional association between the receptors for IgE. J Immunol. 1985 Mar;134(3):1766–1773. [PubMed] [Google Scholar]
  6. Goldstein J. L., Anderson R. G., Brown M. S. Coated pits, coated vesicles, and receptor-mediated endocytosis. Nature. 1979 Jun 21;279(5715):679–685. doi: 10.1038/279679a0. [DOI] [PubMed] [Google Scholar]
  7. Gomperts B. D., Cockcroft S., Bennett J. P., Fewtrell C. M. Early events in the activation of Ca2+ dependent secretion: studies with rat peritoneal mast cells. J Physiol (Paris) 1980 Sep;76(5):383–393. [PubMed] [Google Scholar]
  8. Holowka D., Metzger H. Further characterization of the beta-component of the receptor for immunoglobulin E. Mol Immunol. 1982 Feb;19(2):219–227. doi: 10.1016/0161-5890(82)90334-0. [DOI] [PubMed] [Google Scholar]
  9. Isersky C., Kulczycki A., Jr, Metzger H. Isolation of IgE from reaginic rat serum. J Immunol. 1974 May;112(5):1909–1919. [PubMed] [Google Scholar]
  10. Isersky C., Rivera J., Mims S., Triche T. J. The fate of IgE bound to rat basophilic leukemia cells. J Immunol. 1979 May;122(5):1926–1936. [PubMed] [Google Scholar]
  11. Isersky C., Rivera J., Segal D. M., Triche T. The fate of IgE bound to rat basophilic leukemia cells. II. Endocytosis of IgE oligomers and effect on receptor turnover. J Immunol. 1983 Jul;131(1):388–396. [PubMed] [Google Scholar]
  12. Isersky C., Taurog J. D., Poy G., Metzger H. Triggering of cultured neoplastic mast cells by antibodies to the receptor for IgE. J Immunol. 1978 Aug;121(2):549–558. [PubMed] [Google Scholar]
  13. Kazimierczak W., Diamant B. Mechanisms of histamine release in anaphylactic and anaphylactoid reactions. Prog Allergy. 1978;24:295–365. [PubMed] [Google Scholar]
  14. Liu F. T., Bohn J. W., Ferry E. L., Yamamoto H., Molinaro C. A., Sherman L. A., Klinman N. R., Katz D. H. Monoclonal dinitrophenyl-specific murine IgE antibody: preparation, isolation, and characterization. J Immunol. 1980 Jun;124(6):2728–2737. [PubMed] [Google Scholar]
  15. Metzger H. The receptor on mast cells and related cells with high affinity for IgE. Contemp Top Mol Immunol. 1983;9:115–145. doi: 10.1007/978-1-4684-4517-6_4. [DOI] [PubMed] [Google Scholar]
  16. Middleton E., Jr, Drzewiecki G., Krishnarao D. Quercetin: an inhibitor of antigen-induced human basophil histamine release. J Immunol. 1981 Aug;127(2):546–550. [PubMed] [Google Scholar]
  17. Pearce F. L. Calcium and histamine secretion from mast cells. Prog Med Chem. 1982;19:59–109. doi: 10.1016/s0079-6468(08)70328-x. [DOI] [PubMed] [Google Scholar]
  18. Peng H. B. Participation of calcium and calmodulin in the formation of acetylcholine receptor clusters. J Cell Biol. 1984 Feb;98(2):550–557. doi: 10.1083/jcb.98.2.550. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Salisbury J. L., Condeelis J. S., Satir P. Role of coated vesicles, microfilaments, and calmodulin in receptor-mediated endocytosis by cultured B lymphoblastoid cells. J Cell Biol. 1980 Oct;87(1):132–141. doi: 10.1083/jcb.87.1.132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Silverstein S. C., Steinman R. M., Cohn Z. A. Endocytosis. Annu Rev Biochem. 1977;46:669–722. doi: 10.1146/annurev.bi.46.070177.003321. [DOI] [PubMed] [Google Scholar]
  21. Sobotka A. K., Dembo M., Goldstein B., Lichtenstein L. M. Antigen-specific desensitization of human basophils. J Immunol. 1979 Feb;122(2):511–517. [PubMed] [Google Scholar]

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