Abstract
C3 fragment opsonization and chemiluminescence were assessed using two yeasts (Saccharomyces cerevisiae and Candida albicans) and two strains of bacteria (Staphylococcus aureus and Escherichia coli) following incubation with sera previously shown to be either efficient or poor in the opsonization of baker's yeast. Sera with poor function consistently fixed lower levels of C3 fragments and generated less chemiluminescence with all four organisms than sera with normal function. The differences were most marked with the two yeasts. We conclude that sera with normal complement function but manifesting a relatively common defect in the opsonization of baker's yeast have a general opsonic defect.
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- Brown E. J., Joiner K. A., Cole R. M., Berger M. Localization of complement component 3 on Streptococcus pneumoniae: anti-capsular antibody causes complement deposition on the pneumococcal capsule. Infect Immun. 1983 Jan;39(1):403–409. doi: 10.1128/iai.39.1.403-409.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Engels W., Endert J., Van Boven C. P. A quantitative method for assessing the third complement factor (C3) attached to the surface of opsonized Pseudomonas aeruginosa: interrelationship between C3 fixation, phagocytosis and complement consumption. J Immunol Methods. 1985 Jul 16;81(1):43–53. doi: 10.1016/0022-1759(85)90120-6. [DOI] [PubMed] [Google Scholar]
- Kerr M. A., Falconer J. S., Bashey A., Beck J. S. The effect of C3 levels on yeast opsonization by normal and pathological sera: identification of a complement independent opsonin. Clin Exp Immunol. 1983 Dec;54(3):793–800. [PMC free article] [PubMed] [Google Scholar]
- Levinsky R. J., Harvey B. A., Paleja S. A rapid objective method for measuring the yeast opsonisation activity of serum. J Immunol Methods. 1978;24(3-4):251–256. doi: 10.1016/0022-1759(78)90129-1. [DOI] [PubMed] [Google Scholar]
- Richardson V. F., Larcher V. F., Price J. F. A common congenital immunodeficiency predisposing to infection and atopy in infancy. Arch Dis Child. 1983 Oct;58(10):799–802. doi: 10.1136/adc.58.10.799. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Roberton D. M., Dhanjal N. K., Levinsky R. J., Mowbray J. F., Turner M. W. Polymorphonuclear neutrophil iodination response as an estimate of defective yeast opsonization. Clin Exp Immunol. 1981 Jan;43(1):208–214. [PMC free article] [PubMed] [Google Scholar]
- Shields J. G., Turner M. W. The importance of antibody quality in sandwich ELISA systems. Evaluation of selected commercial reagents. J Immunol Methods. 1986 Feb 27;87(1):29–33. doi: 10.1016/0022-1759(86)90340-6. [DOI] [PubMed] [Google Scholar]
- Soothill J. F., Harvey B. A. Defective opsonization. A common immunity deficiency. Arch Dis Child. 1976 Feb;51(2):91–99. doi: 10.1136/adc.51.2.91. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Turner M. W., Mowbray J. F., Roberton D. R. A study of C3b deposition on yeast surfaces by sera of known opsonic potential. Clin Exp Immunol. 1981 Nov;46(2):412–419. [PMC free article] [PubMed] [Google Scholar]
- Turner M. W., Seymour N. D., Kazatchkine M. D., Mowbray J. F. Suboptimal C3b/C3bi deposition and defective yeast opsonization. I. Evidence for the absence of essential co-factor activity. Clin Exp Immunol. 1985 Nov;62(2):427–434. [PMC free article] [PubMed] [Google Scholar]
- Turner M. W., Seymour N. D., Kazatchkine M. D., Mowbray J. F. Suboptimal C3b/C3bi deposition and defective yeast opsonization. II. Partial purification and preliminary characterization of an opsonic co-factor able to correct sera with the defect. Clin Exp Immunol. 1985 Nov;62(2):435–441. [PMC free article] [PubMed] [Google Scholar]