Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 Sep;59(1):87–93.

Enhanced granulocyte cytotoxicity by mediators derived from anti-IgE-stimulated human leucocytes.

R Moqbel, A J MacDonald, A B Kay
PMCID: PMC1453124  PMID: 2428736

Abstract

Basophil-containing leucocyte fractions stimulated with an anti-human IgE, F(ab')2, generated histamine and the leukotrienes LTB4 and LTC4 with significant correlations between LTB4 and histamine (P less than 0.01; n = 26) and LTC4 and histamine release (P less than 0.001; n = 29) in the cell-free supernatants (SN). SN from these anti-IgE-treated cells enhanced the cytotoxicity of eosinophils and neutrophils (against complement-coated schistosomula of Schistosoma mansoni) in vitro. When SN were fractionated by reverse phase-high performance liquid chromatography (RP-HPLC), the enhancing activity for neutrophils was almost totally confined to fractions having LTB4 immunoreactivity (co-eluting as a single peak with the synthetic LTB4 marker). In contrast, the LTB4-containing fraction had minimal effects on eosinophil cytotoxicity, whereas synthetic histamine gave comparable enhancement to the unfractionated SN. The generation of LTs (but not histamine), as well as enhanced neutrophil cytotoxicity from basophil-containing leucocytes by anti-IgE treatment, was maximally inhibited by the 5-lipoxygenase inhibitors U-60, 257 and BW755C. Conversely, the cyclooxygenase inhibitor indomethacin did not significantly affect LT release, nor did it affect the subsequent cytotoxicity enhancing activity of SN from such cells. These results indicate that LTB4 and LTC4 are released from basophils, together with histamine, by IgE-dependent mechanisms, LTB4 enhances the cytotoxicity of bystander neutrophils, and histamine (and to a lesser extent, LTB4) augments eosinophil cytotoxicity.

Full text

PDF
87

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anwar A. R., Kay B. Enhancement of human eosinophil complement receptors by pharmacologic mediators. J Immunol. 1978 Oct;121(4):1245–1250. [PubMed] [Google Scholar]
  2. Anwar A. R., McKean J. R., Smithers S. R., Kay A. B. Human eosinophil- and neutrophil-mediated killing of schistosomula of Schistosoma mansoni in vitro. I. Enhancement of complement-dependent damage by mast cell-derived mediators and formyl methionyl peptides. J Immunol. 1980 Mar;124(3):1122–1129. [PubMed] [Google Scholar]
  3. Anwar A. R., Smithers S. R., Kay A. B. Killing of schistosomula of Schistosoma mansoni coated with antibody and/or complement by human leukocytes in vitro: requirement for complement in preferential killing by eosinophils. J Immunol. 1979 Feb;122(2):628–637. [PubMed] [Google Scholar]
  4. David J. R., Vadas M. A., Butterworth A. E., de Brito P. A., Carvalho E. M., David R. A., Bina J. C., Andrade Z. A. Enhanced helminthotoxic capacity of eosinophils from patients with eosinophilia. N Engl J Med. 1980 Nov 13;303(20):1147–1152. doi: 10.1056/NEJM198011133032004. [DOI] [PubMed] [Google Scholar]
  5. Gilbert H. S., Ornstein L. Basophil counting with a new staining method using alcian blue. Blood. 1975 Aug;46(2):279–286. [PubMed] [Google Scholar]
  6. Hayes E. C., Lombardo D. L., Girard Y., Maycock A. L., Rokach J., Rosenthal A. S., Young R. N., Egan R. W., Zweerink H. J. Measuring leukotrienes of slow reacting substance of anaphylaxis: development of a specific radioimmunoassay. J Immunol. 1983 Jul;131(1):429–433. [PubMed] [Google Scholar]
  7. Kay A. B., Glass E. J., Salter D. M. Leucoattractants enhance complement receptors on human phagocytic cells. Clin Exp Immunol. 1979 Nov;38(2):294–299. [PMC free article] [PubMed] [Google Scholar]
  8. Kimura I., Moritani Y., Tanizaki Y. Basophils in bronchial asthma with reference to reagin-type allergy. Clin Allergy. 1973 Jun;3(2):195–202. doi: 10.1111/j.1365-2222.1973.tb01321.x. [DOI] [PubMed] [Google Scholar]
  9. Melewicz F. M., Spiegelberg H. L. Fc receptors for IgE on a subpopulation of human peripheral blood monocytes. J Immunol. 1980 Sep;125(3):1026–1031. [PubMed] [Google Scholar]
  10. Moqbel R., Durham S. R., Shaw R. J., Walsh G. M., MacDonald A. J., Mackay J. A., Carroll M. P., Kay A. B. Enhancement of leukocyte cytotoxicity after exercise-induced asthma. Am Rev Respir Dis. 1986 Apr;133(4):609–613. doi: 10.1164/arrd.1986.133.4.609. [DOI] [PubMed] [Google Scholar]
  11. Moqbel R., MacDonald A. J., Kay A. B. Enhancement of human eosinophil- and neutrophil-mediated killing of schistosomula of Schistosoma mansoni by reversed type (IgE-mediated) anaphylaxis, in vitro. Clin Exp Immunol. 1985 Mar;59(3):577–586. [PMC free article] [PubMed] [Google Scholar]
  12. Moqbel R., Sass-Kuhn S. P., Goetzl E. J., Kay A. B. Enhancement of neutrophil- and eosinophil-mediated complement-dependent killing of schistosomula of Schistosoma mansoni in vitro by leukotriene B4. Clin Exp Immunol. 1983 Jun;52(3):519–527. [PMC free article] [PubMed] [Google Scholar]
  13. Ramalho-Pinto F. J., Gazzinelli G., Howells R. E., Mota-Santos T. A., Figueiredo E. A., Pellegrino J. Schistosoma mansoni: defined system for stepwise transformation of cercaria to schistosomule in vitro. Exp Parasitol. 1974 Dec;36(3):360–372. doi: 10.1016/0014-4894(74)90076-9. [DOI] [PubMed] [Google Scholar]
  14. Siraganian R. P., Brodsky M. J. Automated histamine analysis for in vitro allergy testing. I. A method utilizing allergen-induced histamine release from whole blood. J Allergy Clin Immunol. 1976 Jun;57(6):525–540. doi: 10.1016/0091-6749(76)90003-8. [DOI] [PubMed] [Google Scholar]
  15. Smithers S. R., Terry R. J. The infection of laboratory hosts with cercariae of Schistosoma mansoni and the recovery of the adult worms. Parasitology. 1965 Nov;55(4):695–700. doi: 10.1017/s0031182000086248. [DOI] [PubMed] [Google Scholar]
  16. Sánchez-Crespo M., Alonso F., Egido J. Platelet-activating factor in anaphylaxis and phagocytosis. I. Release from human peripheral polymorphonuclears and monocytes during the stimulation by ionophore A23187 and phagocytosis but not from degranulating basophils. Immunology. 1980 Aug;40(4):645–655. [PMC free article] [PubMed] [Google Scholar]
  17. Vadas M. A., David J. R., Butterworth A., Pisani N. T., Siongok T. A. A new method for the purification of human eosinophils and neutrophils, and a comparison of the ability of these cells to damage schistosomula of Schistosoma mansoni. J Immunol. 1979 Apr;122(4):1228–1236. [PubMed] [Google Scholar]
  18. Williams J. D., Czop J. K., Austen K. F. Release of leukotrienes by human monocytes on stimulation of their phagocytic receptor for particulate activators. J Immunol. 1984 Jun;132(6):3034–3040. [PubMed] [Google Scholar]
  19. Young R. N., Kakushima M., Rokach J. Studies on the preparation of conjugates of leukotriene C4 with proteins for development of an immunoassay for SRS-A (1). Prostaglandins. 1982 Apr;23(4):603–613. doi: 10.1016/0090-6980(82)90120-4. [DOI] [PubMed] [Google Scholar]
  20. Young R. N., Zamboni R., Rokach J. Studies on the conjugation of leukotriene B4 with proteins for development of a radioimmunoassay for leukotriene B4. Prostaglandins. 1983 Oct;26(4):605–613. doi: 10.1016/0090-6980(83)90197-1. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES