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. 1986 Sep;59(1):95–99.

Immunological heterogeneity of human monocyte subsets prepared by counterflow centrifugation elutriation.

A H Esa, S J Noga, A D Donnenberg, A D Hess
PMCID: PMC1453127  PMID: 2944818

Abstract

Human peripheral blood mononuclear cells were separated into three fractions by means of counterflow centrifugation elutriation (CCE). The first fraction, eluted at a flow rate of 24 ml/min, was composed of lymphocytes with less than 2% contaminating esterase-positive cells. The cells in this fraction were incapable of responding to either soluble antigen (tetanus toxoid) or particulate antigen (cytomegalovirus-infected fibroblasts) unless recombined with accessory cells. The second fraction, eluted at a flow rate of 28 ml/min, was composed predominantly (72%) of small Ia, leu M3, and esterase-positive monocytes, which stained weakly with leu 10 antibody. Cells in this fraction efficiently presented soluble and particulate antigens to monocyte-depleted lymphocytes. Of the remaining cells, 87% were large esterase-positive monocytes that labelled strongly with Ia, leu M3, and leu 10. These cells were less efficient in antigen presentation than the small monocytes. However, lymphocytes activated with antigen-pulsed large monocytes exhibited more suppressor cell activity than those activated with antigen-pulsed small monocytes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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