Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 Nov;59(3):467–471.

Immunogenic and antigenic epitopes of immunoglobulins. XVIII. Subpopulations of human lambda chains defined with a panel of monoclonal antibodies.

M R Walker, A Solomon, N R Ling, B Brown, J A Lowe, D Hardie, R Jefferis
PMCID: PMC1453201  PMID: 2432000

Abstract

Following immunization with various lambda Bence-Jones proteins, over 100 hybridomas were established. A majority of the monoclonal antibodies (McAb) produced were demonstrated to recognize epitopes common to all lambda chains or unique to the immunogen. Twenty-nine McAb exhibited profiles of 'restricted' reactivity. Analysis with a panel of serologically defined antigens allowed McAb recognizing the lambda II subgroup to be identified. Other McAb recognized epitopes selectively expressed by lambda II and lambda III subgroup proteins. A further twenty-one McAb exhibited individual specificity profiles, some of which detect minor lambda chain subpopulations. Several of these McAb have potential for analysis of the clonality of specific antibody responses and for detecting monoclonal B-cell proliferations.

Full text

PDF
467

Images in this article

469Figure 1
on p.469

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  2. Karlsson F. A., Björk I., Berggård I. Recovery of the native conformations of the variable and constant halves of an immunoglobulin light chain upon renaturation from the linear random coil state. Immunochemistry. 1972 Nov;9(11):1129–1138. doi: 10.1016/0019-2791(72)90083-3. [DOI] [PubMed] [Google Scholar]
  3. Ling N. R., Bishop S., Jefferis Use of antibody-coated red cells for the sensitive detection of antigen and in rosette tests for cells bearing surface immunoglobulins. J Immunol Methods. 1977;15(3):279–289. doi: 10.1016/0022-1759(77)90065-5. [DOI] [PubMed] [Google Scholar]
  4. Ling N. R., Lowe J., Hardie D., Evans S., Jefferis R. Detection of free kappa chains in human serum and urine using pairs of monoclonal antibodies reacting with C kappa epitopes not available on whole immunoglobulins. Clin Exp Immunol. 1983 Apr;52(1):234–240. [PMC free article] [PubMed] [Google Scholar]
  5. Lowe J., Hardie D., Jefferis R., Ling N. R., Drysdale P., Richardson P., Raykundalia C., Catty D., Appleby P., Drew R. Properties of monoclonal antibodies to human immunoglobulin kappa and lambda chains. Immunology. 1981 Apr;42(4):649–659. [PMC free article] [PubMed] [Google Scholar]
  6. Moynihan J. A., Looney R. J., Abraham G. N. The VKIIIb light chain sub-subgroup: restricted association with mu heavy chain in normal human serum. Immunology. 1985 Feb;54(2):207–213. [PMC free article] [PubMed] [Google Scholar]
  7. Reimer C. B., Phillips D. J., Aloisio C. H., Moore D. D., Galland G. G., Wells T. W., Black C. M., McDougal J. S. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma. 1984 Fall;3(3):263–275. doi: 10.1089/hyb.1984.3.263. [DOI] [PubMed] [Google Scholar]
  8. Seon B. K., Roholt O. A., Pressman D. Differences in the enzymatic digestibility of the variable and constant halves of Bence-Jones protein with temperature. J Biol Chem. 1972 Apr 10;247(7):2151–2155. [PubMed] [Google Scholar]
  9. Solomon A. Light chains of human immunoglobulins. Methods Enzymol. 1985;116:101–121. doi: 10.1016/s0076-6879(85)16008-8. [DOI] [PubMed] [Google Scholar]
  10. Steensgaard J., Jacobsen C., Lowe J., Hardie D., Ling N. R., Jefferis R. The development of difference turbidimetric analysis for monoclonal antibodies to human IgG. Mol Immunol. 1980 Oct;17(10):1315–1318. doi: 10.1016/0161-5890(80)90027-9. [DOI] [PubMed] [Google Scholar]
  11. Walker M., Hardie D., Lowe J., Ling N. R., De Lange G., Jefferis R. Immunogenicity and antigenicity of immunoglobulins. XII. Intact light chain and heavy chain isotype-restricted Vk-associated epitopes. Immunology. 1985 Jun;55(2):205–211. [PMC free article] [PubMed] [Google Scholar]
  12. Zhu D., Kim H. S., Deutsch H. F. Variable-region sequences of five human lambda-chain proteins reacting with an idiotypic antibody to the MCG Bence-Jones protein. Mol Immunol. 1983 Oct;20(10):1107–1116. doi: 10.1016/0161-5890(83)90120-7. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES