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. 1986 Nov;59(3):379–382.

Effects on murine T helper cell proliferation by DTH activated syngeneic epidermal cells.

A Scheynius, R Holmdahl, L Klareskog
PMCID: PMC1453203  PMID: 2431997

Abstract

Epidermal cells, obtained either from the site of a delayed type of hypersensitivity (DTH) reaction to 2,4-dinitro-1-fluorobenzene (DNFB) or from skin subjected only to painting with acetone: olive oil have been studied for their capacity to stimulate antigen specific T-lymphocyte proliferation. Small numbers of cells from the control, as well as from the DTH activated epidermis, presented collagen type II to a line of and a clone of collagen II specific T helper cells. Larger numbers of epidermal cells from DTH activated epidermis suppressed this T-cell proliferation compared to control epidermal cells. Phenotypic analysis showed an increased expression of Thy-1 antigen on epidermal cells from DTH lesions 3 days after provocation. It is suggested that epidermal cells may be involved in negative as well as positive feedback loops in conjunction with T-cell immunity. It would thus be of interest to study further whether the induced Thy-1 antigen expression on epidermal cells is of importance in a negative feedback system.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiba S., Aizawa H., Obata M., Tagami H. Dynamic changes in epidermal Ia-positive cells in allergic contact sensitivity reactions in mice. Br J Dermatol. 1984 Nov;111(5):507–516. doi: 10.1111/j.1365-2133.1984.tb06619.x. [DOI] [PubMed] [Google Scholar]
  2. Barclay A. N., Mason D. W. Induction of Ia antigen in rat epidermal cells and gut epithelium by immunological stimuli. J Exp Med. 1982 Dec 1;156(6):1665–1676. doi: 10.1084/jem.156.6.1665. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bergstresser P. R., Tigelaar R. E., Dees J. H., Streilein J. W. Thy-1 antigen-bearing dendritic cells populate murine epidermis. J Invest Dermatol. 1983 Sep;81(3):286–288. doi: 10.1111/1523-1747.ep12518332. [DOI] [PubMed] [Google Scholar]
  4. Courtenay J. S., Dallman M. J., Dayan A. D., Martin A., Mosedale B. Immunisation against heterologous type II collagen induces arthritis in mice. Nature. 1980 Feb 14;283(5748):666–668. doi: 10.1038/283666a0. [DOI] [PubMed] [Google Scholar]
  5. Holmdahl R., Klareskog L., Rubin K., Larsson E., Wigzell H. T lymphocytes in collagen II-induced arthritis in mice. Characterization of arthritogenic collagen II-specific T-cell lines and clones. Scand J Immunol. 1985 Sep;22(3):295–306. doi: 10.1111/j.1365-3083.1985.tb01884.x. [DOI] [PubMed] [Google Scholar]
  6. Holmdahl R., Rubin K., Klareskog L., Larsson E., Wigzell H. Characterization of the antibody response in mice with type II collagen-induced arthritis, using monoclonal anti-type II collagen antibodies. Arthritis Rheum. 1986 Mar;29(3):400–410. doi: 10.1002/art.1780290314. [DOI] [PubMed] [Google Scholar]
  7. Hsu S. M., Raine L., Fanger H. Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem. 1981 Apr;29(4):577–580. doi: 10.1177/29.4.6166661. [DOI] [PubMed] [Google Scholar]
  8. Kaplow I. S. Letter: Substitute for benzidine in myeloperoxidase stains. Am J Clin Pathol. 1975 Mar;63(3):451–451. doi: 10.1093/ajcp/63.3.451. [DOI] [PubMed] [Google Scholar]
  9. Klareskog L., Holmdahl R., Rubin K., Victorin A., Lindgren J. A. Different populations of rheumatoid adherent cells mediate activation versus suppression of T lymphocyte proliferation. Arthritis Rheum. 1985 Aug;28(8):863–872. doi: 10.1002/art.1780280805. [DOI] [PubMed] [Google Scholar]
  10. Klareskog L., Tjernlund U., Forsum U., Peterson P. A. Epidermal Langerhans cells express Ia antigens. Nature. 1977 Jul 21;268(5617):248–250. doi: 10.1038/268248a0. [DOI] [PubMed] [Google Scholar]
  11. Metzger Z., Hoffeld J. T., Oppenheim J. J. Macrophage-mediated suppression. I. Evidence for participation of both hdyrogen peroxide and prostaglandins in suppression of murine lymphocyte proliferation. J Immunol. 1980 Feb;124(2):983–988. [PubMed] [Google Scholar]
  12. Phanuphak P., Moorhead J. W., Claman H. N. Tolerance and contact sensitivity to DNFB in mice. I. In vivo detection by ear swelling and correlation with in vitro cell stimulation. J Immunol. 1974 Jan;112(1):115–123. [PubMed] [Google Scholar]
  13. Rowden G., Lewis M. G., Sullivan A. K. Ia antigen expression on human epidermal Langerhans cells. Nature. 1977 Jul 21;268(5617):247–248. doi: 10.1038/268247a0. [DOI] [PubMed] [Google Scholar]
  14. Scheynius A., Klareskog L., Forsum U. In situ identification of T lymphocyte subsets and HLA-DR expressing cells in the human skin tuberculin reaction. Clin Exp Immunol. 1982 Aug;49(2):325–330. [PMC free article] [PubMed] [Google Scholar]
  15. Scheynius A., Klareskog L., Forsum U., Matsson P., Karlsson L., Peterson P. A., Sundström C. Enrichment of epidermal Langerhans cells: studies with a monolayer technique and flow cytometry sorting. J Invest Dermatol. 1982 Jun;78(6):452–455. doi: 10.1111/1523-1747.ep12510125. [DOI] [PubMed] [Google Scholar]
  16. Scheynius A., Klareskog L., Holmdahl R., Larsson P. Induction of Thy-1 antigen on murine keratinocytes. Immunology. 1986 Mar;57(3):451–454. [PMC free article] [PubMed] [Google Scholar]
  17. Silberberg-Sinakin I., Gigli I., Baer R. L., Thorbecke G. J. Langerhans cells: role in contact hypersensitivity and relationship to lymphoid dendritic cells and to macrophages. Immunol Rev. 1980;53:203–232. doi: 10.1111/j.1600-065x.1980.tb01045.x. [DOI] [PubMed] [Google Scholar]
  18. Suitters A. J., Lampert I. A. Expression of Ia antigen on epidermal keratinocytes is a consequence of cellular immunity. Br J Exp Pathol. 1982 Apr;63(2):207–213. [PMC free article] [PubMed] [Google Scholar]
  19. Tjernlund U. Beta-2-microglobulin and HLA-DR(Ia)-antigens in normal and pathological skin. Acta Derm Venereol Suppl (Stockh) 1981;94:1–40. [PubMed] [Google Scholar]
  20. Tschachler E., Schuler G., Hutterer J., Leibl H., Wolff K., Stingl G. Expression of Thy-1 antigen by murine epidermal cells. J Invest Dermatol. 1983 Sep;81(3):282–285. doi: 10.1111/1523-1747.ep12518326. [DOI] [PubMed] [Google Scholar]
  21. Unanue E. R. Antigen-presenting function of the macrophage. Annu Rev Immunol. 1984;2:395–428. doi: 10.1146/annurev.iy.02.040184.002143. [DOI] [PubMed] [Google Scholar]
  22. Warnke R., Levy R. Detection of T and B cell antigens hybridoma monoclonal antibodies: a biotin-avidin-horseradish peroxidase method. J Histochem Cytochem. 1980 Aug;28(8):771–776. doi: 10.1177/28.8.7003003. [DOI] [PubMed] [Google Scholar]

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