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. 1987 May;61(1):11–14.

Biosynthesis and secretion of the third component of complement by human endothelial cells in vitro.

A Ueki, T Sai, H Oka, M Tabata, K Hosokawa, Y Mochizuki
PMCID: PMC1453310  PMID: 3108140

Abstract

The third component of complement (C3) is synthesized and released by cultured human capillary endothelial cells. After the incubation of cells in a methionine-free medium containing 35S-methionine for 48 hr, culture supernatants were immunoprecipitated with anti-human C3 serum. SDS solubilization and 2-ME reduction of the immunoprecipitates, followed by separation with SDS-polyacrylamide gel electrophoresis and autoradiography, revealed two major bands comparable to those of the alpha and beta chains of human C3. The content of C3 in the culture medium harvested at different time-intervals was determined by ELISA. The C3 secretion rate was about 250 ng/10(6) cells/5 days in the primary culture medium and 75 ng/10(6) cells/5 days after seven passages. The capillary endothelial cells described here have factor VIIIRAg specific for endothelial cells, and exhibit ring formation resembling capillary lumina, but they lack Weibel-Palade bodies.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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