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. 1987 Jan;60(1):19–28.

Immunocompetence of normal human appendiceal lymphoid cells: in vitro studies.

H Kawanishi
PMCID: PMC1453345  PMID: 3493205

Abstract

In order to study the activity of immunocompetent lymphocytes in normal human adult mucosal lymphoid tissues in the gut, viable mononuclear cells (MNC) from surgically removed appendices were isolated with a rapid and improved technique, and their immune reactivity determined in vitro. Nearly two-thirds of the isolated cells were B cells, composed of sIgM (30%), sIgA (19%) and sIgG (12%), less than one-third of cells being T cells, composed of OKT4+ helper/inducer T cells (18%) and OKT8+ suppressor/cytotoxic T cells (9%). Isolated B cells were responsive to SAC (Cowan type I Staphylococcus aureus) with marked augmentation of IgG production, but not to lipopolysaccharides (LPS). In the presence or absence of pokeweed mitogen (PWM) and protein A, all isotype-specific Ig production was augmented along with increasing ratios of autologous T cells to B cells, whereas in the presence of anti-HLA-DR antibodies the production of IgA and IgM was reduced to a great extent. Finally, in vitro induction of concanavalin A (Con A)-induced suppressor T (Ts) cells was less than that of helper T (Th) cells. Thus, normal adult appendiceal (Ap) lymphocyte reactivity is predominated by helper T (Th) cells; IgA isotype-specific Ig production appears to be largely regulated by the autoreactive Th subset. This responsiveness to exogenous stimuli may play an important role in the gut mucosal immune responsiveness.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ault K. A., Springer T. A. Cross-reaction of a rat-anti-mouse phagocyte-specific monoclonal antibody (anti-Mac-1) with human monocytes and natural killer cells. J Immunol. 1981 Jan;126(1):359–364. [PubMed] [Google Scholar]
  2. Bienenstock J., Befus A. D. Mucosal immunology. Immunology. 1980 Oct;41(2):249–270. [PMC free article] [PubMed] [Google Scholar]
  3. Bockman D. E., Cooper M. D. Pinocytosis by epithelium associated with lymphoid follicles in the bursa of Fabricius, appendix, and Peyer's patches. An electron microscopic study. Am J Anat. 1973 Apr;136(4):455–477. doi: 10.1002/aja.1001360406. [DOI] [PubMed] [Google Scholar]
  4. Christmas S. E., Allan G., Moore M. Naturally cytotoxic tonsillar leukocytes: phenotypic characterization of the effector population. Scand J Immunol. 1985 Jul;22(1):61–71. doi: 10.1111/j.1365-3083.1985.tb01860.x. [DOI] [PubMed] [Google Scholar]
  5. Clayberger C., DeKruyff R. H., Cantor H. T cell regulation of antibody responses: an I-A-specific, autoreactive T cell collaborates with antigen-specific helper T cells to promote IgG responses. J Immunol. 1985 Feb;134(2):691–694. [PubMed] [Google Scholar]
  6. Cooper M. D., Perey D. Y., McKneally M. F., Gabrielsen A. E., Sutherland D. E., Good R. A. A mammalian equivalent of the avian bursa of Fabricius. Lancet. 1966 Jun 25;1(7452):1388–1391. doi: 10.1016/s0140-6736(66)90300-x. [DOI] [PubMed] [Google Scholar]
  7. Falkoff R. M., Peters M., Fauci A. S. T cell enrichment and depletion of human peripheral blood mononuclear cell preparations. Unexpected findings in the study of the functional activities of the separated populations. J Immunol Methods. 1982;50(1):39–49. doi: 10.1016/0022-1759(82)90302-7. [DOI] [PubMed] [Google Scholar]
  8. Finnegan A., Needleman B., Hodes R. J. Activation of B cells by autoreactive T cells: cloned autoreactive T cells activate B cells by two distinct pathways. J Immunol. 1984 Jul;133(1):78–85. [PubMed] [Google Scholar]
  9. Hanaoka M., Nomoto K., Waksman B. H. Appendix and gamma M-antibody formation. I. Immune response and tolerance to bovine gamma globulin in irradiated, appendix-shielded rabbits. J Immunol. 1970 Mar;104(3):616–625. [PubMed] [Google Scholar]
  10. Husband A. J., Gowans J. L. The origin and antigen-dependent distribution of IgA-containing cells in the intestine. J Exp Med. 1978 Nov 1;148(5):1146–1160. doi: 10.1084/jem.148.5.1146. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Kawanishi H., Ozato K., Strober W. The proliferative response of cloned Peyer's patch switch T cells to syngeneic and allogeneic stimuli. J Immunol. 1985 Jun;134(6):3586–3591. [PubMed] [Google Scholar]
  12. Kawanishi H., Saltzman L. E., Strober W. Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. I. T cells derived from Peyer's patches that switch sIgM B cells to sIgA B cells in vitro. J Exp Med. 1983 Feb 1;157(2):433–450. doi: 10.1084/jem.157.2.433. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kawanishi H., Strober W. T cell regulation of IgA immunoglobulin production in gut-associated lymphoid tissues. Mol Immunol. 1983 Sep;20(9):917–930. doi: 10.1016/0161-5890(83)90034-2. [DOI] [PubMed] [Google Scholar]
  14. Lanier L. L., Le A. M., Phillips J. H., Warner N. L., Babcock G. F. Subpopulations of human natural killer cells defined by expression of the Leu-7 (HNK-1) and Leu-11 (NK-15) antigens. J Immunol. 1983 Oct;131(4):1789–1796. [PubMed] [Google Scholar]
  15. Lems-Van Kan P., Verspaget H. W., Peña A. S. ELISA assay for quantitative measurement of human immunoglobulins IgA, IgG, and IgM in nanograms. J Immunol Methods. 1983 Feb 25;57(1-3):51–57. doi: 10.1016/0022-1759(83)90064-9. [DOI] [PubMed] [Google Scholar]
  16. Mage M. G., McHugh L. L., Rothstein T. L. Mouse lymphocytes with and without surface immunoglobulin: preparative scale separation in polystyrene tissue culture dishes coated with specifically purified anti-immunoglobulin. J Immunol Methods. 1977;15(1):47–56. doi: 10.1016/0022-1759(77)90016-3. [DOI] [PubMed] [Google Scholar]
  17. Ozer H., Waksman B. H. Appendix and gamma-M antibody formation. IV. Synergism of appendix and bone marrow cells in early antibody response to sheep erythrocytes. J Immunol. 1970 Sep;105(3):791–792. [PubMed] [Google Scholar]
  18. Phillips J. H., Warner N. L., Lanier L. L. Correlation of biophysical properties and cell surface antigenic profile of Percoll gradient-separated human natural killer cells. Nat Immun Cell Growth Regul. 1983;3(2):73–86. [PubMed] [Google Scholar]
  19. Reinherz E. L., Penta A. C., Hussey R. E., Schlossman S. F. A rapid method for separating functionally intact human T lymphocytes with monoclonal antibodies. Clin Immunol Immunopathol. 1981 Nov;21(2):257–266. doi: 10.1016/0090-1229(81)90214-2. [DOI] [PubMed] [Google Scholar]
  20. Spencer J., Finn T., Isaacson P. G. Gut associated lymphoid tissue: a morphological and immunocytochemical study of the human appendix. Gut. 1985 Jul;26(7):672–679. doi: 10.1136/gut.26.7.672. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Toma V. A., Reteif F. P. Human vermiform appendix. Immunocompetent cell topography and cell-to-cell interactions in situ. J Immunol Methods. 1978;20:333–347. doi: 10.1016/0022-1759(78)90269-7. [DOI] [PubMed] [Google Scholar]

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