Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1987 Jun;61(2):207–213.

The expression of class II MHC gene products by fallopian tube epithelium in pregnancy and throughout the menstrual cycle.

J N Bulmer, U Earl
PMCID: PMC1453383  PMID: 3298023

Abstract

The expression of HLA class II antigens by human fallopian tube epithelium was investigated in ectopic tubal pregnancy, in normal early and full-term intrauterine pregnancy, and during the menstrual cycle. Monoclonal antibodies directed against non-polymorphic (DA6.231, CR3/43, B7/21) and polymorphic (DA6.147, DA6.164, anti-leu-10) determinants of the HLA-D locus were used in a standard indirect immunoperoxidase method on fresh cryostat sections of fallopian tube. In ectopic pregnancy the tube epithelium showed uniform, intense reactivity for DR, DP and DQ. A similar reaction pattern was observed in normal first-trimester pregnancy. At term, most epithelial cells were DR-, DP- and DQ-positive, but a few were DP- and DQ-negative. In fallopian tubes from non-pregnant individuals, a variable number of epithelial cells labelled for DR alpha and DR beta but there was essentially no reactivity for DP or DQ. These results suggest differential regulation of class II MHC gene expression by tube epithelial cells, possibly mediated by hormones and/or a trophoblast product.

Full text

PDF
207

Images in this article

210Figure 1
on p.210
210Figure 2
on p.210
211Figure 3
on p.211

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Basham T. Y., Merigan T. C. Recombinant interferon-gamma increases HLA-DR synthesis and expression. J Immunol. 1983 Apr;130(4):1492–1494. [PubMed] [Google Scholar]
  2. Berrih S., Arenzana-Seisdedos F., Cohen S., Devos R., Charron D., Virelizier J. L. Interferon-gamma modulates HLA class II antigen expression on cultured human thymic epithelial cells. J Immunol. 1985 Aug;135(2):1165–1171. [PubMed] [Google Scholar]
  3. Bottazzo G. F., Dean B. M., McNally J. M., MacKay E. H., Swift P. G., Gamble D. R. In situ characterization of autoimmune phenomena and expression of HLA molecules in the pancreas in diabetic insulitis. N Engl J Med. 1985 Aug 8;313(6):353–360. doi: 10.1056/NEJM198508083130604. [DOI] [PubMed] [Google Scholar]
  4. Bulmer J. N., Sunderland C. A. Immunohistological characterization of lymphoid cell populations in the early human placental bed. Immunology. 1984 Jun;52(2):349–357. [PMC free article] [PubMed] [Google Scholar]
  5. Chen Y. X., Evans R. L., Pollack M. S., Lanier L. L., Phillips J. H., Rousso C., Warner N. L., Brodsky F. M. Characterization and expression of the HLA-DC antigens defined by anti-Leu 10. Hum Immunol. 1984 Aug;10(4):221–235. doi: 10.1016/0198-8859(84)90088-0. [DOI] [PubMed] [Google Scholar]
  6. Daar A. S., Fabre J. W. The membrane antigens of human colorectal cancer cells: demonstration with monoclonal antibodies of heterogeneity within and between tumours and of anomalous expression of HLA-DR. Eur J Cancer Clin Oncol. 1983 Feb;19(2):209–220. doi: 10.1016/0277-5379(83)90419-4. [DOI] [PubMed] [Google Scholar]
  7. Daar A. S., Fuggle S. V., Fabre J. W., Ting A., Morris P. J. The detailed distribution of MHC Class II antigens in normal human organs. Transplantation. 1984 Sep;38(3):293–298. doi: 10.1097/00007890-198409000-00019. [DOI] [PubMed] [Google Scholar]
  8. Dalchau R., Kirkley J., Fabre J. W. Monoclonal antibody to a human leukocyte-specific membrane glycoprotein probably homologous to the leukocyte-common (L-C) antigen of the rat. Eur J Immunol. 1980 Oct;10(10):737–744. doi: 10.1002/eji.1830101003. [DOI] [PubMed] [Google Scholar]
  9. Dimitriu-Bona A., Burmester G. R., Waters S. J., Winchester R. J. Human mononuclear phagocyte differentiation antigens. I. Patterns of antigenic expression on the surface of human monocytes and macrophages defined by monoclonal antibodies. J Immunol. 1983 Jan;130(1):145–152. [PubMed] [Google Scholar]
  10. Earl U., Wells M., Bulmer J. N. Immunohistochemical characterisation of trophoblast antigens and secretory products in ectopic tubal pregnancy. Int J Gynecol Pathol. 1986;5(2):132–142. [PubMed] [Google Scholar]
  11. Earl U., Wells M., Bulmer J. N. The expression of major histocompatibility complex antigens by trophoblast in ectopic tubal pregnancy. J Reprod Immunol. 1985 Aug;8(1):13–24. doi: 10.1016/0165-0378(85)90074-9. [DOI] [PubMed] [Google Scholar]
  12. Edwards J. A., Jones D. B., Evans P. R., Smith J. L. Differential expression of HLA class II antigens on human fetal and adult lymphocytes and macrophages. Immunology. 1985 Jul;55(3):489–500. [PMC free article] [PubMed] [Google Scholar]
  13. Ferguson A., Moore M., Fox H. Expression of MHC products and leucocyte differentiation antigens in gynaecological neoplasms: an immunohistological analysis of the tumour cells and infiltrating leucocytes. Br J Cancer. 1985 Oct;52(4):551–563. doi: 10.1038/bjc.1985.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Fischer A., Sterkers G., Charron D., Durandy A. HLA class II restriction governing cell cooperation between antigen-specific helper T lymphocytes, B lymphocytes and monocytes for in vitro antibody production to influenza virus. Eur J Immunol. 1985 Jun;15(6):620–626. doi: 10.1002/eji.1830150617. [DOI] [PubMed] [Google Scholar]
  15. Flynn A. Stimulation of interleukin-1 production from placental monocytes. Lymphokine Res. 1984;3(1):1–5. [PubMed] [Google Scholar]
  16. Gonwa T. A., Picker L. J., Raff H. V., Goyert S. M., Silver J., Stobo J. D. Antigen-presenting capabilities of human monocytes correlates with their expression of HLA-DS, an Ia determinant distinct from HLA-DR. J Immunol. 1983 Feb;130(2):706–711. [PubMed] [Google Scholar]
  17. Guerry D., 4th, Alexander M. A., Herlyn M. F., Zehngebot L. M., Mitchell K. F., Zmijewski C. M., Lusk E. J. HLA-DR histocompatibility leukocyte antigens permit cultured human melanoma cells from early but not advanced disease to stimulate autologous lymphocytes. J Clin Invest. 1984 Jan;73(1):267–271. doi: 10.1172/JCI111201. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Guy K., Van Heyningen V., Cohen B. B., Deane D. L., Steel C. M. Differential expression and serologically distinct subpopulations of human Ia antigens detected with monoclonal antibodies to Ia alpha and beta chains. Eur J Immunol. 1982 Nov;12(11):942–948. doi: 10.1002/eji.1830121109. [DOI] [PubMed] [Google Scholar]
  19. Hanafusa T., Pujol-Borrell R., Chiovato L., Russell R. C., Doniach D., Bottazzo G. F. Aberrant expression of HLA-DR antigen on thyrocytes in Graves' disease: relevance for autoimmunity. Lancet. 1983 Nov 12;2(8359):1111–1115. doi: 10.1016/s0140-6736(83)90628-1. [DOI] [PubMed] [Google Scholar]
  20. Hirsch M. R., Wietzerbin J., Pierres M., Goridis C. Expression of Ia antigens by cultured astrocytes treated with gamma-interferon. Neurosci Lett. 1983 Oct 31;41(1-2):199–204. doi: 10.1016/0304-3940(83)90247-1. [DOI] [PubMed] [Google Scholar]
  21. Johnson P. M., Bulmer J. N. Uterine gland epithelium in human pregnancy often lacks detectable maternal MHC antigens but does express fetal trophoblast antigens. J Immunol. 1984 Apr;132(4):1608–1610. [PubMed] [Google Scholar]
  22. Klareskog L., Forsum U., Peterson P. A. Hormonal regulation of the expression of Ia antigens on mammary gland epithelium. Eur J Immunol. 1980 Dec;10(12):958–963. doi: 10.1002/eji.1830101212. [DOI] [PubMed] [Google Scholar]
  23. Lampert I. A. Expression of HLA-DR (Ia like) antigen on epidermal keratinocytes in human dermatoses. Clin Exp Immunol. 1984 Jul;57(1):93–100. [PMC free article] [PubMed] [Google Scholar]
  24. Lampert I. A., Suitters A. J., Chisholm P. M. Expression of Ia antigen on epidermal keratinocytes in graft-versus-host disease. Nature. 1981 Sep 10;293(5828):149–150. doi: 10.1038/293149a0. [DOI] [PubMed] [Google Scholar]
  25. Lu C. Y., Changelian P. S., Unanue E. R. Alpha-fetoprotein inhibits macrophage expression of Ia antigens. J Immunol. 1984 Apr;132(4):1722–1727. [PubMed] [Google Scholar]
  26. Morris H., Edwards J., Tiltman A., Emms M. Endometrial lymphoid tissue: an immunohistological study. J Clin Pathol. 1985 Jun;38(6):644–652. doi: 10.1136/jcp.38.6.644. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Natali P. G., Segatto O., Ferrone S., Tosi R., Corte G. Differential tissue distribution and ontogeny of DC-1 and HLA-DR antigens. Immunogenetics. 1984;19(2):109–116. doi: 10.1007/BF00387853. [DOI] [PubMed] [Google Scholar]
  28. Pober J. S., Collins T., Gimbrone M. A., Jr, Cotran R. S., Gitlin J. D., Fiers W., Clayberger C., Krensky A. M., Burakoff S. J., Reiss C. S. Lymphocytes recognize human vascular endothelial and dermal fibroblast Ia antigens induced by recombinant immune interferon. Nature. 1983 Oct 20;305(5936):726–729. doi: 10.1038/305726a0. [DOI] [PubMed] [Google Scholar]
  29. Rognum T. O., Brandtzaeg P., Thorud E. Is heterogeneous expression of HLA-dr antigens and CEA along with DNA-profile variations evidence of phenotypic instability and clonal proliferation in human large bowel carcinomas? Br J Cancer. 1983 Oct;48(4):543–551. doi: 10.1038/bjc.1983.227. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Selby W. S., Janossy G., Mason D. Y., Jewell D. P. Expression of HLA-DR antigens by colonic epithelium in inflammatory bowel disease. Clin Exp Immunol. 1983 Sep;53(3):614–618. [PMC free article] [PubMed] [Google Scholar]
  31. Sone T., Tsukamoto K., Hirayama K., Nishimura Y., Takenouchi T., Aizawa M., Sasazuki T. Two distinct class II molecules encoded by the genes within HLA-DR subregion of HLA-Dw2 and Dw12 can act as stimulating and restriction molecules. J Immunol. 1985 Aug;135(2):1288–1298. [PubMed] [Google Scholar]
  32. Sunderland C. A., Naiem M., Mason D. Y., Redman C. W., Stirrat G. M. The expression of major histocompatibility antigens by human chorionic villi. J Reprod Immunol. 1981 Dec;3(6):323–331. doi: 10.1016/0165-0378(81)90048-6. [DOI] [PubMed] [Google Scholar]
  33. Virelizier J. L., Perez N., Arenzana-Seisdedos F., Devos R. Pure interferon gamma enhances class II HLA antigens on human monocyte cell lines. Eur J Immunol. 1984 Jan;14(1):106–108. doi: 10.1002/eji.1830140120. [DOI] [PubMed] [Google Scholar]
  34. Watson A. J., DeMars R., Trowbridge I. S., Bach F. H. Detection of a novel human class II HLA antigen. 1983 Jul 28-Aug 3Nature. 304(5924):358–361. doi: 10.1038/304358a0. [DOI] [PubMed] [Google Scholar]
  35. Whitwell H. L., Hughes H. P., Moore M., Ahmed A. Expression of major histocompatibility antigens and leucocyte infiltration in benign and malignant human breast disease. Br J Cancer. 1984 Feb;49(2):161–172. doi: 10.1038/bjc.1984.28. [DOI] [PMC free article] [PubMed] [Google Scholar]
  36. van Heyningen V., Guy K., Newman R., Steel C. M. Human MHC class II molecules as differentiation markers. Immunogenetics. 1982;16(5):459–469. doi: 10.1007/BF00372104. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES