Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1987 Aug;61(4):483–488.

Potentiation of delayed-type hypersensitivity by pertussigen or cyclophosphamide with release of different lymphokines.

W A Sewell 1, P A de Moerloose 1, J A Hamilton 1, J W Schrader 1, I R Mackay 1, M A Vadas 1
PMCID: PMC1453421  PMID: 3127325

Abstract

The effects of lymphokine production of two agents known to potentiate delayed-type hypersensitivity (DTH), pertussigen (pertussis toxin) (PT) and cyclophosphamide (CY) have been investigated. These two agents were administered to immunized mice. Subsequently, lymph nodes and spleen cells were exposed to specific antigen in vitro. The resulting culture supernatants were assayed for the presence of lymphokines. Only supernatants of cells from the mice given PT contained appreciable quantities of interferon-gamma (IFN-gamma) and stimulated cells of the monocyte-like WEHI-265 cell line to produce procoagulant activator and plasminogen activator. On the other hand, CY was more effective than PT on the production of interleukin-3 (IL-3). Both adjuvants had small enhancing effects on the production of interleukin-2 (IL-2). With either adjuvant, the cell populations induced had a similarly enhanced capacity to transfer DTH. These results demonstrate that the capacity of cells to transfer DTH does not necessarily correlate with their release of particular lymphokines. The potentiation of DTH by cyclophosphamide did not depend on significantly enhanced generation of IFN-gamma, procoagulant activator, or plasminogen activator. The amount of IFN-gamma in the culture supernatants correlated with their capacity to produce procoagulant activator and plasminogen activator, whereas the amount of IL-2 and IL-3 did not.

Full text

PDF
483

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Clark-Lewis I., Kent S. B., Schrader J. W. Purification to apparent homogeneity of a factor stimulating the growth of multiple lineages of hemopoietic cells. J Biol Chem. 1984 Jun 25;259(12):7488–7494. [PubMed] [Google Scholar]
  2. Colvin R. B., Dvorak H. F. Role of the clotting system in cell-mediated hypersensitivity. II. Kinetics of fibrinogen/fibrin accumulation and vascular permeability changes in tuberculin and cutaneous basophil hypersensitivity reactions. J Immunol. 1975 Jan;114(1 Pt 2):377–387. [PubMed] [Google Scholar]
  3. Crapper R. M., Vairo G., Hamilton J. A., Clark-Lewis I., Schrader J. W. Stimulation of bone marrow-derived and peritoneal macrophages by a T lymphocyte-derived hemopoietic growth factor, persisting cell-stimulating factor. Blood. 1985 Oct;66(4):859–865. [PubMed] [Google Scholar]
  4. Diamantstein T., Klos M., Hahn H., Kaufmann S. H. Direct in vitro evidence for different susceptibilities to 4-hydroperoxycyclophosphamide of antigen-primed T cells regulating humoral and cell-mediated immune responses to sheep erythrocytes: a possible explanation for the inverse action of cyclophosphamide on humoral and cell-mediated immune responses. J Immunol. 1981 May;126(5):1717–1719. [PubMed] [Google Scholar]
  5. Geczy C. L., Hopper K. E. A mechanism of migration inhibition in delayed-type hypersensitivity reactions. II. Lymphokines promote procoagulant activity of macrophages in vitro. J Immunol. 1981 Mar;126(3):1059–1065. [PubMed] [Google Scholar]
  6. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  7. Kelso A., Glasebrook A. L. Secretion of interleukin 2, macrophage-activating factor, interferon, and colony-stimulating factor by alloreactive T lymphocyte clones. J Immunol. 1984 Jun;132(6):2924–2931. [PubMed] [Google Scholar]
  8. Kong A. S., Morse S. I. The in vitro effects of Bordetella pertussis lymphocytosis-promoting factor on murine lymphocytes. I. Proliferative response. J Exp Med. 1977 Jan 1;145(1):151–162. doi: 10.1084/jem.145.1.151. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Miller J. F., Vadas M. A., Whitelaw A., Gamble J. H-2 gene complex restricts transfer of delayed-type hypersensitivity in mice. Proc Natl Acad Sci U S A. 1975 Dec;72(12):5095–5098. doi: 10.1073/pnas.72.12.5095. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Morse S. I., Riester S. K. Studies on the leukocytosis and lymphocytosis induced by Bordetella pertussis. II. The effect of pertussis vaccine on the thoracic duct lymph and lymphocytes of mice. J Exp Med. 1967 Apr 1;125(4):619–628. doi: 10.1084/jem.125.4.619. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Munoz J. J., Bernard C. C., Mackay I. R. Elicitation of experimental allergic encephalomyelitis (EAE) in mice with the aid of pertussigen. Cell Immunol. 1984 Jan;83(1):92–100. doi: 10.1016/0008-8749(84)90228-4. [DOI] [PubMed] [Google Scholar]
  12. Nathan C. F., Murray H. W., Wiebe M. E., Rubin B. Y. Identification of interferon-gamma as the lymphokine that activates human macrophage oxidative metabolism and antimicrobial activity. J Exp Med. 1983 Sep 1;158(3):670–689. doi: 10.1084/jem.158.3.670. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Nogueira N., Gordon S., Cohn Z. Trypanosoma cruzi: the immunological induction of macrophage plasminogen activator requires thymus-derived lymphocytes. J Exp Med. 1977 Jul 1;146(1):172–183. doi: 10.1084/jem.146.1.172. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Pace J. L., Russell S. W., Torres B. A., Johnson H. M., Gray P. W. Recombinant mouse gamma interferon induces the priming step in macrophage activation for tumor cell killing. J Immunol. 1983 May;130(5):2011–2013. [PubMed] [Google Scholar]
  15. Rubin B. Y., Bartal A. H., Anderson S. L., Millet S. K., Hirshaut Y., Feit C. The anticellular and protein-inducing activities of human gamma interferon preparations are mediated by the interferon. J Immunol. 1983 Mar;130(3):1019–1020. [PubMed] [Google Scholar]
  16. Sanderson C. J., Strath M., Warren D. J., O'Garra A., Kirkwood T. B. The production of lymphokines by primary alloreactive T-cell clones: a co-ordinate analysis of 233 clones in seven lymphokine assays. Immunology. 1985 Dec;56(4):575–584. [PMC free article] [PubMed] [Google Scholar]
  17. Sewell W. A., Munoz J. J., Scollay R., Vadas M. A. Studies on the mechanism of the enhancement of delayed-type hypersensitivity by pertussigen. J Immunol. 1984 Oct;133(4):1716–1722. [PubMed] [Google Scholar]
  18. Sewell W. A., Munoz J. J., Vadas M. A. Enhancement of the intensity, persistence, and passive transfer of delayed-type hypersensitivity lesions by pertussigen in mice. J Exp Med. 1983 Jun 1;157(6):2087–2096. doi: 10.1084/jem.157.6.2087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Sewell W. A., de Moerloose P. A., McKimm-Breschkin J. L., Vadas M. A. Pertussigen enhances antigen-driven interferon-gamma production by sensitized lymphoid cells. Cell Immunol. 1986 Feb;97(2):238–247. doi: 10.1016/0008-8749(86)90394-1. [DOI] [PubMed] [Google Scholar]
  20. Steeg P. S., Moore R. N., Johnson H. M., Oppenheim J. J. Regulation of murine macrophage Ia antigen expression by a lymphokine with immune interferon activity. J Exp Med. 1982 Dec 1;156(6):1780–1793. doi: 10.1084/jem.156.6.1780. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Turk J. L., Parker D., Poulter L. W. Functional aspects of the selective depletion of lymphoid tissue by cyclophosphamide. Immunology. 1972 Oct;23(4):493–501. [PMC free article] [PubMed] [Google Scholar]
  22. Wong G. H., Clark-Lewis I., McKimm-Breschkin L., Harris A. W., Schrader J. W. Interferon-gamma induces enhanced expression of Ia and H-2 antigens on B lymphoid, macrophage, and myeloid cell lines. J Immunol. 1983 Aug;131(2):788–793. [PubMed] [Google Scholar]
  23. de Moerloose P. A., Hamilton J. A., Sewell W. A., Vadas M. A., Mackay I. R. Pertussigen in vivo enhances antigen-specific production in vitro of lymphokine that stimulates macrophage procoagulant activity and plasminogen activator. J Immunol. 1986 Dec 1;137(11):3528–3533. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES