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. 1987 Aug;61(4):515–520.

Circulating T-cell populations during mercuric chloride-induced nephritis in the Brown Norway rat.

C Bowman 1, C Green 1, L Borysiewicz 1, C M Lockwood 1
PMCID: PMC1453442  PMID: 3502119

Abstract

Fluorescence-activated cell sorter analysis was used to study the peripheral lymphocyte populations during mercuric chloride (HgCl2)-induced autoimmune nephritis in the Brown Norway (BN) rat. Sequential studies showed a transient loss of T cells from peripheral blood attributable to decreases in the percentage of T-helper cells. In addition, there was a decrease in the percentage of T-cytotoxic/suppressor cells prior to the appearance of circulating anti-GBM antibodies, followed by elevated levels of T-suppressor cells during down-regulation of the response. This method may allow closer inspection of the events linking changes in T-cell populations and induction and termination of an autoimmune response.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bowman C., Mason D. W., Pusey C. D., Lockwood C. M. Autoregulation of autoantibody synthesis in mercuric chloride nephritis in the Brown Norway rat. I. A role for T suppressor cells. Eur J Immunol. 1984 May;14(5):464–470. doi: 10.1002/eji.1830140515. [DOI] [PubMed] [Google Scholar]
  2. Bowman C., Peters D. K., Lockwood C. M. Anti-glomerular basement membrane autoantibodies in the Brown Norway rat: detection by a solid-phase radioimmunoassay. J Immunol Methods. 1983 Jul 29;61(3):325–333. doi: 10.1016/0022-1759(83)90227-2. [DOI] [PubMed] [Google Scholar]
  3. Brideau R. J., Carter P. B., McMaster W. R., Mason D. W., Williams A. F. Two subsets of rat T lymphocytes defined with monoclonal antibodies. Eur J Immunol. 1980 Aug;10(8):609–615. doi: 10.1002/eji.1830100807. [DOI] [PubMed] [Google Scholar]
  4. Chatenoud L., Bach M. A. Abnormalities of T-cell subsets in glomerulonephritis and systemic lupus erythematosus. Kidney Int. 1981 Aug;20(2):267–274. doi: 10.1038/ki.1981.130. [DOI] [PubMed] [Google Scholar]
  5. Craig J. C., Hawkins S. A., Swallow M. W., Lyttle J. A., Patterson V. H., Merrett J. D., Haire M. Subsets of T lymphocytes in relation to T lymphocyte function in multiple sclerosis. Clin Exp Immunol. 1985 Sep;61(3):548–555. [PMC free article] [PubMed] [Google Scholar]
  6. Dallman M. J., Thomas M. L., Green J. R. MRC OX-19: a monoclonal antibody that labels rat T lymphocytes and augments in vitro proliferative responses. Eur J Immunol. 1984 Mar;14(3):260–267. doi: 10.1002/eji.1830140311. [DOI] [PubMed] [Google Scholar]
  7. Druet P., Druet E., Potdevin F., Sapin C. Immune type glomerulonephritis induced by HgCl2 in the Brown Norway rat. Ann Immunol (Paris) 1978 Oct-Dec;129 100(6):777–792. [PubMed] [Google Scholar]
  8. Duke O., Panayi G. S., Janossy G., Poulter L. W., Tidman N. Analysis of T cell subsets in the peripheral blood and synovial fluid of patients with rheumatoid arthritis by means of monoclonal antibodies. Ann Rheum Dis. 1983 Aug;42(4):357–361. doi: 10.1136/ard.42.4.357. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hauser S. L., Bahn A. K., Che M., Gilles F., Weiner H. L. Redistribution of Lyt-bearing T cells in acute murine experimental allergic encephalomyelitis: selective migration of Lyt-1 cells to the central nervous system is associated with a transient depletion of Lyt-1 cells in peripheral blood. J Immunol. 1984 Dec;133(6):3037–3042. [PubMed] [Google Scholar]
  10. Hirsch F., Couderc J., Sapin C., Fournie G., Druet P. Polyclonal effect of HgCl2 in the rat, its possible role in an experimental autoimmune disease. Eur J Immunol. 1982 Jul;12(7):620–625. doi: 10.1002/eji.1830120716. [DOI] [PubMed] [Google Scholar]
  11. Huddlestone J. R., Oldstone M. B. T suppressor (TG) lymphocytes fluctuate in parallel with changes in the clinical course of patients with multiple sclerosis. J Immunol. 1979 Oct;123(4):1615–1618. [PubMed] [Google Scholar]
  12. Hunt S. V., Fowler M. H. A repopulation assay for B and T lymphocyte stem cells employing radiation chimaeras. Cell Tissue Kinet. 1981 Jul;14(4):445–464. doi: 10.1111/j.1365-2184.1981.tb00551.x. [DOI] [PubMed] [Google Scholar]
  13. Lewis D. E., Giorgi J. V., Warner N. L. Flow cytometry analysis of T cells and continuous T-cell lines from autoimmune MRL/l mice. Nature. 1981 Jan 22;289(5795):298–300. doi: 10.1038/289298a0. [DOI] [PubMed] [Google Scholar]
  14. Miller K. B., Schwartz R. S. Autoimmunity and suppressor T lymphocytes. Adv Intern Med. 1982;27:281–313. [PubMed] [Google Scholar]
  15. Okita N., Row V. V., Volpe R. Suppressor T-lymphocyte deficiency in Graves' disease and Hashimoto's thyroiditis. J Clin Endocrinol Metab. 1981 Mar;52(3):528–533. doi: 10.1210/jcem-52-3-528. [DOI] [PubMed] [Google Scholar]
  16. Pelletier L., Pasquier R., Hirsch F., Sapin C., Druet P. In vivo self-reactivity of mononuclear cells to T cells and macrophages exposed to HgCl2. Eur J Immunol. 1985 May;15(5):460–465. doi: 10.1002/eji.1830150509. [DOI] [PubMed] [Google Scholar]
  17. Pontesilli O., Chase H. P., Carotenuto P., Herberger M. J., Hayward A. R. T-lymphocyte subpopulations in insulin-dependent (type I) diabetes mellitus. Clin Exp Immunol. 1986 Jan;63(1):68–72. [PMC free article] [PubMed] [Google Scholar]
  18. Rosenberg J. S., Feldman J. D. Activation of rat B lymphocytes. I. Characterization of anti-immunoglobulin responses and isotype density of rat B cells. J Immunol. 1982 Feb;128(2):651–655. [PubMed] [Google Scholar]
  19. Sapin C., Druet E., Druet P. Induction of anti-glomerular basement membrane antibodies in the Brown-Norway rat by mercuric chloride. Clin Exp Immunol. 1977 Apr;28(1):173–179. [PMC free article] [PubMed] [Google Scholar]
  20. Walls R. S., Basten A., Leuchars E., Davies A. J. Mechanisms for eosinophilic and neutrophilic leucocytoses. Br Med J. 1971 Jul 17;3(5767):157–159. doi: 10.1136/bmj.3.5767.157. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Yale J. F., Marliss E. B. Altered immunity and diabetes in the BB rat. Clin Exp Immunol. 1984 Jul;57(1):1–11. [PMC free article] [PubMed] [Google Scholar]

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