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. 1986 Jul;58(3):445–452.

Anti-idiotypic sera against monoclonal anti-progesterone antibodies: production in rabbits and rats and characterization of specificity.

M J Taussig, N Brown, S Ellis, A Holliman, D Peat, N Richardson, R B Heap, A Feinstein
PMCID: PMC1453462  PMID: 3488262

Abstract

Antisera were raised in rabbits and rats against three mouse monoclonal anti-progesterone IgG1 antibodies. Anti-idiotypic antibodies were isolated from rabbit sera by successive passage over immunoadsorbent columns of normal mouse Ig and the specific immunizing monoclonal, with elution from the latter. Radioimmunoassays for anti-idiotype and free idiotype were established, enabling detection of idiotype in sera of mice immunized with progesterone-BSA conjugate. Binding of rabbit anti-idiotype to anti-progesterone monoclonals was partially inhibitable by free progesterone-hemisuccinate or progesterone-ovalbumin conjugate. While showing considerable specificity for their respective inducing monoclonals, the anti-idiotypes also cross-reacted in varying degrees with the other anti-progesterone monoclonals, demonstrating the presence of IdI and IdX determinants. The patterns of cross-reactivity showed some correlation with the relative isoelectric points and combining-site specificities of the anti-progesterones. The specificities of rat and rabbit anti-idiotypes were similar, but not identical.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Capra J. D., Kehoe J. M. Hypervariable regions, idiotypy, and the antibody-combining site. Adv Immunol. 1975;20:1–40. doi: 10.1016/s0065-2776(08)60205-9. [DOI] [PubMed] [Google Scholar]
  2. Kunkel H. G., Mannik M., Williams R. C. Individual Antigenic Specificity of Isolated Antibodies. Science. 1963 Jun 14;140(3572):1218–1219. doi: 10.1126/science.140.3572.1218. [DOI] [PubMed] [Google Scholar]
  3. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  4. Lieberman R., Potter M., Humphrey W., Jr, Mushinski E. B., Vrana M. Multiple individual and cross-specific indiotypes on 13 levan-binding myeloma proteins of BALB/c mice. J Exp Med. 1975 Jul 1;142(1):106–119. doi: 10.1084/jem.142.1.106. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mason D. W., Williams A. F. The kinetics of antibody binding to membrane antigens in solution and at the cell surface. Biochem J. 1980 Apr 1;187(1):1–20. doi: 10.1042/bj1870001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Milstein C. P., Richardson N. E., Dieverson E. V., Feinstein A. Interchain disulphide bridges of mouse immunoglobulin M. Biochem J. 1975 Dec;151(3):615–624. doi: 10.1042/bj1510615. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Potter M. Antigen-binding myeloma proteins of mice. Adv Immunol. 1977;25:141–211. [PubMed] [Google Scholar]
  8. Rider V., McRae A., Heap R. B., Feinstein A. Passive immunization against progesterone inhibits endometrial sensitization in pseudopregnant mice and has antifertility effects in pregnant mice which are reversible by steroid treatment. J Endocrinol. 1985 Jan;104(1):153–158. doi: 10.1677/joe.0.1040153. [DOI] [PubMed] [Google Scholar]
  9. Rudikoff S. Immunoglobulin structure--function correlates: antigen binding and idiotypes. Contemp Top Mol Immunol. 1983;9:169–209. doi: 10.1007/978-1-4684-4517-6_6. [DOI] [PubMed] [Google Scholar]
  10. Strosberg A. D. Anti-idiotype and anti-hormone receptor antibodies. Springer Semin Immunopathol. 1983;6(1):67–78. doi: 10.1007/BF01857367. [DOI] [PubMed] [Google Scholar]
  11. Symons D. B., Clarkson C. A., Binns R. M. Antibody response to dinitrophenyl hapten by foetal, neonatal and young pigs. Immunology. 1983 Apr;48(4):703–711. [PMC free article] [PubMed] [Google Scholar]
  12. Wang M. Y., Rider V., Heap R. B., Feinstein A. Action of anti-progesterone monoclonal antibody in blocking pregnancy after postcoital administration in mice. J Endocrinol. 1984 Apr;101(1):95–100. doi: 10.1677/joe.0.1010095. [DOI] [PubMed] [Google Scholar]
  13. Wright L. J., Feinstein A., Heap R. B., Saunders J. C., Bennett R. C., Wang M. Y. Progesterone monoclonal antibody blocks pregnancy in mice. Nature. 1982 Feb 4;295(5848):415–417. doi: 10.1038/295415a0. [DOI] [PubMed] [Google Scholar]

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