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. 1986 Jul;58(3):411–416.

Proliferation and transepithelial migration of mucosal mast cells in interstitial cystitis.

F Aldenborg, M Fall, L Enerbäck
PMCID: PMC1453481  PMID: 3733146

Abstract

The distribution and abundance of mast cells, as well as their fixation, staining and ultrastructural properties, were studied in the urinary bladders of 16 patients with interstitial cystitis (IC) and in 14 normal subjects. Tissues were fixed in both standard formaldehyde solution and a special fixative, IFAA, optimized for the preservation of mucosal mast cells. An expansion of two distinct mast cell populations was observed in IC. One of these, comprising formaldehyde-sensitive cells, was found only in the mucosa underlying lesions of IC. They were most numerous in the lamina propria but were also frequent in the epithelial layer as well as in the bladder washings, indicating a migratory capacity for these cells. The other mast cell population was visualized equally well irrespective of fixation and staining procedure. In control subjects, such cells were found both in the lamina propria and detrusor muscle, but not in the epithelium nor in bladder washings. In lesions of IC they were increased in the detrusor muscle only. Both types of mast cell contained granules with the highly characteristic lamellar arrays and scrolls, distinguishing human mast cell granules from those of blood basophils. The proliferation and intraepithelial distribution of mucosal mast cells are unusual findings, but prominent features of helminth responses and human mucosal allergic reactions. These findings thus suggest that the mucosal mast cell-IgE system may be involved in the pathogenesis and/or aetiology of IC.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allenmark S., Bergström S., Enerbäck L. A selective postcolumn o-phthalaldehyde-derivatization system for the determination of histamine in biological material by high-performance liquid chromatography. Anal Biochem. 1985 Jan;144(1):98–103. doi: 10.1016/0003-2697(85)90089-2. [DOI] [PubMed] [Google Scholar]
  2. Befus A. D., Pearce F. L., Gauldie J., Horsewood P., Bienenstock J. Mucosal mast cells. I. Isolation and functional characteristics of rat intestinal mast cells. J Immunol. 1982 Jun;128(6):2475–2480. [PubMed] [Google Scholar]
  3. Brinkman G. L. The mast cell in normal human bronchus and lung. J Ultrastruct Res. 1968 Apr;23(1):115–123. doi: 10.1016/s0022-5320(68)80035-8. [DOI] [PubMed] [Google Scholar]
  4. Enerbäck L., Kolset S. O., Kusche M., Hjerpe A., Lindahl U. Glycosaminoglycans in rat mucosal mast cells. Biochem J. 1985 Apr 15;227(2):661–668. doi: 10.1042/bj2270661. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Enerbäck L., Pipkorn U., Granerus G. Intraepithelial migration of nasal mucosal mast cells in hay fever. Int Arch Allergy Appl Immunol. 1986;80(1):44–51. doi: 10.1159/000234024. [DOI] [PubMed] [Google Scholar]
  6. Fall M., Johansson S. L., Vahlne A. A clinicopathological and virological study of interstitial cystitis. J Urol. 1985 May;133(5):771–773. doi: 10.1016/s0022-5347(17)49221-4. [DOI] [PubMed] [Google Scholar]
  7. Hanash K. A., Pool T. L. Interstitial and hemorrhagic cystitis: viral, bacterial and fungal studies. J Urol. 1970 Nov;104(5):705–706. doi: 10.1016/s0022-5347(17)61815-9. [DOI] [PubMed] [Google Scholar]
  8. Hedelin H. H., Mårdh P. A., Brorson J. E., Fall M., Møller B. R., Pettersson K. G. Mycoplasma hominis and interstitial cystitis. Sex Transm Dis. 1983 Oct-Dec;10(4 Suppl):327–330. [PubMed] [Google Scholar]
  9. Jacobo E., Stamler F. W., Culp D. A. Interstitial cystitis followed by total cystectomy. Urology. 1974 Apr;3(4):481–485. doi: 10.1016/s0090-4295(74)80169-x. [DOI] [PubMed] [Google Scholar]
  10. Jarrett E. E., Miller H. R. Production and activities of IgE in helminth infection. Prog Allergy. 1982;31:178–233. [PubMed] [Google Scholar]
  11. KIRKMAN H. A comparative morphological and cytochemical study of globule leucocytes (Schollenleukocyten) of the urinary tract and of possibly related cells. Am J Anat. 1950 Jan;86(1):91-131, illust. doi: 10.1002/aja.1000860105. [DOI] [PubMed] [Google Scholar]
  12. Kastrup J., Hald T., Larsen S., Nielsen V. G. Histamine content and mast cell count of detrusor muscle in patients with interstitial cystitis and other types of chronic cystitis. Br J Urol. 1983 Oct;55(5):495–500. doi: 10.1111/j.1464-410x.1983.tb03356.x. [DOI] [PubMed] [Google Scholar]
  13. Larsen S., Thompson S. A., Hald T., Barnard R. J., Gilpin C. J., Dixon J. S., Gosling J. A. Mast cells in interstitial cystitis. Br J Urol. 1982 Jun;54(3):283–286. doi: 10.1111/j.1464-410x.1982.tb06976.x. [DOI] [PubMed] [Google Scholar]
  14. Lose G., Frandsen B., Højensgård J. C., Jespersen J., Astrup T. Chronic interstitial cystitis: increased levels of eosinophil cationic protein in serum and urine and an ameliorating effect of subcutaneous heparin. Scand J Urol Nephrol. 1983;17(2):159–161. doi: 10.3109/00365598309180161. [DOI] [PubMed] [Google Scholar]
  15. Mattila J. Vascular immunopathology in interstitial cystitis. Clin Immunol Immunopathol. 1982 Jun;23(3):648–655. doi: 10.1016/0090-1229(82)90327-0. [DOI] [PubMed] [Google Scholar]
  16. Miller H. R., Jarrett W. F. Immune reactions in mucous membranes. I. Intestinal mast cell response during helminth expulsion in the rat. Immunology. 1971 Mar;20(3):277–288. [PMC free article] [PubMed] [Google Scholar]
  17. Olafsson J. H., Roupe G., Enerbäck L. Dermal mast cells in mastocytosis: fixation, distribution and quantitation. Acta Derm Venereol. 1986;66(1):16–22. [PubMed] [Google Scholar]
  18. Oravisto K. J. Interstitial cystitis as an autoimmune disease. A review. Eur Urol. 1980;6(1):10–13. doi: 10.1159/000473278. [DOI] [PubMed] [Google Scholar]
  19. Pearce F. L., Befus A. D., Gauldie J., Bienenstock J. Mucosal mast cells. II. Effects of anti-allergic compounds on histamine secretion by isolated intestinal mast cells. J Immunol. 1982 Jun;128(6):2481–2486. [PubMed] [Google Scholar]
  20. REBUCK J. W., HODSON J. M., PRIEST R. J., BARTH C. L. Basophilic granulocytes in inflammatory tissues of man. Ann N Y Acad Sci. 1963 Feb 26;103:409–426. doi: 10.1111/j.1749-6632.1963.tb53712.x. [DOI] [PubMed] [Google Scholar]
  21. Rosin R. D., Griffiths T., Sofras F., James D. C., Edwards L. Interstitial cystitis. Br J Urol. 1979 Dec;51(6):524–527. doi: 10.1111/j.1464-410x.1979.tb03593.x. [DOI] [PubMed] [Google Scholar]
  22. Ruitenberg E. J., Gustowska L., Elgersma A., Ruitenberg H. M. Effect of fixation on the light microscopical visualization of mast cells in the mucosa and connective tissue of the human duodenum. Int Arch Allergy Appl Immunol. 1982;67(3):233–238. doi: 10.1159/000233024. [DOI] [PubMed] [Google Scholar]
  23. Smith B. H., Dehner L. P. Chronic ulcerating interstitial cystitis (Hunner's ulcer). A study of 28 cases. Arch Pathol. 1972 Jan;93(1):76–81. [PubMed] [Google Scholar]
  24. Wingren U., Enerbäck L. Mucosal mast cells of the rat intestine: a re-evaluation of fixation and staining properties, with special reference to protein blocking and solubility of the granular glycosaminoglycan. Histochem J. 1983 Jun;15(6):571–582. doi: 10.1007/BF01954148. [DOI] [PubMed] [Google Scholar]

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