Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Feb;54(2):289–295.

Enhancement of immunity against RSV-induced sarcomas by generation of hapten-reactive helper T lymphocytes.

P M Comoglio, M Prat, S Bretti
PMCID: PMC1453510  PMID: 2578431

Abstract

Previous work from this laboratory has shown that preimmunization of syngeneic hosts with Rous sarcoma virus (RSV)-transformed cells elicits a strong immune response against the growth of transplantable RSV sarcomas, mediated by T lymphocytes expressing the surface phenotype of helper cell precursors (Prat, Di Renzo & Comoglio, 1983). This paper shows that anti-tumour immunity may be elicited in tumour-bearing animals by triggering an experimentally pre-amplified T-helper cell population at the site of tumour growth. Mice were treated with cyclophosphamide (which inactivates suppressor T cells) followed by skin sensitization to trinitrochlorobenzene (TNCB) according to a protocol that has been shown to induce an appreciably amplified generation of trinitrophenyl (TNP)-reactive helper T cells (Fujiwara et al., 1984). Five weeks after TNCB painting, mice were transplanted s.c. with a lethal dose of RSV-induced syngeneic sarcoma cells; the injection at the tumour site of TNCB induced the regression of the tumour in mice in which the TNP-helper cell population has been amplified, but not in controls, including those injected with a non-related hapten or sensitized to TNCB without inactivation of suppressors.

Full text

PDF
289

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ATTIA M. A., DEOME K. B., WEISS D. W. IMMUNOLOGY OF SPONTANEOUS MAMMARY CARCINOMAS IN MICE. II. RESISTANCE TO A RAPIDLY AND A SLOWLY DEVELOPING TUMOR. Cancer Res. 1965 May;25:451–457. [PubMed] [Google Scholar]
  2. Aupoix Michèle C., Biquard J. M., Cachard A. Cell surface antigen induced by avian tumor viruses in hamster cells transformed by a temperature-sensitive mutant of Rous sarcoma virus. Int J Cancer. 1974 Nov 15;14(5):611–616. doi: 10.1002/ijc.2910140507. [DOI] [PubMed] [Google Scholar]
  3. Bauer H. Virion and tumor cell antigens of C-type rna tumor viruses. Adv Cancer Res. 1974;20:275–341. doi: 10.1016/s0065-230x(08)60112-8. [DOI] [PubMed] [Google Scholar]
  4. Biddison W. E., Palmer J. C., Alexander M. A., Cowan E. P., Manson L. A. Characterization and specificity of murine anti-tumor cytotoxic effector cells within an ascitic tumor. J Immunol. 1977 Jun;118(6):2243–2253. [PubMed] [Google Scholar]
  5. Bubeník J., Baresovà M., Micheel B., Sovová V., el-Ridi R., Keclíková M. Rous sarcomas induced by heterotransplantation of virogenic rat tumour cells in mice. Int J Cancer. 1972 Nov;10(3):527–538. doi: 10.1002/ijc.2910100311. [DOI] [PubMed] [Google Scholar]
  6. Cerottini J. C., Brunner K. T. Cell-mediated cytotoxicity, allograft rejection, and tumor immunity. Adv Immunol. 1974;18:67–132. doi: 10.1016/s0065-2776(08)60308-9. [DOI] [PubMed] [Google Scholar]
  7. Cheever M. A., Greenberg P. D., Fefer A. Tumor neutralization, immunotherapy, and chemoimmunotherapy of a Friend leukemia with cells secondarily sensitized in vitro: II. Comparison of cells cultured with and without tumor to noncultured immune cells. J Immunol. 1978 Dec;121(6):2220–2227. [PubMed] [Google Scholar]
  8. Comoglio P. M., Prat M., Bertini M. A virus-induced non-virion antigen specific for transformation at the surface of RSV-transformed fibroblasts. Nature. 1978 Jun 1;273(5661):381–383. doi: 10.1038/273381a0. [DOI] [PubMed] [Google Scholar]
  9. Comoglio P. M., Tarone G., Prat M., Bertini M. Studies on the outer surface of normal and RSV-transformed BHK fibroblast plasma membrane. Exp Cell Res. 1975 Jul;93(2):402–410. doi: 10.1016/0014-4827(75)90466-8. [DOI] [PubMed] [Google Scholar]
  10. Di Renzo M. F., Bretti S. Characterization of stable spontaneous metastatic variant lines of RSV-transformed mouse fibroblasts. Int J Cancer. 1982 Dec 15;30(6):751–757. doi: 10.1002/ijc.2910300612. [DOI] [PubMed] [Google Scholar]
  11. Di Renzo M. F., Doneda L., Larizza L., Comoglio P. M. Metastatic clones selected from an RSV-induced mouse sarcoma share a common marker chromosome. Int J Cancer. 1983 Apr 15;31(4):455–461. doi: 10.1002/ijc.2910310410. [DOI] [PubMed] [Google Scholar]
  12. Fernandez-Cruz E., Gilman S. C., Feldman J. D. Immunotherapy of a chemically-induced sarcoma in rats: characterization of the effector T cell subset and nature of suppression. J Immunol. 1982 Mar;128(3):1112–1117. [PubMed] [Google Scholar]
  13. Ferracini R., Prat M., Comoglio P. M. Dissection of the antigenic determinants expressed on the cell surface of RSV-transformed fibroblasts by monoclonal antibodies. Int J Cancer. 1982 Apr 15;29(4):477–481. doi: 10.1002/ijc.2910290419. [DOI] [PubMed] [Google Scholar]
  14. Fujiwara H., Hamaoka T., Shearer G. M., Yamamoto H., Terry W. D. The augmentation of in vitro and in vivo tumor-specific T cell-mediated immunity by amplifier T lymphocytes. J Immunol. 1980 Feb;124(2):863–869. [PubMed] [Google Scholar]
  15. Fujiwara H., Levy R. B., Shearer G. M., Terry W. D. Studies on in vivo priming of the TNP-reactive cytotoxic effector cell system. I. Comparison of the effects of intravenous inoculation with TNP-conjugated cells on the development of contact sensitivity and cell-mediated lympholysis. J Immunol. 1979 Jul;123(1):423–425. [PubMed] [Google Scholar]
  16. Fujiwara H., Moriyama Y., Suda T., Tsuchida T., Shearer G. M., Hamaoka T. Enhanced TNP-reactive helper T cell activity and its utilization in the induction of amplified tumor immunity that results in tumor regression. J Immunol. 1984 Mar;132(3):1571–1577. [PubMed] [Google Scholar]
  17. Gorczynski R. M. Evidence for in vivo protection against murine-sarcoma virus-induced tumors by T lymphocytes from immune animals. J Immunol. 1974 Feb;112(2):533–539. [PubMed] [Google Scholar]
  18. Greenberg P. D., Cheever M. A., Fefer A. Eradication of disseminated murine leukemia by chemoimmunotherapy with cyclophosphamide and adoptively transferred immune syngeneic Lyt-1+2- lymphocytes. J Exp Med. 1981 Sep 1;154(3):952–963. doi: 10.1084/jem.154.3.952. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. JONSSON N., SJOEGREN H. O. FURTHER STUDIES ON SPECIFIC TRANSPLANTATION ANTIGENS IN ROUS SARCOMA OF MICE. J Exp Med. 1965 Aug 1;122:403–421. doi: 10.1084/jem.122.2.403. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kurth R., Bauer H. Cell-surface antigens induced by avian RNA tumor viruses: detection by a cytotoxic microassay. Virology. 1972 Feb;47(2):426–433. doi: 10.1016/0042-6822(72)90278-4. [DOI] [PubMed] [Google Scholar]
  21. Kurth R., Macpherson I. A. Avian cell transformation and the expression of avian sarcoma virus-specific tumour antigens. Nature. 1976 Nov 18;264(5583):261–263. doi: 10.1038/264261a0. [DOI] [PubMed] [Google Scholar]
  22. Landt M., Boltz S. C., Butler L. G. Alkaline phosphatase: affinity chromatography and inhibition by phosphonic acids. Biochemistry. 1978 Mar 7;17(5):915–919. doi: 10.1021/bi00598a027. [DOI] [PubMed] [Google Scholar]
  23. Leclerc J. C., Cantor H. T cell-mediated immunity to oncornavirus-induced tumors. I. Ly phenotype of precursor and effector cytolytic T lymphocytes. J Immunol. 1980 Feb;124(2):846–850. [PubMed] [Google Scholar]
  24. Leclerc J. C., Cantor H. T cell-mediated immunity to oncornavirus-induced tumors. II. Ability of different T cell sets to prevent tumor growth in vivo. J Immunol. 1980 Feb;124(2):851–854. [PubMed] [Google Scholar]
  25. Parsons S. J., Riley S. C., Mullen E. E., Brock E. J., Benjamin D. C., Kuehl W. M., Parsons J. T. Immune response to the src gene product in mice bearing tumors induced by injection of avian sarcoma virus-transformed mouse cells. J Virol. 1979 Oct;32(1):40–46. doi: 10.1128/jvi.32.1.40-46.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Prat M., Di Renzo M. F., Comoglio P. M. Characterization of T lymphocytes mediating in vivo protection against RSV-induced murine sarcomas. Int J Cancer. 1983 Jun 15;31(6):757–764. doi: 10.1002/ijc.2910310614. [DOI] [PubMed] [Google Scholar]
  27. Prat M., Tarone G., Comoglio P. M. Mouse fibroblasts transformed by Rous sarcoma virus express a virus-specific non-virion transplantation antigen. Int J Cancer. 1981 Jun 15;27(6):797–805. doi: 10.1002/ijc.2910270611. [DOI] [PubMed] [Google Scholar]
  28. Prat M., Tato F., Tarone G., Comoglio P. M. Target antigens for antibodies and complement at the cell surface of RSV-transformed fibroblasts. Immunology. 1980 Feb;39(2):179–185. [PMC free article] [PubMed] [Google Scholar]
  29. Prat M., Tatò F., Tarone G., Comoglio P. M. Interaction between cellular and viral genes in the expression of the RSV-induced transformation-specific cell-surface antigen VCSA. Int J Cancer. 1980 Mar 15;25(3):355–362. doi: 10.1002/ijc.2910250309. [DOI] [PubMed] [Google Scholar]
  30. Röllinghoff M., Starzinski-Powitz A., Pfizenmaier K., Wagner H. Cyclophosphamide-sensitive T lymphocytes suppress the in vivo generation of antigen-specific cytotoxic T lymphocytes. J Exp Med. 1977 Feb 1;145(2):455–459. doi: 10.1084/jem.145.2.455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Schmitt-Verhulst A. M., Pettinelli C. B., Henkart P. A., Lunney J. K., Shearer G. M. H-2-restricted cytotoxic effectors generated in vitro by the addition of trinitrophenyl-conjugated soluble proteins. J Exp Med. 1978 Feb 1;147(2):352–368. doi: 10.1084/jem.147.2.352. [DOI] [PMC free article] [PubMed] [Google Scholar]
  32. Schwartz A., Askenase P. W., Gershon R. K. Regulation of delayed-type hypersensitivity reactions by cyclophosphamide-sensitive T cells. J Immunol. 1978 Oct;121(4):1573–1577. [PubMed] [Google Scholar]
  33. Sy M. S., Miller S. D., Claman H. N. Immune suppression with supraoptimal doses of antigen in contact sensitivity. I. Demonstration of suppressor cells and their sensitivity to cyclophosphamide. J Immunol. 1977 Jul;119(1):240–244. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES