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. 1985 Mar;54(3):505–512.

Influence of serotonin on the immune response.

J C Jackson, R J Cross, R F Walker, W R Markesbery, W H Brooks, T L Roszman
PMCID: PMC1453513  PMID: 3156091

Abstract

The present study investigates the influence of pharmacological agents known to regulate biosynthesis of the neurotransmitter, serotonin (5-hydroxytryptamine, 5-HT) on the primary antibody response to sheep red blood cells (SRBC) in the CBA mouse. Systemic administration of 5-HT (4-100 mg/kg) or its precursor, 5-hydroxytryptophan (5-HTP, 50-400 mg/kg), 30-60 min before immunization resulted in dose-dependent suppression of both the IgM and IgG plaque-forming cell (PFC) response to SRBC. Conversely, para-chlorophenylalanine (PCPA, 250 mg/kg), which inhibits the rate-limiting enzyme (tryptophan hydroxylase) in 5-HT biosynthesis, markedly enhanced IgM and IgG antibody production when injected 48 hr prior to antigen. Effects of these drugs on immune processes appeared independent of observed changes in plasma corticosterone levels. Further, immune function was preserved following selective depletion of brain serotonin through intracisternal injection of the neurotoxin 5,7-dihydroxytryptamine (5,7-DHT) in mice pretreated with desmethylimipramine (DMI). Thus, immunomodulation by serotonin appears to be mediated via peripheral mechanism(s).

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abraham A. D., Buga G. '3H-testosterone distribution and binding in rat thymus cells in vivo'. Mol Cell Biochem. 1976 Dec 10;13(3):157–163. doi: 10.1007/BF01731778. [DOI] [PubMed] [Google Scholar]
  2. Besedovsky H., Sorkin E., Felix D., Haas H. Hypothalamic changes during the immune response. Eur J Immunol. 1977 May;7(5):323–325. doi: 10.1002/eji.1830070516. [DOI] [PubMed] [Google Scholar]
  3. Besedovsky H., del Rey A., Sorkin E., Da Prada M., Burri R., Honegger C. The immune response evokes changes in brain noradrenergic neurons. Science. 1983 Aug 5;221(4610):564–566. doi: 10.1126/science.6867729. [DOI] [PubMed] [Google Scholar]
  4. Bhathena S. J., Louie J., Schechter G. P., Redman R. S., Wahl L., Recant L. Identification of human mononuclear leukocytes bearing receptors for somatostatin and glucagon. Diabetes. 1981 Feb;30(2):127–131. doi: 10.2337/diab.30.2.127. [DOI] [PubMed] [Google Scholar]
  5. Björklund A., Baumgarten H. G., Nobin A. Chemical lesioning of central monoamine axons by means of 5,6-dihydroxytryptamine and 5,7-dihydroxytryptamine. Adv Biochem Psychopharmacol. 1974;10:13–33. [PubMed] [Google Scholar]
  6. Björklund A., Baumgarten H. G., Rensch A. 5,7-Dihydroxytryptamine: improvement of its selectivity for serotonin neurons in the CNS by pretreatment with desipramine. J Neurochem. 1975 Apr;24(4):833–835. [PubMed] [Google Scholar]
  7. Bliznakov E. G. Serotonin and its precursors as modulators of the immunological responsiveness in mice. J Med. 1980;11(2-3):81–105. [PubMed] [Google Scholar]
  8. Cross R. J., Markesbery W. R., Brooks W. H., Roszman T. L. Hypothalamic-immune interactions: neuromodulation of natural killer activity by lesioning of the anterior hypothalamus. Immunology. 1984 Feb;51(2):399–405. [PMC free article] [PubMed] [Google Scholar]
  9. Csaba G., Sudár F., Dobozy O. Triiodothyronine receptors in lymphocytes of newborn and adult rats. Horm Metab Res. 1977 Nov;9(6):499–501. doi: 10.1055/s-0028-1093509. [DOI] [PubMed] [Google Scholar]
  10. Cunningham A. J., Szenberg A. Further improvements in the plaque technique for detecting single antibody-forming cells. Immunology. 1968 Apr;14(4):599–600. [PMC free article] [PubMed] [Google Scholar]
  11. Devoino L., Eliseeva L., Eremina O., Idova G., Cheido M. 5-Hydroxytryptophan effect on the development of the immune response: IgM and IgG antibodies and rosette formation in primary and secondary responses. Eur J Immunol. 1976 Jun;5(6):394–399. doi: 10.1002/eji.1830050608. [DOI] [PubMed] [Google Scholar]
  12. Elliott L. H., Roszman T. L. Antigenic selection of IgG precursor subpopulations. J Immunol. 1975 Aug;115(2):495–501. [PubMed] [Google Scholar]
  13. Eremina O. F., Devoino L. V. Produktsiia gumoral'nykh antitel u krolikove pri razrushenii iadra srednemozgovogo shva. Biull Eksp Biol Med. 1973 Feb;74(2):58–60. [PubMed] [Google Scholar]
  14. Fleminger S., Jenner P., Marsden C. D. Are dopamine receptors present on human lymphocytes? J Pharm Pharmacol. 1982 Oct;34(10):658–663. doi: 10.1111/j.2042-7158.1982.tb04696.x. [DOI] [PubMed] [Google Scholar]
  15. Goodwin J. S., Wiik A., Lewis M., Bankhurst A. D., Williams R. C., Jr High-affinity binding sites for prostaglandin E on human lymphocytes. Cell Immunol. 1979 Mar 1;43(1):150–159. doi: 10.1016/0008-8749(79)90158-8. [DOI] [PubMed] [Google Scholar]
  16. Hazum E., Chang K. J., Cuatrecasas P. Specific nonopiate receptors for beta-endorphin. Science. 1979 Sep 7;205(4410):1033–1035. doi: 10.1126/science.224457. [DOI] [PubMed] [Google Scholar]
  17. Homo F., Picard F., Durant S., Gagne D., Simon J., Dardenne M., Duval D. Glucocorticoid receptors and their functions in lymphocytes. J Steroid Biochem. 1980 Jan;12:433–443. doi: 10.1016/0022-4731(80)90304-0. [DOI] [PubMed] [Google Scholar]
  18. Keller S. E., Stein M., Camerino M. S., Schleifer S. J., Sherman J. Suppression of lymphocyte stimulation by anterior hypothalamic lesions in the guinea pig. Cell Immunol. 1980 Jul 1;52(2):334–340. doi: 10.1016/0008-8749(80)90354-8. [DOI] [PubMed] [Google Scholar]
  19. Koe B. K., Weissman A. p-Chlorophenylalanine: a specific depletor of brain serotonin. J Pharmacol Exp Ther. 1966 Dec;154(3):499–516. [PubMed] [Google Scholar]
  20. LUPARELLO T. J., STEIN M., PARK C. D. EFFECT OF HYPOTHALAMIC LESIONS ON RAT ANAPHYLAXIS. Am J Physiol. 1964 Oct;207:911–914. doi: 10.1152/ajplegacy.1964.207.4.911. [DOI] [PubMed] [Google Scholar]
  21. Le Fur G., Phan T., Canton T., Tur C., Uzan A. Evidence for a coupling between dopaminergic receptors and phospholipid methylation in mouse B lymphocytes. Life Sci. 1981 Dec 28;29(26):2737–2749. doi: 10.1016/0024-3205(81)90533-6. [DOI] [PubMed] [Google Scholar]
  22. Lesniak M. A., Gorden P., Roth J. Reactivity of non-primate growth hormones and prolactins with human growth hormone receptors on cultured human lymphocytes. J Clin Endocrinol Metab. 1977 May;44(5):838–849. doi: 10.1210/jcem-44-5-838. [DOI] [PubMed] [Google Scholar]
  23. Loveland B. E., Jarrott B., McKenzie I. F. The detection of beta-adrenoceptors on murine lymphocytes. Int J Immunopharmacol. 1981;3(1):45–55. doi: 10.1016/0192-0561(81)90044-8. [DOI] [PubMed] [Google Scholar]
  24. Maśliński W., Grabczewska E., Ryzewski J. Acetylcholine receptors of rat lymphocytes. Biochim Biophys Acta. 1980 Dec 1;633(2):269–273. doi: 10.1016/0304-4165(80)90412-2. [DOI] [PubMed] [Google Scholar]
  25. Ogden B. E., Hill H. R. Histamine regulates lymphocyte mitogenic responses through activation of specific H1 and H2 histamine receptors. Immunology. 1980 Sep;41(1):107–114. [PMC free article] [PubMed] [Google Scholar]
  26. Pochet R., Delespesse G., Gausset P. W., Collet H. Distribution of beta-adrenergic receptors on human lymphocyte subpopulations. Clin Exp Immunol. 1979 Dec;38(3):578–584. [PMC free article] [PubMed] [Google Scholar]
  27. Richman D. P., Antel J. P., Burns J. B., Arnason B. G. Nicotinic acetylcholine receptor on human lymphocytes. Ann N Y Acad Sci. 1981;377:427–435. doi: 10.1111/j.1749-6632.1981.tb33750.x. [DOI] [PubMed] [Google Scholar]
  28. Roszman T. L., Cross R. J., Brooks W. H., Markesbery W. R. Hypothalamic-immune interactions. II. The effect of hypothalamic lesions on the ability of adherent spleen cells to limit lymphocyte blastogenesis. Immunology. 1982 Apr;45(4):737–742. [PMC free article] [PubMed] [Google Scholar]
  29. Saavedra J. M., Brownstein M., Axelrod J. A specific and sensitive enzymatic-isotopic microassay for serotonin in tissues. J Pharmacol Exp Ther. 1973 Sep;186(3):508–515. [PubMed] [Google Scholar]
  30. Smith K. A., Crabtree G. R., Kennedy S. J., Munck A. U. Glucocorticoid receptors and glucocorticoid sensitivity of mitogen stimulated and unstimulated human lymphocytes. Nature. 1977 Jun 9;267(5611):523–526. doi: 10.1038/267523a0. [DOI] [PubMed] [Google Scholar]
  31. Tyrey L., Nalbandov A. V. Influence of anterior hypothalamic lesions on circulating antibody titers in the rat. Am J Physiol. 1972 Jan;222(1):179–185. doi: 10.1152/ajplegacy.1972.222.1.179. [DOI] [PubMed] [Google Scholar]
  32. Vetoshkin A. V., Fomenko A. M., Zozulia A. A. Serotoninovye retseptory limfotsitov: radioretseptornoe issledovanie. Biull Eksp Biol Med. 1982 Jul;94(7):52–53. [PubMed] [Google Scholar]
  33. Watanabe Y., Lai R. T., Yoshida H. The beta-adrenoreceptor in human lymphocytes. Clin Exp Pharmacol Physiol. 1981 May-Jun;8(3):273–276. doi: 10.1111/j.1440-1681.1981.tb00159.x. [DOI] [PubMed] [Google Scholar]
  34. Wurtman R. J., Fernstrom J. D. Control of brain neurotransmitter synthesis by precursor availability and nutritional state. Biochem Pharmacol. 1976 Aug 1;25(15):1691–1696. doi: 10.1016/0006-2952(76)90400-7. [DOI] [PubMed] [Google Scholar]

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