Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Mar;54(3):497–504.

Effects of multiple oral dosing on IgE synthesis in mice: oral sensitization by albumin extracts from seeds of Jack fruit (Artocarpus integrifolia) containing lectins.

N Restum-Miguel, A Prouvost-Danon
PMCID: PMC1453520  PMID: 3972438

Abstract

The IgE antibody response was studied in DBA/2 mice; the mice were pretreated orally with albumin extracts from seeds of Jack fruit (Jackalbumin) and subsequently immunized subcutaneously with Jackalbumin mixed with ovalbumin (OA) and a synthetic adjuvant, muramyl dipeptide (MDP). The allergenicity of Jackalbumin was evaluated by its capacity to induce a specific IgE response which was measured by passive cutaneous anaphylaxis (PCA) and by degranulation of washed peritoneal mast cells following antigen challenge (Jackalbumin or OA). Antibody against the crude extracts and anti-lectin (FIISP) IgE responses were also tested by PCA. After being fed eight doses of 1 mg Jackalbumin, DBA/2 mice became immunized: i.e. specific IgE antibody responses were observed and the peritoneal mast cells became sensitized. An increase in IgE response was verified in mice that were pre-fed and subsequently immunized. The results indicated that: the albumin extracts from Jack seeds, containing lectins, can be allergenic by the oral route; multiple oral doses with these extracts can induce an enhancement of the IgE response on subsequent subcutaneous immunization; antigenically, Jackalbumin does not seem to cross-react with OA; the lectins contained in the albumin fraction from Artocarpus seeds were also shown to be allergenic; the IgE titres showed an inverse correlation to the degree of purification of the lectin used for PCA challenge.

Full text

PDF
497

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. André C., Bazin H., Heremans J. F. Influence of repeated administration of antigen by the oral route on specific antibody-producing cells in the mouse spleen. Digestion. 1973;9(2):166–175. doi: 10.1159/000197442. [DOI] [PubMed] [Google Scholar]
  2. Audibert F., Chédid L., Lefrancier P., Choay J. Distinctive adjuvanticity of synthetic analogs of mycobacterial water-soluble components. Cell Immunol. 1976 Feb;21(2):243–249. doi: 10.1016/0008-8749(76)90053-8. [DOI] [PubMed] [Google Scholar]
  3. Barth R. F., Singla O. Differential effects of concanavalin A on T helper dependent and independent antibody responses. Cell Immunol. 1973 Oct;9(1):96–103. doi: 10.1016/0008-8749(73)90171-8. [DOI] [PubMed] [Google Scholar]
  4. Bazin H., Platteau B. Production of circulating reaginic (IgE) antibodies by oral administration of ovalbumin to rats. Immunology. 1976 May;30(5):679–684. [PMC free article] [PubMed] [Google Scholar]
  5. Crabbé P. A., Nash D. R., Bazin H., Eyssen D. V., Heremans J. F. Antibodies of the IgA type in intestinal plasma cells of germfree mice after oral or parenteral immunization with ferritin. J Exp Med. 1969 Oct 1;130(4):723–744. doi: 10.1084/jem.130.4.723. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Egan H. S., Reeder W. J., Ekstedt R. D. Effect of concanavalin A in vivo in suppressing the antibody response in mice. J Immunol. 1974 Jan;112(1):63–69. [PubMed] [Google Scholar]
  7. Ellouz F., Adam A., Ciorbaru R., Lederer E. Minimal structural requirements for adjuvant activity of bacterial peptidoglycan derivatives. Biochem Biophys Res Commun. 1974 Aug 19;59(4):1317–1325. doi: 10.1016/0006-291x(74)90458-6. [DOI] [PubMed] [Google Scholar]
  8. Elves M. W. Suppression of antibody production by phytohaemagglutinin. Nature. 1967 Feb 4;213(5075):495–496. doi: 10.1038/213495a0. [DOI] [PubMed] [Google Scholar]
  9. Falchuk K. R., Walker W. A., Perrotto J. L., Isselbacher K. J. Effect of vitamin A on the systemic and local antibody responses to intragastrically administered bovine serum albumin. Infect Immun. 1977 Aug;17(2):361–365. doi: 10.1128/iai.17.2.361-365.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Gollapudi V. S., Kind L. S. Enhancement of reaginic antibody formation in the mouse by concanavalin A. Int Arch Allergy Appl Immunol. 1975;48(1):94–100. doi: 10.1159/000231295. [DOI] [PubMed] [Google Scholar]
  11. Hanson D. G., Vaz N. M., Maia L. C., Hornbrook M. M., Lynch J. M., Roy C. A. Inhibition of specific immune responses by feeding protein antigens. Int Arch Allergy Appl Immunol. 1977;55(1-6):526–532. doi: 10.1159/000231966. [DOI] [PubMed] [Google Scholar]
  12. Howard J. G., Mitchison N. A. Immunological tolerance. Prog Allergy. 1975;18:43–96. doi: 10.1159/000395256. [DOI] [PubMed] [Google Scholar]
  13. Jarrett E. E., Haig D. M., McDougall W., McNulty E. Rat IgE production. II. Primary and booster reaginic antibody responses following intradermal or oral immunization. Immunology. 1976 May;30(5):671–677. [PMC free article] [PubMed] [Google Scholar]
  14. Krause R. M. The time has come. J Allergy Clin Immunol. 1977 Jul;60(1):1–7. doi: 10.1016/0091-6749(77)90076-8. [DOI] [PubMed] [Google Scholar]
  15. Lawrence D. A., Schell R. F. Differential effects of concanavalin A and phytohemagglutinin on murine immunity. Suppression and enhancement of humoral immunity. Cell Immunol. 1977 Jun 1;31(1):155–162. doi: 10.1016/0008-8749(77)90014-4. [DOI] [PubMed] [Google Scholar]
  16. Leclerc C., Löwy I., Chedid L. Influence of MDP and of some analogous synthetic glycopeptides on the in vitro mouse spleen cell viability and immune response to sheep erythrocytes. Cell Immunol. 1978 Jul;38(2):286–293. doi: 10.1016/0008-8749(78)90059-x. [DOI] [PubMed] [Google Scholar]
  17. Mitchell G. F., Clarke A. E. Allergenicity of concanavalin A in mice. Int Arch Allergy Appl Immunol. 1979;58(4):391–401. doi: 10.1159/000232218. [DOI] [PubMed] [Google Scholar]
  18. Mota I., Wong D. Homologous and heterologous passive cutaneous anaphylactic activity of mouse antisera during the course of immunization. Life Sci. 1969 Aug 15;8(16):813–820. doi: 10.1016/0024-3205(69)90099-x. [DOI] [PubMed] [Google Scholar]
  19. Ngan J., Kind L. S. Suppressor T cells for IgE and IgG in Peyer's patches of mice made tolerant by the oral administration of ovalbumin. J Immunol. 1978 Mar;120(3):861–865. [PubMed] [Google Scholar]
  20. Ohkuni H., Norose Y., Ohta M., Hayama M., Kimura Y., Tsujimoto M., Kotani S., Shiba T., Kusumoto S., Yokogawa K. Adjuvant activities in production of reaginic antibody by bacterial cell wall peptidoglycan or synthetic N-acetylmuramyl dipeptides in mice. Infect Immun. 1979 May;24(2):313–318. doi: 10.1128/iai.24.2.313-318.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Prouvost-Danon A., Lima M. S., Javierre M. Q. Active anaphylactic reaction in mouse peritoneal mast cells in vitro. Life Sci. 1966 Feb;5(4):289–297. doi: 10.1016/0024-3205(66)90013-0. [DOI] [PubMed] [Google Scholar]
  22. Richman L. K., Chiller J. M., Brown W. R., Hanson D. G., Vaz N. M. Enterically induced immunologic tolerance. I. Induction of suppressor T lymphoyctes by intragastric administration of soluble proteins. J Immunol. 1978 Dec;121(6):2429–2434. [PubMed] [Google Scholar]
  23. Romball C. G., Weigle W. O. The enhancing effect of mitogens on the in vivo immune response of rabbits. J Immunol. 1975 Aug;115(2):556–560. [PubMed] [Google Scholar]
  24. Rothberg R. M., Kraft S. C., Farr R. S. Similarities between rabbit antibodies produced following ingestion of bovine serum albumin and following parenteral immunization. J Immunol. 1967 Feb;98(2):386–395. [PubMed] [Google Scholar]
  25. Schell R. F., Lawrence D. A. Differential effects of concanavalin A and phytohemagglutinin on murine immunity. Suppression and enhancement of cell-mediated immunity. Cell Immunol. 1977 Jun 1;31(1):142–154. doi: 10.1016/0008-8749(77)90013-2. [DOI] [PubMed] [Google Scholar]
  26. Stokes C. R., Swarbrick E. T. Induction of tolerance after oral feeding of soluble protein antigen [proceedings]. Biochem Soc Trans. 1977;5(5):1573–1573. doi: 10.1042/bst0051573. [DOI] [PubMed] [Google Scholar]
  27. Tada T. Regulation of reaginic antibody formation in animals. Prog Allergy. 1975;19:122–194. [PubMed] [Google Scholar]
  28. Taylor C. E., Stashak P. W., Caldes G., Prescott B., Fowlkes B. J., Baker P. J. Lectin-induced modulation of the antibody response to type III pneumococcal polysaccharide. Cell Immunol. 1984 Jan;83(1):26–33. doi: 10.1016/0008-8749(84)90221-1. [DOI] [PubMed] [Google Scholar]
  29. Thomas H. C., Ferguson A., McLennan J. G., Mason D. K. Food antibodies in oral disease: a study of serum antibodies to food proteins in aphthous ulceration and other oral diseases. J Clin Pathol. 1973 May;26(5):371–374. doi: 10.1136/jcp.26.5.371. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES