Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Mar;54(3):533–540.

Dexamethasone suppresses concanavalin A-induced production of chemotactic lymphokines by releasing a soluble factor from splenic T lymphocytes.

M Hirashima, K Sakata, K Tashiro, T Yoshimura, H Hayashi
PMCID: PMC1453531  PMID: 3871733

Abstract

Treatment of guinea-pig spleen cells with glucocorticoids, such as dexamethasone (DEX), reduces concanavalin A (Con A)-induced production of chemotactic lymphokines (CLK), such as eosinophil chemotactic factor and macrophage chemotactic factor. The decreased CLK production is not caused by a direct effect of DEX on the spleen cells producing CLK, because Con A-induced CLK production is suppressed when the cells are cultured together with cell-free culture supernatants of the spleen cells which had been pretreated with DEX. A soluble suppressive factor, termed CLK-SF, with a MW of about 20,000, seems to be responsible for the suppression of both CLK production. CLK-SF is produced from DEX-treated T lymphocytes. CLK-SF probably exerts a critical role in the early stage of CLK production. In contrast, CLK-SF fails to inhibit Con A-induced lymphocyte proliferation, although DEX itself suppresses lymphocyte proliferation. This suggests that DEX suppresses Con A-induced CLK production by a different mechanism from that for lymphocyte proliferation.

Full text

PDF
533

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Balow J. E., Rosenthal A. S. Glucocorticoid suppression of macrophage migration inhibitory factor. J Exp Med. 1973 Apr 1;137(4):1031–1041. doi: 10.1084/jem.137.4.1031. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Foley J. E., Jeffries M., Munck A. Glucocorticoid effects on incorporation of lipid and protein precursors into rat thymus cell fractions. J Steroid Biochem. 1980 Jan;12:231–243. doi: 10.1016/0022-4731(80)90273-3. [DOI] [PubMed] [Google Scholar]
  3. Gillis S., Crabtree G. R., Smith K. A. Glucocorticoid-induced inhibition of T cell growth factor production. I. The effect on mitogen-induced lymphocyte proliferation. J Immunol. 1979 Oct;123(4):1624–1631. [PubMed] [Google Scholar]
  4. Gillis S., Crabtree G. R., Smith K. A. Glucocorticoid-induced inhibition of T cell growth factor production. II. The effect on the in vitro generation of cytolytic T cells. J Immunol. 1979 Oct;123(4):1632–1638. [PubMed] [Google Scholar]
  5. Hirashima M., Hayashi H. The mediation of tissue eosinophilia in hypersensitivity reaction. I. Isolation of two different chemotactic factors from DNP-Ascaris extract-induced skin lesion in guinea-pig. Immunology. 1976 Feb;30(2):203–212. [PMC free article] [PubMed] [Google Scholar]
  6. Hirashima M., Honda M., Hayashi H. The mediation of tissue eosinophilia in hypersensitivity reactions. II. Separation of a delayed eosinophil chemotactic factor from macrophage chemotactic factors. Immunology. 1976 Aug;31(2):263–271. [PMC free article] [PubMed] [Google Scholar]
  7. Julius M. H., Simpson E., Herzenberg L. A. A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol. 1973 Oct;3(10):645–649. doi: 10.1002/eji.1830031011. [DOI] [PubMed] [Google Scholar]
  8. NOWELL P. C. Inhibition of human leukocyte mitosis by prednisolone in vitro. Cancer Res. 1961 Dec;21:1518–1521. [PubMed] [Google Scholar]
  9. Neifeld J. P., Tormey D. C. Effects of steroid hormones on phytohemagglutinin-stimulated human peripheral blood lymphocytes. Transplantation. 1979 May;27(5):309–314. doi: 10.1097/00007890-197905000-00004. [DOI] [PubMed] [Google Scholar]
  10. Smith K. A., Crabtree G. R., Kennedy S. J., Munck A. U. Glucocorticoid receptors and glucocorticoid sensitivity of mitogen stimulated and unstimulated human lymphocytes. Nature. 1977 Jun 9;267(5611):523–526. doi: 10.1038/267523a0. [DOI] [PubMed] [Google Scholar]
  11. Snyder D. S., Unanue E. R. Corticosteroids inhibit murine macrophage Ia expression and interleukin 1 production. J Immunol. 1982 Nov;129(5):1803–1805. [PubMed] [Google Scholar]
  12. Stosić-Grujicić S., Simić M. M. Modulation of interleukin 1 production by activated macrophages: in vitro action of hydrocortisone, colchicine, and cytochalasin B. Cell Immunol. 1982 May 15;69(2):235–247. doi: 10.1016/0008-8749(82)90070-3. [DOI] [PubMed] [Google Scholar]
  13. Wahl S. M., Altman L. C., Rosenstreich D. L. Inhibition of in vitro lymphokine synthesis by glucocorticosteroids. J Immunol. 1975 Aug;115(2):476–481. [PubMed] [Google Scholar]
  14. Yodoi J., Hirashima M., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. II. Glycoprotein nature and source of IgE-potentiating factor. J Immunol. 1980 Oct;125(4):1436–1441. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES