Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Apr;54(4):677–683.

Activation and proliferation signals in mouse B cells. V.A. comparison of the effects of intact (IgG) and F (ab')2 anti-mu or anti-delta antibodies.

G G Klaus, M K Bijsterbosch, R M Parkhouse
PMCID: PMC1453550  PMID: 2984110

Abstract

This study compares the effects of soluble intact (IgG) and F(ab')2 rabbit anti-mu and anti-delta antibodies on mouse B cells. The results show that while the F(ab')2 antibodies to both isotypes induce polyclonal B cell proliferation, the IgG antibodies are not mitogenic, but rather inhibit DNA synthesis induced by the homologous F(ab')2 fragments. Furthermore, intact anti-mu antibodies inhibit mitogenesis induced by F(ab')2 anti-delta, and vice versa. However, the intact antibodies to both isotypes promote early changes characteristic of B-cell activation, namely, increased Ia antigen expression, and priming for a facilitated proliferative response to F(ab')2 anti-Ig. In addition, F(ab')2 anti-mu and anti-delta both induce the breakdown of phosphatidylinositol phospholipids in B cells, an early consequence of Ig receptor cross-linking which may be involved in the induction of cell growth. These results therefore indicate that stimulating mature B cells via IgM or IgD receptors produces indistinguishable early effects, and that cross-linking Fc and surface Ig receptors by intact anti-Ig generates a dominant inhibitory signal, regardless of which isotype is involved.

Full text

PDF
677

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersson J., Bullock W. W., Melchers F. Inhibition of mitogenic stimulation of mouse lymphocytes by anti-mouse immunoglobulin antibodies. I. Mode of action. Eur J Immunol. 1974 Nov;4(11):715–722. doi: 10.1002/eji.1830041103. [DOI] [PubMed] [Google Scholar]
  2. BLIGH E. G., DYER W. J. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959 Aug;37(8):911–917. doi: 10.1139/o59-099. [DOI] [PubMed] [Google Scholar]
  3. Berridge M. J. Inositol trisphosphate and diacylglycerol as second messengers. Biochem J. 1984 Jun 1;220(2):345–360. doi: 10.1042/bj2200345. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bourgois A., Abney E. R., Parkhouse R. M. Mouse immunoglobulin receptors on lymphocytes: identification of IgM and IgD molecules by tryptic cleavage and a postulated role for cell surface IgD. Eur J Immunol. 1977 Apr;7(4):210–213. doi: 10.1002/eji.1830070404. [DOI] [PubMed] [Google Scholar]
  5. Cambier J. C., Ligler F. S., Uhr J. W., Kettman J. R., Vitetta E. S. Blocking of primary in vitro antibody responses to thymus-independent and thymus-dependent antigens with antiserum specific for IgM or IgD. Proc Natl Acad Sci U S A. 1978 Jan;75(1):432–435. doi: 10.1073/pnas.75.1.432. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Chayen A., Parkhouse R. M. B cell subpopulations in the mouse: analysis with monoclonal antibodies NIM-R2 and NIM-R3. Eur J Immunol. 1982 Sep;12(9):725–732. doi: 10.1002/eji.1830120906. [DOI] [PubMed] [Google Scholar]
  7. Dresser D. W., Parkhouse R. M. The effect of the parenteral administration of a rabbit anti-(mouse)-IgD serum on the immune response of mice to sheep erythrocytes. Immunology. 1978 Dec;35(6):1027–1036. [PMC free article] [PubMed] [Google Scholar]
  8. Havran W. L., DiGiusto D. L., Cambier J. C. mIgM:mIgD ratios on B cells: mean mIgD expression exceeds mIgM by 10-fold on most splenic B cells. J Immunol. 1984 Apr;132(4):1712–1716. [PubMed] [Google Scholar]
  9. Hawrylowicz C. M., Keeler K. D., Klaus G. G. Activation and proliferation signals in mouse B cells. I. A comparison of the capacity of anti-Ig antibodies or phorbol myristic acetate to activate B cells from CBA/N or normal mice into G1. Eur J Immunol. 1984 Mar;14(3):244–250. doi: 10.1002/eji.1830140308. [DOI] [PubMed] [Google Scholar]
  10. Klaus G. G., Hawrylowicz C. M., Holman M., Keeler K. D. Activation and proliferation signals in mouse B cells. III. Intact (IGG) anti-immunoglobulin antibodies activate B cells but inhibit induction of DNA synthesis. Immunology. 1984 Dec;53(4):693–701. [PMC free article] [PubMed] [Google Scholar]
  11. Mond J. J., Seghal E., Kung J., Finkelman F. D. Increased expression of I-region-associated antigen (Ia) on B cells after cross-linking of surface immunoglobulin. J Immunol. 1981 Sep;127(3):881–888. [PubMed] [Google Scholar]
  12. Phillips N. E., Parker D. C. Cross-linking of B lymphocyte Fc gamma receptors and membrane immunoglobulin inhibits anti-immunoglobulin-induced blastogenesis. J Immunol. 1984 Feb;132(2):627–632. [PubMed] [Google Scholar]
  13. ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. I. A UNIQUE MYELOMA PROTEIN. J Exp Med. 1965 Jan 1;121:171–184. doi: 10.1084/jem.121.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Scribner D. J., Weiner H. L., Moorhead J. W. Anti-immunoglobulin stimulation of murine lymphocytes. V. Age-related decline in Fc receptor-mediated immunoregulation. J Immunol. 1978 Jul;121(1):377–382. [PubMed] [Google Scholar]
  15. Sidman C. L., Unanue E. R. Control of B-lymphocyte function. I. Inactivation of mitogenesis by interactions with surface immunoglobulin and Fc-receptor molecules. J Exp Med. 1976 Oct 1;144(4):882–896. doi: 10.1084/jem.144.4.882. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sieckmann D. G., Finkelman F. D., Thompson C. B., Scher I. Activation of mouse lymphocytes by anti-immunoglobulin. IV. Stimulation with soluble heterologous anti-delta antibodies. Cell Immunol. 1984 Apr 15;85(1):1–14. doi: 10.1016/0008-8749(84)90272-7. [DOI] [PubMed] [Google Scholar]
  17. Sieckmann D. G. The use of anti-immunoglobulins to induce a signal for cell division in B lymphocytes via their membrane IgM and IgD. Immunol Rev. 1980;52:181–210. doi: 10.1111/j.1600-065x.1980.tb00335.x. [DOI] [PubMed] [Google Scholar]
  18. Tony H. P., Schimpl A. Stimulation of murine B cells with anti-Ig antibodies: dominance of a negative signal mediated by the Fc receptor. Eur J Immunol. 1980 Sep;10(9):726–729. doi: 10.1002/eji.1830100914. [DOI] [PubMed] [Google Scholar]
  19. Vitetta E. S., Uhr J. W. Immunoglobulin-receptors revisited. Science. 1975 Sep 19;189(4207):964–969. doi: 10.1126/science.1083069. [DOI] [PubMed] [Google Scholar]
  20. Vitetta E., Puré E., Isakson P., Buck L., Uhr J. The activation of murine B cells: the role of surface immunoglobulins. Immunol Rev. 1980;52:211–231. doi: 10.1111/j.1600-065x.1980.tb00336.x. [DOI] [PubMed] [Google Scholar]
  21. Zitron I. M., Clevinger B. L. Regulation of murine B cells through surface immunoglobulin. I. Monoclonal anti-delta antibody that induces allotype-specific proliferation. J Exp Med. 1980 Nov 1;152(5):1135–1146. doi: 10.1084/jem.152.5.1135. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Zitron I. M., Mosier D. E., Paul W. E. The role of surface IgD in the response to thymic-independent antigens. J Exp Med. 1977 Dec 1;146(6):1707–1718. doi: 10.1084/jem.146.6.1707. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES