Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Apr;54(4):655–663.

Resistance against Brugia malayi microfilariae induced by a monoclonal antibody which promotes killing by macrophages and recognizes surface antigen(s).

A Aggarwal, W Cuna, A Haque, C Dissous, A Capron
PMCID: PMC1453564  PMID: 3884492

Abstract

Several monoclonal antibodies were produced following the immunization of mice with infective larvae of Brugia malayi. One of these gives a positive fluorescence reaction on the surface of B. malayi microfilariae and this particular monoclonal antibody (IgM isotype) was able to mediate mouse peritoneal macrophage adherence to, and killing of, B. malayi microfilariae in vitro. Adherence and killing were enhanced by fresh normal mouse serum, suggesting a role for complement. When the same monoclonal antibody was passively transferred to mice harbouring microfilariae in their circulation, a complete clearance of microfilariae was observed in 70% of the animals. This monoclonal antibody was able to recognize antigenic determinants (of 110,000 MW) present on the surface of B. malayi microfilariae by radioimmunoprecipitation.

Full text

PDF
655

Images in this article

661Figure 2
on p.661

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aiyar S., Zaman V., Ha C. S. Mechanism of destruction of Brugia malayi microfilariae in vitro: the role of antibody and leucocytes. Acta Trop. 1982 Sep;39(3):225–236. [PubMed] [Google Scholar]
  2. Ash L. R. Chronic Brugia pahangi and Brugia malayi infections in Meriones unguiculatus. J Parasitol. 1973 Jun;59(3):442–447. [PubMed] [Google Scholar]
  3. Canlas M., Wadee A., Lamontagne L., Piessens W. F. A monoclonal antibody to surface antigens on microfilariae of Brugia malayi reduces microfilaremia in infected jirds. Am J Trop Med Hyg. 1984 May;33(3):420–424. doi: 10.4269/ajtmh.1984.33.420. [DOI] [PubMed] [Google Scholar]
  4. El-Sadr W. M., Aikawa M., Greene B. M. In vitro immune mechanisms associated with clearance of microfilariae of Dirofilaria immitis. J Immunol. 1983 Jan;130(1):428–434. [PubMed] [Google Scholar]
  5. Galfre G., Howe S. C., Milstein C., Butcher G. W., Howard J. C. Antibodies to major histocompatibility antigens produced by hybrid cell lines. Nature. 1977 Apr 7;266(5602):550–552. doi: 10.1038/266550a0. [DOI] [PubMed] [Google Scholar]
  6. Greene B. M., Taylor H. R., Aikawa M. Cellular killing of microfilariae of Onchocerca volvulus: eosinophil and neutrophil-mediated immune serum-dependent destruction. J Immunol. 1981 Oct;127(4):1611–1618. [PubMed] [Google Scholar]
  7. Haque A., Joseph M., Ouaissi M. A., Capron M., Capron A. IgE antibody-mediated cytotoxicity of rat macrophages against microfilaria of Dipetalonema citeae in vitro. Clin Exp Immunol. 1980 Jun;40(3):487–495. [PMC free article] [PubMed] [Google Scholar]
  8. Haque A., Ouaissi A., Joseph M., Capron M., Capron A. IgE antibody in eosinophil- and macrophage-mediated in vitro killing of Dipetalonema viteae microfilariae. J Immunol. 1981 Aug;127(2):716–725. [PubMed] [Google Scholar]
  9. Johnson P., Mackenzie C. D., Suswillo R. R., Denham D. A. Serum-mediated adherence of feline granulocytes to microfilariae of Brugia pahangi in vitro: variations with parasite maturation. Parasite Immunol. 1981 Spring;3(1):69–80. doi: 10.1111/j.1365-3024.1981.tb00386.x. [DOI] [PubMed] [Google Scholar]
  10. Kazura J. W., Davis R. S. Soluble Brugia malayi microfilarial antigens protect mice against challenge by an antibody-dependent mechanism. J Immunol. 1982 Apr;128(4):1792–1796. [PubMed] [Google Scholar]
  11. Kazura J. W., Grove D. I. Stage-specific antibody-dependent eosinophil-mediated destruction of Trichinella spiralis. Nature. 1978 Aug 10;274(5671):588–589. doi: 10.1038/274588a0. [DOI] [PubMed] [Google Scholar]
  12. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  13. LITTLEFIELD J. W. SELECTION OF HYBRIDS FROM MATINGS OF FIBROBLASTS IN VITRO AND THEIR PRESUMED RECOMBINANTS. Science. 1964 Aug 14;145(3633):709–710. doi: 10.1126/science.145.3633.709. [DOI] [PubMed] [Google Scholar]
  14. Laemmli U. K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970 Aug 15;227(5259):680–685. doi: 10.1038/227680a0. [DOI] [PubMed] [Google Scholar]
  15. Lay W. H., Nussenzweig V. Ca++-dependent binding of antigen-19 S antibody complexes to macrophages. J Immunol. 1969 May;102(5):1172–1178. [PubMed] [Google Scholar]
  16. Lay W. H., Nussenzweig V. Receptors for complement of leukocytes. J Exp Med. 1968 Nov 1;128(5):991–1009. doi: 10.1084/jem.128.5.991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Maizels R. M., Partono F., Oemijati S., Denham D. A., Ogilvie B. M. Cross-reactive surface antigens on three stages of Brugia malayi, B. pahangi and B. timori. Parasitology. 1983 Oct;87(Pt 2):249–263. doi: 10.1017/s0031182000052616. [DOI] [PubMed] [Google Scholar]
  18. McCall J. W., Malone J. B., Hyong-Sun A., Thompson P. E. Mongolian jirds (Meriones unguiculatus) infected with Brugia pahangi by the intraperitoneal route: a rich source of developing larvae, adult filariae, and microfilariae. J Parasitol. 1973 Jun;59(3):436–436. [PubMed] [Google Scholar]
  19. McGreevy P. B., Ratiwayanto S., Tuti S., McGreevy M. M., Dennis D. T. Brugia malayi: relationship between anti-sheath antibodies and amicrofilaremia in natives living in an endemic area of South Kalimantan, Borneo. Am J Trop Med Hyg. 1980 Jul;29(4):553–562. doi: 10.4269/ajtmh.1980.29.553. [DOI] [PubMed] [Google Scholar]
  20. Mehta K., Sindhu R. K., Subrahmanyam D., Hopper K., Nelson D. S. IgE-dependent cellular adhesion and cytotoxicity to Litomosoides carinii microfilariae--nature of effector cells. Clin Exp Immunol. 1982 May;48(2):477–484. [PMC free article] [PubMed] [Google Scholar]
  21. Mitchell G. F., Cruise K. M., Garcia E. G., Anders R. F. Hybridoma-derived antibody with immunodiagnostic potential for schistosomiasis japonica. Proc Natl Acad Sci U S A. 1981 May;78(5):3165–3169. doi: 10.1073/pnas.78.5.3165. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Neuberger M. S., Rajewsky K. Activation of mouse complement by monoclonal mouse antibodies. Eur J Immunol. 1981 Dec;11(12):1012–1016. doi: 10.1002/eji.1830111212. [DOI] [PubMed] [Google Scholar]
  23. Parkhouse R. M., Philipp M., Ogilvie B. M. Characterization of surface antigens of Trichinella spiralis infective larvae. Parasite Immunol. 1981 Winter;3(4):339–352. doi: 10.1111/j.1365-3024.1981.tb00412.x. [DOI] [PubMed] [Google Scholar]
  24. Philipp M., Parkhouse R. M., Ogilvie B. M. Changing proteins on the surface of a parasitic nematode. Nature. 1980 Oct 9;287(5782):538–540. doi: 10.1038/287538a0. [DOI] [PubMed] [Google Scholar]
  25. Piessens W. F., Beldekas M. Diethylcarbamazine enhances antibody-mediated cellular adherence to Brugia malayi microfilariae. Nature. 1979 Dec 20;282(5741):845–847. doi: 10.1038/282845a0. [DOI] [PubMed] [Google Scholar]
  26. Subrahmanyam D., Mehta K., Nelson D. S., Rao Y. V., Rao C. K. Immune reactions in human filariasis. J Clin Microbiol. 1978 Aug;8(2):228–232. doi: 10.1128/jcm.8.2.228-232.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Tanner M., Weiss N. Studies on Dipetalonema vitae (Filarioidea). II. Antibody dependent adhesion of peritoneal exudate cells to microfilariae in vitro. Acta Trop. 1978 Jun;35(2):151–160. [PubMed] [Google Scholar]
  28. Walker W. S. Mediation of macrophage cytolytic and phagocytic activities by antibodies of different classes and class-specific Fc-receptors. J Immunol. 1977 Aug;119(2):367–373. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES