Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 May;55(1):115–124.

The regulation of tissue eosinophilia. III. In vitro production of eosinophil-directed chemotactic inhibitory factor by T lymphocytes of complete Freund's adjuvant-treated guinea-pigs.

K Tashiro, K Sakata, M Hirashima, H Hayashi
PMCID: PMC1453598  PMID: 3873403

Abstract

Guinea-pig spleen cells treated with complete Freund's adjuvant (CFA) produce eosinophil-directed chemotactic inhibitory factors (ECIF). The inhibition is selective for the response of eosinophils to delayed ECF-a, which had been isolated from 24-hr-old inflamed skin lesions induced by DNP-Ascaris extract in sensitized animals and had been confirmed as being a T lymphocyte-derived lymphokine. ECIF activity is absorbed by incubation with eosinophils, but not with macrophages or neutrophils. The cells spontaneously release ECIF; pretreatment with a protein synthesis inhibitor reduces ECIF production, indicating that protein synthesis is essential. The source of ECIF seems to be lymphocytes, probably T lymphocytes. ECIF activity is recovered in two separate fractions: one elutes near bovine serum albumin (MW 70,000) and the other near cytochrome c (MW 12,500). ECIF binds to peanut agglutinin- or Limulus polyphemus agglutinin-coupled agarose beads. Furthermore, ECIF activity is blocked when eosinophils are incubated with D-galactose or sialic acid. These results suggest that ECIF derived from T lymphocytes of CFA-treated animals modulate the delayed ECF-a-mediated tissue eosinophilia, and that terminal galactose and/or sialic acid residues are essential for ECIF activity.

Full text

PDF
115

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DeFranco M. F., Hanson J. M., Morley J., Wolstencroft R. A., Dumonde D. C. Leucocyte chemotactic activity in the parallel bioassay of guinea--pig lymphokines. Immunology. 1977 Feb;32(2):179–190. [PMC free article] [PubMed] [Google Scholar]
  2. Hirashima M., Hayashi H. The mediation of tissue eosinophilia in hypersensitivity reaction. I. Isolation of two different chemotactic factors from DNP-Ascaris extract-induced skin lesion in guinea-pig. Immunology. 1976 Feb;30(2):203–212. [PMC free article] [PubMed] [Google Scholar]
  3. Hirashima M., Honda M., Hayashi H. The mediation of tissue eosinophilia in hypersensitivity reactions. II. Separation of a delayed eosinophil chemotactic factor from macrophage chemotactic factors. Immunology. 1976 Aug;31(2):263–271. [PMC free article] [PubMed] [Google Scholar]
  4. Hirashima M., Tashiro K., Hayashi H. The appearance of eosinophil-directed chemotactic inhibitory factor in the serum of complete Freund's adjuvant-treated guinea pigs. Cell Immunol. 1984 Nov;89(1):113–121. doi: 10.1016/0008-8749(84)90202-8. [DOI] [PubMed] [Google Scholar]
  5. Hirashima M., Tashiro K., Hayashi H. The regulation of tissue eosinophil chemotactic factor and inhibitor in allergic skin lesions of Freund's complete adjuvant-treated guinea-pigs. Immunology. 1984 Mar;51(3):441–450. [PMC free article] [PubMed] [Google Scholar]
  6. Hirashima M., Tashiro K., Sakata K., Yoshimura T., Hayashi H. Difference in requirement of macrophage products for concanavalin A-induced production of eosinophil chemotactic factor and macrophage chemotactic factor from guinea pig lymphocytes in vitro. J Immunol. 1984 Oct;133(4):2012–2017. [PubMed] [Google Scholar]
  7. Hirashima M., Yodoi J., Huff T. F., Ishizaka K. Formation of IgE-binding factors by rat T lymphocytes. III. Mechanisms of selective formation of IgE-suppressive factors by treatment with complete Freund's adjuvant. J Immunol. 1981 Nov;127(5):1810–1816. [PubMed] [Google Scholar]
  8. Hirashima M., Yodoi J., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. IV. Formation of IgE-binding factors in rats treated with complete Freund's adjuvant. J Immunol. 1980 Nov;125(5):2154–2160. [PubMed] [Google Scholar]
  9. Mitchell E. B., Askenase P. W. Suppression of T cell-mediated cutaneous basophil hypersensitivity by serum from guinea pigs immunized with mycobacterial adjuvant. J Exp Med. 1982 Jul 1;156(1):159–172. doi: 10.1084/jem.156.1.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Poste G., Allen H., Matta K. L. Cell surface receptors for lymphokines. II. Studies on the carbohydrate composition of the MIF receptor on macrophages using synthetic saccharides and plant lectins. Cell Immunol. 1979 Apr;44(1):89–98. doi: 10.1016/0008-8749(79)90030-3. [DOI] [PubMed] [Google Scholar]
  11. Poste G., Kirsh R., Fidler I. J. Cell surface receptors for lymphokines. I. The possible role of glycolipids as receptors for macrophage migration inhibitory factor (MIF) and macrophage activation factor (MAF). Cell Immunol. 1979 Apr;44(1):71–88. doi: 10.1016/0008-8749(79)90029-7. [DOI] [PubMed] [Google Scholar]
  12. Remold H. G., David J. R. Further studies on migration inhibitory factor (MIF): evidence for its glycoprotein nature. J Immunol. 1971 Oct;107(4):1090–1098. [PubMed] [Google Scholar]
  13. Remold H. G. Requirement for alpha-L-fucose on the macrophage membrane receptor for MIF. J Exp Med. 1973 Nov 1;138(5):1065–1076. doi: 10.1084/jem.138.5.1065. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Rocklin R. E. Role of monosaccharides in the interaction of two lymphocyte mediators with their target cells. J Immunol. 1976 Mar;116(3):816–820. [PubMed] [Google Scholar]
  15. Ward P. A., Berenberg J. L. Defective regulation of inflammatory mediators in Hodgkin's disease. Supernormal levels of chemotactic-factor inactivator. N Engl J Med. 1974 Jan 10;290(2):76–80. doi: 10.1056/NEJM197401102900203. [DOI] [PubMed] [Google Scholar]
  16. Yodoi J., Hirashima M., Ishizaka K. Regulatory role of IgE-binding factors from rat T lymphocytes. II. Glycoprotein nature and source of IgE-potentiating factor. J Immunol. 1980 Oct;125(4):1436–1441. [PubMed] [Google Scholar]
  17. van den Berg W. B., Haasakker T. C., van Maarsseveen A. C., Scheper R. J. Chronic inflammatory reactions in the guinea-pig peritoneal cavity induced by continuous local PPD stimulation. Immune status dependency of the accumulation of lymphocytes and eosinophils. Immunology. 1980 Aug;40(4):673–681. [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES