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. 1985 Jul;55(3):391–397.

Immunobiology of the oral mucosa in the mouse.

N Deslauriers, S Néron, W Mourad
PMCID: PMC1453632  PMID: 2862103

Abstract

The incidence of immunoglobulin (Ig)-synthesizing cells, Thy 1-positive cells and macrophages in the murine oral mucosa was investigated. Immunofluorescence studies of frozen tissue sections showed that IgA-, IgM- and IgG-containing cells and Thy 1-bearing cells were closely associated with the minor salivary glands. A quantitative analysis was then undertaken using single cell suspensions of the tissue. After mechanical disruption or enzymatic digestion of the mucosa, lymphoid cells were recovered almost exclusively from the mucosa of the posterior soft palate where we observed a dense accumulation of minor salivary glands. Thy 1-bearing cells were found at a higher frequency (25% of recovered cells) than membrane Ig-positive B lymphocytes (6-7%) in these suspensions. Cytoplasmic Ig+ cells accounted for about 6% of recovered cells, whereas plaque-forming cells (Ig-secreting cells) occurred at the same frequency as in the spleen (0.1%). Plasma cells of the IgA and IgM isotypes predominated over IgG-secreting cells (A:M:G ratio = 1:1:0.2); this distribution did not directly correlate with the isotype distribution of salivary Igs (A:M:G ratio = 1:0.003:0.07). In addition, about 10-14% of the cells in our preparations were esterase-positive mononuclear cells. Present data indicate that the murine oral mucosa contains both effector and regulatory cells required for the development and expression of local antibody responses.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abney E. R., Cooper M. D., Kearney J. F., Lawton A. R., Parkhouse R. M. Sequential expression of immunoglobulin on developing mouse B lymphocytes: a systematic survey that suggests a model for the generation of immunoglobulin isotype diversity. J Immunol. 1978 Jun;120(6):2041–2049. [PubMed] [Google Scholar]
  2. Brandtzaeg P. The oral secretory immune system with special emphasis on its relation to dental caries. Proc Finn Dent Soc. 1983;79(2):71–84. [PubMed] [Google Scholar]
  3. Bull D. M., Bookman M. A. Isolation and functional characterization of human intestinal mucosal lymphoid cells. J Clin Invest. 1977 May;59(5):966–974. doi: 10.1172/JCI108719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cebra J. J., Cebra E. R., Clough E. R., Fuhrman J. A., Komisar J. L., Schweitzer P. A., Shahin R. D. IgA commitment: models for B-cell differentiation and possible roles for T-cells in regulating B-cell development. Ann N Y Acad Sci. 1983 Jun 30;409:25–38. doi: 10.1111/j.1749-6632.1983.tb26857.x. [DOI] [PubMed] [Google Scholar]
  5. Crawford J. M., Taubman M. A., Smith D. J. Minor salivary glands as a major source of secretory immunoglobin A in the human oral cavity. Science. 1975 Dec 19;190(4220):1206–1209. doi: 10.1126/science.1198107. [DOI] [PubMed] [Google Scholar]
  6. Eade O. E., Andre-Ukena S. S., Moulton C., MacPherson B., Beeken W. L. Lymphocyte subpopulations of intestinal mucosa in inflammatory bowel disease. Gut. 1980 Aug;21(8):675–682. doi: 10.1136/gut.21.8.675. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ebersole J. L., Taubman M. A., Smith D. J., Crawford J. M. Characterization of immunoglobulin-containing cells in the submandibular gland of the rat after local immunization. Adv Exp Med Biol. 1978;107:155–164. doi: 10.1007/978-1-4684-3369-2_19. [DOI] [PubMed] [Google Scholar]
  8. Emmings F. G., Evans R. T., Genco R. J. Antibody response in the parotid fluid and serum of Irus monkeys (Macaca fascicularis) after local immunization with Streptococcus mutans. Infect Immun. 1975 Aug;12(2):281–292. doi: 10.1128/iai.12.2.281-292.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Frangakis M. V., Koopman W. J., Kiyono H., Michalek S. M., McGhee J. R. An enzymatic method for preparation of dissociated murine Peyer's patch cells enriched for macrophages. J Immunol Methods. 1982;48(1):33–44. doi: 10.1016/0022-1759(82)90207-1. [DOI] [PubMed] [Google Scholar]
  10. Gebhard J. D., Newman J. T., Matthews J. L., Hurt W. C., Stone M. J. Immunopathology of periodontal disease. II. Immunofluorescent studies on the localized immune response in periodontitis and juvenile periodontitis. J Periodontol. 1982 Apr;53(4):239–244. doi: 10.1902/jop.1982.53.4.239. [DOI] [PubMed] [Google Scholar]
  11. Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
  12. Kawanishi H., Saltzman L., Strober W. Mechanisms regulating IgA class-specific immunoglobulin production in murine gut-associated lymphoid tissues. II. Terminal differentiation of postswitch sIgA-bearing Peyer's patch B cells. J Exp Med. 1983 Sep 1;158(3):649–669. doi: 10.1084/jem.158.3.649. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Kawanishi H., Strober W. Regulatory T-cells in murine Peyer's patches directing IgA-specific isotype switching. Ann N Y Acad Sci. 1983 Jun 30;409:243–257. doi: 10.1111/j.1749-6632.1983.tb26874.x. [DOI] [PubMed] [Google Scholar]
  14. Knapp M. J. Oral tonsils: location, distribution, and histology. Oral Surg Oral Med Oral Pathol. 1970 Jan;29(1):155–161. doi: 10.1016/0030-4220(70)90422-6. [DOI] [PubMed] [Google Scholar]
  15. Lebendiger M., Lehner T. Characterization of mononuclear cells in the human oral mucosa. Arch Oral Biol. 1981;26(12):1041–1049. doi: 10.1016/0003-9969(81)90115-1. [DOI] [PubMed] [Google Scholar]
  16. Li C. Y., Lam K. W., Yam L. T. Esterases in human leukocytes. J Histochem Cytochem. 1973 Jan;21(1):1–12. doi: 10.1177/21.1.1. [DOI] [PubMed] [Google Scholar]
  17. Mattes M. J., Sharrow S. O., Herberman R. B., Holden H. T. Identification and separation of Thy-1 positive mouse spleen cells active in natural cytotoxicity and antibody-dependent cell-mediated cytotoxicity. J Immunol. 1979 Dec;123(6):2851–2860. [PubMed] [Google Scholar]
  18. Molinari J. A., Ebersole J. L., Platt D. Investigation of secretory immunoglobulins in saliva from germfree mice. Infect Immun. 1974 Dec;10(6):1207–1212. doi: 10.1128/iai.10.6.1207-1212.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nair P. N., Schroeder H. E. Retrograde access of antigens to the minor salivary glands in the monkey Macaca fascicularis. Arch Oral Biol. 1983;28(2):145–152. doi: 10.1016/0003-9969(83)90121-8. [DOI] [PubMed] [Google Scholar]
  20. Parrott D. M., Tait C., MacKenzie S., Mowat A. M., Davies M. D., Micklem H. S. Analysis of the effector functions of different populations of mucosal lymphocytes. Ann N Y Acad Sci. 1983 Jun 30;409:307–320. doi: 10.1111/j.1749-6632.1983.tb26879.x. [DOI] [PubMed] [Google Scholar]
  21. Schroeder H. E., Dörig-Schwarzenbach A. Structure and composition of the oral mucous membrane on the lips and cheeks of the monkey, Macaca fascicularis. Cell Tissue Res. 1982;224(1):89–104. doi: 10.1007/BF00217269. [DOI] [PubMed] [Google Scholar]
  22. Springer T., Galfré G., Secher D. S., Milstein C. Mac-1: a macrophage differentiation antigen identified by monoclonal antibody. Eur J Immunol. 1979 Apr;9(4):301–306. doi: 10.1002/eji.1830090410. [DOI] [PubMed] [Google Scholar]
  23. Tseng J. A population of resting IgM-IgD double-bearing lymphocytes in Peyer's patches: the major precursor cells for IgA plasma cells in the gut lamina propria. J Immunol. 1984 Jun;132(6):2730–2735. [PubMed] [Google Scholar]

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