Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Jul;55(3):427–436.

A novel lymphokine derived from human IgG Fc receptor-bearing B cells: its suppressive effect on activated T and B lymphocytes including tumour cells in vitro.

T Suzuki, Y H Park, M Miyama-Inaba, T Masuda, H Saji, I Nagata, H Uchino
PMCID: PMC1453638  PMID: 3160654

Abstract

Peripheral blood FcR gamma-bearing human B cells, but neither T cells nor adherent cells, produce an immunoregulatory lymphokine after receiving the stimulation of FcR gamma by immune complexes--antibody-sensitized erythrocytes (EA). This factor suppresses polyclonal immunoglobulin (Ig) production of B cells to pokeweed mitogen (PWM) and Nocardia opaca delipidated cell mitogen (NDCM), indicating that not only B cells, but also T cells are targets for this factor. It also inhibits the proliferation of mitogen-activated mononuclear and T and B tumour cells in vitro. The inhibitory effect on tumour cell growth is cytostatic, but not cytotoxic as in the case of lymphotoxin (LT). All these suppressive effects are observed in a HLA-non-restricted manner. Irradiation (2000 rads) of FcR gamma + B cells does not inhibit the production of this suppressive factor, implying that DNA synthesis is unnecessary. Nonstimulated FcR gamma + B cells retain the precursor activity for Ig-forming cells, since mononuclear cells untreated with EA respond to the mitogens, resulting in Ig production. However, it is worthy to note that they lose the activity when stimulated with immune complexes. Thus, the property obtained from human FcR gamma + B cells is similar to, but distinct from, a murine suppressive B-cell factor (SBF) prepared by the same procedure as for the human factor. Nevertheless, the observation in the present studies on the human analogue to murine SBF suggests that this factor, tentatively termed human SBF, appears to be a novel lymphokine which is different from any other factors, including LT, and that FcR-bearing B cells play an important role in the immunoregulatory mechanism in humans, as in the case of mice.

Full text

PDF
427

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bona C., Broder S., Dimitriu A., Waldmann T. A. Polyclonal activation of human B lymphocytes by Nocardia water soluble mitogen (NWSM). Immunol Rev. 1979;45:69–92. doi: 10.1111/j.1600-065x.1979.tb00273.x. [DOI] [PubMed] [Google Scholar]
  2. Dimitriu A., Fauci A. S. Activation of human B lymphocytes. IX. Modulation of antibody production by products of activated macrophages. J Immunol. 1978 Jun;120(6):1818–1823. [PubMed] [Google Scholar]
  3. Galili U., Schlesinger M. The formation of stable E rosettes after neuraminidase treatment of either human peripheral blood lymphocytes or of sheep red blood cells. J Immunol. 1974 May;112(5):1628–1634. [PubMed] [Google Scholar]
  4. Goodwin J. S., Bankhurst A. D., Messner R. P. Suppression of human T-cell mitogenesis by prostaglandin. Existence of a prostaglandin-producing suppressor cell. J Exp Med. 1977 Dec 1;146(6):1719–1734. doi: 10.1084/jem.146.6.1719. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Granger G. A., Williams T. W. Lymphocyte cytotoxicity in vitro: activation and release of a cytotoxic factor. Nature. 1968 Jun 29;218(5148):1253–1254. doi: 10.1038/2181253a0. [DOI] [PubMed] [Google Scholar]
  6. Gronowicz E., Coutinho A., Melchers F. A plaque assay for all cells secreting Ig of a given type or class. Eur J Immunol. 1976 Aug;6(8):588–590. doi: 10.1002/eji.1830060812. [DOI] [PubMed] [Google Scholar]
  7. Henry C., Jerne N. K. Competition of 19S and 7S antigen receptors in the regulation of the primary immune response. J Exp Med. 1968 Jul 1;128(1):133–152. doi: 10.1084/jem.128.1.133. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Janossy G., Greaves M. Functional analysis of murine and human B lymphocyte subsets. Transplant Rev. 1975;24:177–236. doi: 10.1111/j.1600-065x.1975.tb00169.x. [DOI] [PubMed] [Google Scholar]
  9. Knapp W., Baumgartner G. Monocyte-mediated suppression of human B lymphocyte differentiation in vitro. J Immunol. 1978 Sep;121(3):1177–1183. [PubMed] [Google Scholar]
  10. Laughter A. H., Twomey J. J. Suppression of lymphoproliferation by high concentrations of normal human mononuclear leukocytes. J Immunol. 1977 Jul;119(1):173–179. [PubMed] [Google Scholar]
  11. Masuda T., Miyama M., Kuribayashi K., Yodoi J., Takabayashi A., Kyoizumi S. Immunological properties of Fc receptor on lymphocytes. 5. Suppressive regulation of humoral immune response by Fc receptor bearing B lymphocytes. Cell Immunol. 1978 Sep;39(2):238–249. doi: 10.1016/0008-8749(78)90100-4. [DOI] [PubMed] [Google Scholar]
  12. Miyama-Inaba M., Suzuki T., Paku Y. H., Masuda T. Feedback regulation of immune responses by immune complexes; possible involvement of a suppressive lymphokine by FcR gamma-bearing B cell. J Immunol. 1982 Feb;128(2):882–887. [PubMed] [Google Scholar]
  13. Miyama M., Yamada J., Masuda T. Immunological properties of Fc receptor on lymphocytes. 6. Characterization of suppressive B-cell factor (SBF) released from Fc receptor-bearing B cells. Cell Immunol. 1979 Apr;44(1):51–63. doi: 10.1016/0008-8749(79)90027-3. [DOI] [PubMed] [Google Scholar]
  14. Moretta L., Mingari M. C., Moretta A., Cooper M. D. Human T lymphocyte subpopulations: studies of the mechanism by which T cells bearing Fc receptors for IgG suppress T-dependent B cell differentiation induced by pokeweed mitogen. J Immunol. 1979 Mar;122(3):984–990. [PubMed] [Google Scholar]
  15. Neville M. E., Lischner H. W. Activation of Fc receptor-bearing lymphocytes by immune complexes. I. Stimulation of lymphokine production by nonadherent human peripheral blood lymphocytes. J Immunol. 1982 Mar;128(3):1063–1069. [PubMed] [Google Scholar]
  16. Neville M. E., Lischner H. W. Activation of Fc receptor-bearing lymphocytes by immune complexes. II. Killer lymphocytes mediate Fc ligand-induced lymphokine production. J Exp Med. 1981 Dec 1;154(6):1868–1880. doi: 10.1084/jem.154.6.1868. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Park Y. H., Miyama-Inaba M., Suzuki T., Masuda T., Yoshida Y., Uchino H. Immunological characterization of FcR gamma bearing and nonbearing B cells: functional modulation of immune complexes. Cell Immunol. 1984 Feb;83(2):340–350. doi: 10.1016/0008-8749(84)90313-7. [DOI] [PubMed] [Google Scholar]
  18. Perlmann P., Perlmann H., Larsson A., Wåhlin B. Antibody dependent cytolytic effector lymphocytes (K cells) in human blood. J Reticuloendothel Soc. 1975 Apr;17(4):241–250. [PubMed] [Google Scholar]
  19. Rice L., Laughter A. H., Twomey J. J. Three suppressor systems in human blood that modulate lymphoproliferation. J Immunol. 1979 Mar;122(3):991–996. [PubMed] [Google Scholar]
  20. Suzuki T., Miyama-Inaba M., Masuda T., Uchino H., Ishii K. Monoclonal SBF produced by a hybridoma: in-vitro and in-vivo suppression of B tumour-cell proliferation. Immunology. 1983 Dec;50(4):595–603. [PMC free article] [PubMed] [Google Scholar]
  21. Suzuki T., Miyama-Inaba M., Masuda T., Uchino H., Murakami A., Tanaka H. Suppressive B-cell factor (SBF) produced by FcR-bearing B cells; suppression of B, but not non-B-cell proliferation. Immunology. 1983 Sep;50(1):149–157. [PMC free article] [PubMed] [Google Scholar]
  22. Terasaki P. I., Bernoco D., Park M. S., Ozturk G., Iwaki Y. Microdroplet testing for HLA-A, -B, -C, and -D antigens. The Phillip Levine Award Lecture. Am J Clin Pathol. 1978 Feb;69(2):103–120. doi: 10.1093/ajcp/69.2.103. [DOI] [PubMed] [Google Scholar]
  23. Timonen T., Ortaldo J. R., Herberman R. B. Characteristics of human large granular lymphocytes and relationship to natural killer and K cells. J Exp Med. 1981 Mar 1;153(3):569–582. doi: 10.1084/jem.153.3.569. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Waldmann T. A., Blaese R. M., Broder S., Krakauer R. S. Disorders of suppressor immunoregulatory cells in the pathogenesis of immunodeficiency and autoimmunity. Ann Intern Med. 1978 Feb;88(2):226–238. doi: 10.7326/0003-4819-88-2-226. [DOI] [PubMed] [Google Scholar]
  25. Walker S. M., Lee S. C., Lucas Z. J. Cytotoxic activity of lymphocytes. VI. Heterogeneity of cytotoxins in supernatants of mitogen-activated lymphocytes. J Immunol. 1976 Mar;116(3):807–815. [PubMed] [Google Scholar]
  26. Warrington R. J., Olivier S. L., Sauder P. J., Rutherford W. J. Production of migration inhibition factor (MIF) by purified human T cell subpopulations. Clin Exp Immunol. 1981 May;44(2):324–331. [PMC free article] [PubMed] [Google Scholar]
  27. Warrington R. J., Sauder P. J., Olivier S. L., Rutherford W. J., Wilkins J. A. A human T cell-derived soluble factor able to suppress pokeweed mitogen-induced immunoglobulin production. J Immunol. 1983 Jan;130(1):237–241. [PubMed] [Google Scholar]
  28. Wigzell H. Antibody synthesis at the cellular level. Antibody-induced suppression of 7S antibody synthesis. J Exp Med. 1966 Nov 1;124(5):953–969. doi: 10.1084/jem.124.5.953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Yam L. T., Li C. Y., Crosby W. H. Cytochemical identification of monocytes and granulocytes. Am J Clin Pathol. 1971 Mar;55(3):283–290. doi: 10.1093/ajcp/55.3.283. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES