Abstract
We are attempting to raise antibodies against interleukin-3 receptors by immunizing mice with one cloned line of IL-3 dependent cells. In syngeneic mice immunized with a basophil cell line AC-2, serum contains stimulatory activity preferential for the immunizing cells but largely inactive against an alternative IL-3 dependent line of different phenotype. The serum activity corresponds to a fraction containing a cell agglutinin. Supernatants from hybridomas constructed from immune spleen cells raised by direct spleen immunization also contain binding antibodies, of which a select number show phenotype-restricted proliferative activity. We suggest that such antibodies may be recognizing receptors for IL-3, and that phenotypically different IL-3 dependent cells may express different membrane epitopes concerned with interacting with IL-3.
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Selected References
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- Galfrè G., Milstein C. Preparation of monoclonal antibodies: strategies and procedures. Methods Enzymol. 1981;73(Pt B):3–46. doi: 10.1016/0076-6879(81)73054-4. [DOI] [PubMed] [Google Scholar]
- Garland J. M., Aldridge A., Wagstaffe J., Dexter T. M. Studies on the in vivo production of a lymphokine activity, interleukin 3 (IL-3) elaborated by lymphocytes and a myeloid leukaemic line in vitro and the fate of IL-3 dependent cell lines. Br J Cancer. 1983 Aug;48(2):247–259. doi: 10.1038/bjc.1983.180. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Garland J. M., Dexter T. M. 20 alpha-hydroxysteroid dehydrogenase expression in hemopoietic cell cultures and its relationship to interleukin 3. Eur J Immunol. 1982 Dec;12(12):998–1001. doi: 10.1002/eji.1830121203. [DOI] [PubMed] [Google Scholar]
- Gearing A. J., Thorpe R., Spitz L., Spitz M. Use of 'single shot' intrasplenic immunization for production of monoclonal antibodies specific for human IgM. J Immunol Methods. 1985 Feb 11;76(2):337–343. doi: 10.1016/0022-1759(85)90311-4. [DOI] [PubMed] [Google Scholar]
- Palacios R., Garland J. Distinct mechanisms may account for the growth-promoting activity of interleukin 3 on cells of lymphoid and myeloid origin. Proc Natl Acad Sci U S A. 1984 Feb;81(4):1208–1211. doi: 10.1073/pnas.81.4.1208. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Wauwe J. P., De Mey J. R., Goossens J. G. OKT3: a monoclonal anti-human T lymphocyte antibody with potent mitogenic properties. J Immunol. 1980 Jun;124(6):2708–2713. [PubMed] [Google Scholar]