Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Sep;56(1):93–102.

T-suppressor clones derived from murine AMLR.

M H Bocchieri
PMCID: PMC1453655  PMID: 2931354

Abstract

Panels of cloned T-cell lines were derived from the autologous mixed lymphocyte reactions of NZB and C58 mice. These clones were all Thy 1+. In addition, various clones expressed appropriate Ia, Lyt 1 and/or Lyt 2 antigenic specificities. None of these clones produced the lymphokines IL-2, CSF or AMLR-helper factor. The clones suppressed fresh syngeneic AMLR and MLR responses when added at low cell numbers at the initiation of culture. This suppression was not abrogated by treatment with mitomycin c or reversed by the addition of a source of T-cell growth factor. The mechanism of suppression was not cytotoxicity, as the clones were non-cytotoxic for either syngeneic or allogeneic cells. Many of the clones appeared to require the presence of Lyt 2+ cells in the MLR responding population to suppress, and therefore can be classified as T-suppressor inducers. Two clones did not require the Lyt 2+ subset to suppress the MLR, and are therefore T-suppressor effectors.

Full text

PDF
93

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bocchieri M. H., Smith J. B. Detection of autologous mixed lymphocyte reaction responding cells and their precursor frequency in NZB mice. Cell Immunol. 1982 Dec;74(2):345–357. doi: 10.1016/0008-8749(82)90035-1. [DOI] [PubMed] [Google Scholar]
  2. Bocchieri M. H., Wolos J. A., Smith J. B. Primary and secondary autologous mixed-lymphocyte interactions in mice occur via antigens encoded by genes in the I region of the major histocompatibility complex. Cell Immunol. 1981 Nov 15;65(1):142–151. doi: 10.1016/0008-8749(81)90059-9. [DOI] [PubMed] [Google Scholar]
  3. Burns F. D., Marrack P. C., Kappler J. W., Janeway C. A., Jr Functional heterogeneity among the T-derived lymphocytes of the mouse. IV. Nature of spontaneously induced suppressor cells. J Immunol. 1975 Apr;114(4):1345–1347. [PubMed] [Google Scholar]
  4. Fitzharris P., Knight R. A. Generation of suppressor cells in the autologous mixed lymphocyte reaction. Clin Exp Immunol. 1981 Oct;46(1):185–195. [PMC free article] [PubMed] [Google Scholar]
  5. Glimcher L. H., Longo D. L., Green I., Schwartz R. H. Murine syngeneic mixed lymphocyte response. I. Target antigens are self Ia molecules. J Exp Med. 1981 Nov 1;154(5):1652–1670. doi: 10.1084/jem.154.5.1652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Glimcher L. H., Steinberg A. D., House S. B., Green I. The autologous mixed lymphocyte reaction in strains of mice with autoimmune disease. J Immunol. 1980 Oct;125(4):1832–1838. [PubMed] [Google Scholar]
  7. Hom J. T., Talal N. Decreased syngeneic mixed lymphocyte reaction in autoimmune susceptible mice. Scand J Immunol. 1982 Feb;15(2):195–203. doi: 10.1111/j.1365-3083.1982.tb00638.x. [DOI] [PubMed] [Google Scholar]
  8. Howe M. L., Goldstein A. L., Battisto J. R. Isogeneic lymphocyte interaction: recognition of self antigens by cells of the neonatal thymus. Proc Natl Acad Sci U S A. 1970 Oct;67(2):613–619. doi: 10.1073/pnas.67.2.613. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Johnson E. D., Krco C. J., David C. S. Ia antigens are the stimulating determinants in the syngeneic mixed lymphocyte reaction. Transplantation. 1982 May;33(5):552–555. doi: 10.1097/00007890-198205000-00017. [DOI] [PubMed] [Google Scholar]
  10. Kuntz M. M., Innes J. B., Weksler M. E. Lymphocyte transformation induced by autologous cells. IV. Human T-lymphocyte proliferation induced by autologous or allogeneic non-T lymphocytes. J Exp Med. 1976 May 1;143(5):1042–1054. doi: 10.1084/jem.143.5.1042. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lattime E. C., Gillis S., David C., Stutman O. Interleukin 2, production in the syngeneic mixed lymphocyte reaction. Eur J Immunol. 1981 Jan;11(1):67–69. doi: 10.1002/eji.1830110115. [DOI] [PubMed] [Google Scholar]
  12. Merluzzi V. J., Savage D. M., Mertelsmann R., Welte K. Generation of nonspecific murine cytotoxic T cells in vitro by purified human interleukin 2. Cell Immunol. 1984 Mar;84(1):74–84. doi: 10.1016/0008-8749(84)90078-9. [DOI] [PubMed] [Google Scholar]
  13. Opelz G., Kiuchi M., Takasugi M., Terasaki P. I. Autologous stimulation of human lymphocyte subpopulation. J Exp Med. 1975 Nov 1;142(5):1327–1333. doi: 10.1084/jem.142.5.1327. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Palacios R. HLA-DR antigens render interleukin-2-producer T lymphocytes sensitive to interleukin-1. Scand J Immunol. 1981 Sep;14(3):321–326. doi: 10.1111/j.1365-3083.1981.tb00571.x. [DOI] [PubMed] [Google Scholar]
  15. Palacios R., Möller G. T cell growth factor abrogates concanavalin A-induced suppressor cell function. J Exp Med. 1981 May 1;153(5):1360–1365. doi: 10.1084/jem.153.5.1360. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sakane T., Steinberg A. D., Green I. Failure of autologous mixed lymphocyte reactions between T and non-T cells in patients with systemic lupus erythematosus. Proc Natl Acad Sci U S A. 1978 Jul;75(7):3464–3468. doi: 10.1073/pnas.75.7.3464. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Saxena R. K., Saxena Q. B., Adler W. H. Interleukin-2-induced activation of natural killer activity in spleen cells from old and young mice. Immunology. 1984 Apr;51(4):719–726. [PMC free article] [PubMed] [Google Scholar]
  18. Simon M. M., Moll H., Prester M., Nerz G., Eichmann K. Immunoregulation by mouse T-cell clones. I. Suppression and amplification of cytotoxic responses by cloned H-Y-specific cytolytic T lymphocytes. Cell Immunol. 1984 Jun;86(1):206–221. doi: 10.1016/0008-8749(84)90373-3. [DOI] [PubMed] [Google Scholar]
  19. Smith J. B., DeHoratius R. J. Deficient autologous mixed lymphocyte reactions correlate with disease activity in systemic lupus erythematosus and rheumatoid arthritis. Clin Exp Immunol. 1982 Apr;48(1):155–162. [PMC free article] [PubMed] [Google Scholar]
  20. Smith J. B., Knowlton R. P. Activation of suppressor T cells in human autologous mixed lymphocyte culture. J Immunol. 1979 Jul;123(1):419–422. [PubMed] [Google Scholar]
  21. Smith J. B., Knowlton R. P., Koons L. S. Immunologic studies in chronic lymphocytic leukemia: defective stimulation of T-cell proliferation in autologous mixed lymphocyte culture. J Natl Cancer Inst. 1977 Mar;58(3):579–585. doi: 10.1093/jnci/58.3.579. [DOI] [PubMed] [Google Scholar]
  22. Smith J. B., Pasternak R. D. Syngeneic mixed lymphocyte reaction in mice: strain distribution, kinetics, participating cells, and absence in NZB mice. J Immunol. 1978 Nov;121(5):1889–1892. [PubMed] [Google Scholar]
  23. Smith J. B., Wolos J. A., Bocchieri M. H. Mechanisms of help and suppression in the AMLR: studies in normal and NZB mice. Behring Inst Mitt. 1983 May;(72):37–46. [PubMed] [Google Scholar]
  24. Stout R. D. Inhibition of IL 2 responsiveness of mitogen-stimulated lymphocytes by SMLR-generated plastic adherent suppressor cells. Cell Immunol. 1984 Apr 15;85(1):168–178. doi: 10.1016/0008-8749(84)90287-9. [DOI] [PubMed] [Google Scholar]
  25. Thoman M. L., Weigle W. O. Interleukin 2 induction of antigen-nonspecific suppressor cells. Cell Immunol. 1984 Apr 15;85(1):215–224. doi: 10.1016/0008-8749(84)90291-0. [DOI] [PubMed] [Google Scholar]
  26. Watson J. D. Biology and biochemistry of T cell-derived lymphokines. I. The coordinate synthesis of interleukin 2 and colony-stimulating factors in a murine T cell lymphoma. J Immunol. 1983 Jul;131(1):293–297. [PubMed] [Google Scholar]
  27. Wolos J. A., Smith J. B. Helper cells in the autologous mixed lymphocyte reaction. III. Production of helper factor(s) distinct from interleukin 2. J Exp Med. 1982 Dec 1;156(6):1807–1820. doi: 10.1084/jem.156.6.1807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Yamashita U., Shevach E. M. The syngeneic mixed leukocyte reaction: the genetic requirements for the recognition of self resemble the requirements for the recognition of antigen in association with self. J Immunol. 1980 Apr;124(4):1773–1778. [PubMed] [Google Scholar]
  29. Yu D. T., Chiorazzi N., Kunkel H. G. Helper factors derived from autologous mixed lymphocyte cultures. Cell Immunol. 1980 Mar 15;50(2):305–313. doi: 10.1016/0008-8749(80)90285-3. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES