Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Sep;56(1):73–80.

The expression of Fc and complement receptors in young, adult and aged mice.

V Vĕtvicka, L Fornůsek, J Zídková
PMCID: PMC1453674  PMID: 2931351

Abstract

Age-dependent changes in the expression of Fc receptors (FcR) for different isotypes of immunoglobulins and receptors for C3b, C5b and C3bi fragments of complement on the membranes of peritoneal macrophages were studied with mice of different ages. An age-related increase in expression of Fc receptors for IgM, IgE, IgA, IgG2b and IgG3, and a decrease in the expression of Fc receptors for IgG1 was observed. The expression of FcR on macrophages of donors of different ages corresponded with Fc-receptor mediated phagocytosis. The highest number of C3b-binding macrophages was found in aged mice, in contrast to low numbers of C3bi-binding macrophages at this age. The percentage of C5b-binding macrophages was lowest in adult animals. We also observed effective inhibition of binding of the C3b component of complement by preincubation of macrophages with aggregated IgG and vice versa. These observations suggest that fluctuation in expression of Fc but not C receptors may be important to the generalized changes that occur in macrophage function during development and ageing.

Full text

PDF
73

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aizawa S., Mitsui Y. A new cell surface marker of aging in human diploid fibroblasts. J Cell Physiol. 1979 Aug;100(2):383–388. doi: 10.1002/jcp.1041000219. [DOI] [PubMed] [Google Scholar]
  2. Alper C. A., Abramson N., Johnston R. B., Jr, Jandl J. H., Rosen F. S. Studies in vivo and in vitro on an abnormality in the metabolism of C3 in a patient with increased susceptibility to infection. J Clin Invest. 1970 Nov;49(11):1975–1985. doi: 10.1172/JCI106417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Alper C. A., Colten H. R., Rosen F. S., Rabson A. R., Macnab G. M., Gear J. S. Homozygous deficiency of C3 in a patient with repeated infections. Lancet. 1972 Dec 2;2(7788):1179–1181. doi: 10.1016/s0140-6736(72)92598-6. [DOI] [PubMed] [Google Scholar]
  4. Axén R., Porath J., Ernback S. Chemical coupling of peptides and proteins to polysaccharides by means of cyanogen halides. Nature. 1967 Jun 24;214(5095):1302–1304. doi: 10.1038/2141302a0. [DOI] [PubMed] [Google Scholar]
  5. BOYDEN S. V., SORKIN E. The adsorption of antigen by spleen cells previously treated with antiserum in vitro. Immunology. 1960 Jul;3:272–283. [PMC free article] [PubMed] [Google Scholar]
  6. Boltz-Nitulescu G., Bazin H., Spiegelberg H. L. Specificity of fc receptors for IgG2a, IgG1/IgG2b, and IgE on rat macrophages. J Exp Med. 1981 Aug 1;154(2):374–384. doi: 10.1084/jem.154.2.374. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Callard R. E., Basten A., Blanden R. V. Loss of immune competence with age may be due to a qualitative abnormality in lymphocyte membranes. Nature. 1979 Sep 20;281(5728):218–220. doi: 10.1038/281218a0. [DOI] [PubMed] [Google Scholar]
  8. Cossman J., Richman J. A., Mond J. J. Heterogeneity of complement receptor expression on surface immunoglobulin-bearing cells from neonatal and adult mice. Eur J Immunol. 1982 Jan;12(1):4–8. doi: 10.1002/eji.1830120104. [DOI] [PubMed] [Google Scholar]
  9. Dráber P., Zikán J., Vogtísková M. Establishment and characterization of permanent murine hybridomas secreting monoclonal anti-thy-1 antibodies. J Immunogenet. 1980 Dec;7(6):455–474. doi: 10.1111/j.1744-313x.1980.tb00741.x. [DOI] [PubMed] [Google Scholar]
  10. Gerdes J., Naiem M., Mason D. Y., Stein H. Human complement (C3b) receptors defined by a mouse monoclonal antibody. Immunology. 1982 Apr;45(4):645–653. [PMC free article] [PubMed] [Google Scholar]
  11. HJERTEN S., LEVIN O., TISELIUS A. Protein chromatography on calcium phosphate columns. Arch Biochem Biophys. 1956 Nov;65(1):132–155. doi: 10.1016/0003-9861(56)90183-7. [DOI] [PubMed] [Google Scholar]
  12. Hooijkaas H., Benner R., Pleasants J. R., Wostmann B. S. Isotypes and specificities of immunoglobulins produced by germ-free mice fed chemically defined ultrafiltered "antigen-free" diet. Eur J Immunol. 1984 Dec;14(12):1127–1130. doi: 10.1002/eji.1830141212. [DOI] [PubMed] [Google Scholar]
  13. Humes J. L., Burger S., Galavage M., Kuehl F. A., Jr, Wightman P. D., Dahlgren M. E., Davies P., Bonney R. J. The diminished production of arachidonic acid oxygenation products by elicited mouse peritoneal macrophages: possible mechanisms. J Immunol. 1980 May;124(5):2110–2116. [PubMed] [Google Scholar]
  14. Jessen T. E., Barkholt V., Welinder K. G. A simple alternative pathway for hemolytic assay of human complement component C3 using methylamine-treated plasma. J Immunol Methods. 1983 May 27;60(1-2):89–100. doi: 10.1016/0022-1759(83)90338-1. [DOI] [PubMed] [Google Scholar]
  15. Lu C. Y., Calamai E. G., Unanue E. R. A defect in the antigen-presenting function of macrophages from neonatal mice. Nature. 1979 Nov 15;282(5736):327–329. doi: 10.1038/282327a0. [DOI] [PubMed] [Google Scholar]
  16. Makinodan T., Kay M. M. Age influence on the immune system. Adv Immunol. 1980;29:287–330. doi: 10.1016/s0065-2776(08)60047-4. [DOI] [PubMed] [Google Scholar]
  17. PERKINS E. H., MAKINODAN T. THE SUPPRESSIVE ROLE OF MOUSE PERITONEAL PHAGOCYTES IN AGGLUTININ RESPONSE. J Immunol. 1965 May;94:765–777. [PubMed] [Google Scholar]
  18. Rosenthal A. S., Lipsky P. E., Shevach E. M. Macrophage-lymphocyte interaction and antigen recognition. Fed Proc. 1975 Jul;34(8):1743–1748. [PubMed] [Google Scholar]
  19. Ross G. D. Structure and function of membrane complement receptors. Summary. Fed Proc. 1982 Dec;41(14):3089–3093. [PubMed] [Google Scholar]
  20. Rossmann P., Vetvicka V., Zídková J. C3b receptors on the cell membranes of the stimulated mouse macrophages. An immunohistochemical study. Cell Biol Int Rep. 1983 Dec;7(12):1041–1048. doi: 10.1016/0309-1651(83)90009-7. [DOI] [PubMed] [Google Scholar]
  21. Schneider R. J., Kulczycki A., Jr, Law S. K., Atkinson J. P. Isolation of a biologically active macrophage receptor for the third component of complement. Nature. 1981 Apr 30;290(5809):789–792. doi: 10.1038/290789a0. [DOI] [PubMed] [Google Scholar]
  22. Schreiber R. D., Pangburn M. K., Müller-Eberhard H. J. C3 modified at the thiolester site: acquisition of reactivity with cellular C3b receptors. Biosci Rep. 1981 Nov;1(11):873–880. doi: 10.1007/BF01114821. [DOI] [PubMed] [Google Scholar]
  23. Sterzl J., Silverstein A. M. Developmental aspects of immunity. Adv Immunol. 1967;6:337–459. doi: 10.1016/s0065-2776(08)60525-8. [DOI] [PubMed] [Google Scholar]
  24. Tack B. D., Prahl J. W. Third component of human complement: purification from plasma and physicochemical characterization. Biochemistry. 1976 Oct 5;15(20):4513–4521. doi: 10.1021/bi00665a028. [DOI] [PubMed] [Google Scholar]
  25. Tack B. F., Morris S. C., Prahl J. W. Fifth component of human complement: purification from plasma and polypeptide chain structure. Biochemistry. 1979 Apr 17;18(8):1490–1497. doi: 10.1021/bi00575a016. [DOI] [PubMed] [Google Scholar]
  26. Unanue E. R. The regulatory role of macrophages in antigenic stimulation. Adv Immunol. 1972;15:95–165. doi: 10.1016/s0065-2776(08)60684-7. [DOI] [PubMed] [Google Scholar]
  27. Unkeless J. C. Characterization of a monoclonal antibody directed against mouse macrophage and lymphocyte Fc receptors. J Exp Med. 1979 Sep 19;150(3):580–596. doi: 10.1084/jem.150.3.580. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Unkeless J. C., Fleit H., Mellman I. S. Structural Aspects and Heterogeneity of Immunoglobulin Fc Receptors. Adv Immunol. 1981;31:247–270. doi: 10.1016/s0065-2776(08)60922-0. [DOI] [PubMed] [Google Scholar]
  29. Unkeless J. C., Kaplan G., Plutner H., Cohn Z. A. Fc-receptor variants of a mouse macrophage cell line. Proc Natl Acad Sci U S A. 1979 Mar;76(3):1400–1404. doi: 10.1073/pnas.76.3.1400. [DOI] [PMC free article] [PubMed] [Google Scholar]
  30. Walker W. S., Yen S. E. Complement receptor phenotypes of culture-derived murine macrophages. J Cell Physiol. 1982 Mar;110(3):277–284. doi: 10.1002/jcp.1041100310. [DOI] [PubMed] [Google Scholar]
  31. Woda B. A., Yguerabide J., Feldman J. D. Mobility and density of AgB, "Ia", and Fc receptors on the surface of lymphocytes from young and old rats. J Immunol. 1979 Nov;123(5):2161–2167. [PubMed] [Google Scholar]
  32. Zídková J., Vetvicka V., Rossmann P., Dlabac V., Stránsky J. Characterization of the third component of pig complement and its utilization in a C3b receptor study. Experientia. 1984 Sep 15;40(9):975–977. doi: 10.1007/BF01946467. [DOI] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES