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. 1985 Oct;56(2):307–313.

Decreased membrane potential of T lymphocytes in ageing mice: flow cytometric studies with a carbocyanine dye.

J Witkowski, H S Micklem
PMCID: PMC1453692  PMID: 2997025

Abstract

The membrane potential of lymphocytes from young (1-4-month-old) and old (25-37-month-old) CBA/Ca mice was studied with the aid of the fluorescent dye 3,3'-dihexyloxacarbocyanine iodide (DiOC6(3)). In young mice, most B and T lymphocytes showed a high, and equal, degree of polarization. In old animals most, and in the older individuals almost all, T lymphocytes were found to be depolarized; both Lyt-2+ and Lyt-2- subsets were affected. B cells were largely unaffected. Since changes in transmembrane potential, including temporary hyperpolarization, are known to accompany lymphocyte activation, the depolarized state of T cells in old mice may be related to the decline of T-cell function that occurs during senescence.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ash J. F., Fineman R. M., Kalka T., Morgan M., Wire B. Amplification of sodium- and potassium-activated adenosinetriphosphatase in HeLa cells by ouabain step selection. J Cell Biol. 1984 Sep;99(3):971–983. doi: 10.1083/jcb.99.3.971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chandy K. G., DeCoursey T. E., Cahalan M. D., McLaughlin C., Gupta S. Voltage-gated potassium channels are required for human T lymphocyte activation. J Exp Med. 1984 Aug 1;160(2):369–385. doi: 10.1084/jem.160.2.369. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Felber S. M., Brand M. D. Factors determining the plasma-membrane potential of lymphocytes. Biochem J. 1982 May 15;204(2):577–585. doi: 10.1042/bj2040577. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Gelfand E. W., Cheung R. K., Grinstein S. Role of membrane potential in the regulation of lectin-induced calcium uptake. J Cell Physiol. 1984 Dec;121(3):533–539. doi: 10.1002/jcp.1041210312. [DOI] [PubMed] [Google Scholar]
  5. Kiefer H., Blume A. J., Kaback H. R. Membrane potential changes during mitogenic stimulation of mouse spleen lymphocytes. Proc Natl Acad Sci U S A. 1980 Apr;77(4):2200–2204. doi: 10.1073/pnas.77.4.2200. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kiefer H., Schulze R. The membrane potential of lymphocytes changes only in response to specific stimulation. Biosci Rep. 1982 Aug;2(8):583–588. doi: 10.1007/BF01314219. [DOI] [PubMed] [Google Scholar]
  7. Kyte J. Molecular considerations relevant to the mechanism of active transport. Nature. 1981 Jul 16;292(5820):201–204. doi: 10.1038/292201a0. [DOI] [PubMed] [Google Scholar]
  8. Ledbetter J. A., Herzenberg L. A. Xenogeneic monoclonal antibodies to mouse lymphoid differentiation antigens. Immunol Rev. 1979;47:63–90. doi: 10.1111/j.1600-065x.1979.tb00289.x. [DOI] [PubMed] [Google Scholar]
  9. Makinodan T., James S. J., Inamizu T., Chang M. P. Immunologic basis for susceptibility to infection in the aged. Gerontology. 1984;30(5):279–289. doi: 10.1159/000212647. [DOI] [PubMed] [Google Scholar]
  10. Monroe J. G., Cambier J. C. B cell activation. I. Anti-immunoglobulin-induced receptor cross-linking results in a decrease in the plasma membrane potential of murine B lymphocytes. J Exp Med. 1983 Jun 1;157(6):2073–2086. doi: 10.1084/jem.157.6.2073. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Myśliwska J., Witkowski J., Myśliwski A. Proportion of early E rosettes formed by peripheral blood lymphocytes from young and aged subjects. Gerontology. 1981;27(3):140–145. doi: 10.1159/000212462. [DOI] [PubMed] [Google Scholar]
  12. Ritchie A. W., Gray R. A., Micklem H. S. Right angle light scatter: a necessary parameter in flow cytofluorimetric analysis of human peripheral blood mononuclear cells. J Immunol Methods. 1983 Nov 11;64(1-2):109–117. doi: 10.1016/0022-1759(83)90389-7. [DOI] [PubMed] [Google Scholar]
  13. Rosoff P. M., Cantley L. C. Increasing the intracellular Na+ concentration induces differentiation in a pre-B lymphocyte cell line. Proc Natl Acad Sci U S A. 1983 Dec;80(24):7547–7550. doi: 10.1073/pnas.80.24.7547. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Shapiro H. M. Flow cytometric probes of early events in cell activation. Cytometry. 1981 Mar;1(5):301–312. doi: 10.1002/cyto.990010502. [DOI] [PubMed] [Google Scholar]
  15. Shapiro H. M., Natale P. J., Kamentsky L. A. Estimation of membrane potentials of individual lymphocytes by flow cytometry. Proc Natl Acad Sci U S A. 1979 Nov;76(11):5728–5730. doi: 10.1073/pnas.76.11.5728. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Witkowski J. The influence of the membrane sodium pump activity on the ability of human peripheral blood lymphocytes to form early rosettes with SRBC. Immunology. 1985 Jun;55(2):339–345. [PMC free article] [PubMed] [Google Scholar]

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