Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Oct;56(2):245–251.

Reversal of antibody-dependent cellular cytotoxicity suppression by cycloheximide.

G Serebrinsky, M A Isturiz
PMCID: PMC1453693  PMID: 4054944

Abstract

Antibody-dependent cell-mediated cytotoxicity (ADCC) carried out by fresh and precultured peripheral blood mononuclear cells (PBMC) was compared. The results indicate that the cytotoxic capability was decreased in precultured PBMC, probably due to the effect of suppressor cells. The depletion of adherent cells before preincubation eliminated inhibitory effects, suggesting that the suppressor activity resided in this cell fraction. In addition, supernatants from adherent cell cultures were also able to suppress ADCC of non-adherent PBMC. Normal ADCC values were restored by supplementing the reaction medium with cycloheximide (Cy) 1 X 10(-3) M. The enhancing effect of Cy was exerted upon whole and adherent PBMC, while the opposite effect was observed when ADCC was carried out by non-adherent cells. The fact that Cy increased ADCC activity by precultured PBMC might rule out the possibility that suppression could be ascribed to trivial factors such as cell death, overcrowding or steric hindrance. The results presented in this report support the premise that ADCC is under active immunoregulation.

Full text

PDF
245

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Huerta-Bahena J., Villalobos-Molina R., García-Sáinz J. A. Cycloheximide: an adrenergic agent. Life Sci. 1982 May 17;30(20):1757–1762. doi: 10.1016/0024-3205(82)90310-1. [DOI] [PubMed] [Google Scholar]
  2. Isturiz M. A., de Bracco M. M., Manni J. A. Reaction of human lymphocytes with aggregated IgG columns. Effect on antibody-dependent cytotoxicity and EAC rosette formation. Cell Immunol. 1975 Mar;16(1):82–91. doi: 10.1016/0008-8749(75)90187-2. [DOI] [PubMed] [Google Scholar]
  3. Isturiz M., Cardoni R. L. The role of protein synthesis in antibody-dependent cell-mediated cytotoxicity. Cell Immunol. 1977 Jun 15;31(2):332–339. doi: 10.1016/0008-8749(77)90034-x. [DOI] [PubMed] [Google Scholar]
  4. Lowell G. H., Smith L. F., Artenstein M. S., Nash G. S., MacDermott R. P., Jr Antibody-dependent cell-mediated antibacterial activity of human mononuclear cells. I. K lymphocytes and monocytes are effective against meningococi in cooperation with human imune sera. J Exp Med. 1979 Jul 1;150(1):127–137. doi: 10.1084/jem.150.1.127. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Mahowald M. L., Dalmasso A. P., Messner R. P. Endogenous inhibition of autologous lymphocyte antibody-dependent cellular cytotoxicity. Clin Immunol Immunopathol. 1983 Nov;29(2):211–222. doi: 10.1016/0090-1229(83)90025-9. [DOI] [PubMed] [Google Scholar]
  6. Mkwananzi J. B., Franks D., Baker J. R. Cytotoxicity of antibody-coated trypanosomes by normal human lymphoid cells. Nature. 1976 Feb 5;259(5542):403–404. doi: 10.1038/259403a0. [DOI] [PubMed] [Google Scholar]
  7. Nair M. P., Schwartz S. A. Suppression of natural killer activity and antibody-dependent cellular cytotoxicity by cultured human lymphocytes. J Immunol. 1981 Jun;126(6):2221–2229. [PubMed] [Google Scholar]
  8. Ortiz de Landazuri M., Kedar E., Fahey J. L. Antibody-dependent cellular cytotoxicity to a syngeneic Gross virus-induced lymphoma. J Natl Cancer Inst. 1974 Jan;52(1):147–152. doi: 10.1093/jnci/52.1.147. [DOI] [PubMed] [Google Scholar]
  9. Perlmann P., Holm G. Cytotoxic effects of lymphoid cells in vitro. Adv Immunol. 1969;11:117–193. doi: 10.1016/s0065-2776(08)60479-4. [DOI] [PubMed] [Google Scholar]
  10. Perlmann P., Perlmann H. Contactual lysis of antibody-coated chicken erythrocytes by purified lymphocytes. Cell Immunol. 1970 Sep;1(3):300–315. doi: 10.1016/0008-8749(70)90051-1. [DOI] [PubMed] [Google Scholar]
  11. Perlmann P., Perlmann H., Wigzell H. Lymphocyte mediated cytotoxicity in vitro. Induction and inhibition by humoral antibody and nature of effector cells. Transplant Rev. 1972;13:91–114. doi: 10.1111/j.1600-065x.1972.tb00061.x. [DOI] [PubMed] [Google Scholar]
  12. Pollack S. B. Effector cells for antibody-dependent cell mediated cytotoxicity. I. Increased cytotoxicity after priming with BCG-SS. Cell Immunol. 1977 Mar 15;29(2):373–381. doi: 10.1016/0008-8749(77)90332-x. [DOI] [PubMed] [Google Scholar]
  13. Pollack S. B., Emmons S. I. Inhibition of human killer cells by autologous lymphocytes. J Immunol. 1979 Feb;122(2):718–722. [PubMed] [Google Scholar]
  14. Pollack S. B., Herrick M. V. Inhibition of antibody-dependent cellular cytotoxicity by autologous lymph node cells. J Immunol. 1977 Dec;119(6):2172–2178. [PubMed] [Google Scholar]
  15. Shore S. L., Nahmias A. J., Starr S. E., Wood P. A., McFarlin D. E. Detection of cell-dependent cytotoxic antibody to cells infected with herpes simplex virus. Nature. 1974 Sep 27;251(5473):350–352. doi: 10.1038/251350a0. [DOI] [PubMed] [Google Scholar]
  16. Soper W. D., Bartlett S. P., Winn H. J. Lysis of antibody-coated cells by platelets. J Exp Med. 1982 Oct 1;156(4):1210–1221. doi: 10.1084/jem.156.4.1210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Sármay G., Benczur M., Petrányi G., Klein E., Kahn M., Stanworth D. R., Gergely J. Ligand inhibition studies on the role of Fc receptors in antibody-dependent cell-mediated cytotoxicity. Mol Immunol. 1984 Jan;21(1):43–51. doi: 10.1016/0161-5890(84)90088-9. [DOI] [PubMed] [Google Scholar]
  18. Ting A., Terasaki P. I. Influence of lymphocyte-dependent antibodies on human kidney transplants. Transplantation. 1974 Oct;18(4):371–373. [PubMed] [Google Scholar]
  19. Trinchieri G., Bauman P., De Marchi M., Tökés Z. Antibody-dependent cell-mediated cytotoxicity in humans. I. Characterization of the effector cell. J Immunol. 1975 Jul;115(1):249–255. [PubMed] [Google Scholar]
  20. Wititsuwannakul D., Kim K. H. Mechanism of glycogenolytic action of cycloheximide in rat liver. Biochem Biophys Res Commun. 1978 Feb 28;80(4):1007–1012. doi: 10.1016/0006-291x(78)91345-1. [DOI] [PubMed] [Google Scholar]
  21. Yust I., Wunderlich J. R., Mann D. L., Buell D. N. Cytotoxicity mediated by human lymphocyte-dependent antibody in a rapid assay with adherent target cells. J Immunol. 1973 Jun;110(6):1672–1681. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES