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. 1985 Oct;56(2):301–306.

Surface markers on lymphocytes leaving pig lymph nodes.

R Pabst, R M Binns, S T Licence
PMCID: PMC1453694  PMID: 3876982

Abstract

Mesenteric lymph nodes of normal young pigs were perfused in vitro at physiological temperature. Cell-free perfusion medium was pumped into the artery for more than 2.5 hr, and lymphocytes were continuously released into the venous effluent. Recirculating lymphocytes emigrate from pig lymph nodes by entering the blood vasculature directly and not via efferent lymphatics. The presence of lymphocytes in paracortical venular walls after 2 hr of perfusion with new medium suggests that these are the sites of emigration. The rate of emigration of lymphocytes from mesenteric lymph nodes was estimated to be 6 X 10(7)/hr. Study of the lymphocyte populations emerging from the perfused lymph nodes showed that B lymphocytes and E-rosette forming T lymphocytes, but almost no Null lymphocytes, are involved. While the proportion of B lymphocytes remained constant during the perfusion period, E-rosette forming lymphocytes increased significantly. Lymphocyte subpopulations differ profoundly in their capacity to migrate through lymph nodes.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Binns R. M., Licence S. T., Symons D. B., Gurner B. W., Coombs R. R., Walters D. E. Comparison of the direct antiglobulin rosetting reaction (DARR) and direct immunofluorescence (DIF) for demonstration of sIg-bearing lymphocytes in pigs, sheep and cattle. Immunology. 1979 Mar;36(3):549–555. [PMC free article] [PubMed] [Google Scholar]
  2. Binns R. M. Organisation of the lymphoreticular system and lymphocyte markers in the pig. Vet Immunol Immunopathol. 1982 Jan;3(1-2):95–146. doi: 10.1016/0165-2427(82)90033-2. [DOI] [PubMed] [Google Scholar]
  3. Binns R. M., Pabst R., Licence S. T. Classification of lymphocytes recirculating in the spleen. Immunology. 1981 Oct;44(2):273–279. [PMC free article] [PubMed] [Google Scholar]
  4. Binns R. M., Pabst R., Licence S. T. Lymphocyte emigration from lymph nodes by blood in the pig and efferent lymph in the sheep. Immunology. 1985 Jan;54(1):105–111. [PMC free article] [PubMed] [Google Scholar]
  5. Binns R. M. Sheep erythrocyte rosettes in pigs, sheep, cattle and goats demonstrated in the presence of dextran. J Immunol Methods. 1978;21(3-4):197–210. doi: 10.1016/0022-1759(78)90146-1. [DOI] [PubMed] [Google Scholar]
  6. Fossum S., Smith M. E., Ford W. L. The migration of lymphocytes across specialized vascular endothelium VII. The migration of T and B lymphocytes from the blood of the athymic, nude rat. Scand J Immunol. 1983 Jun;17(6):539–550. doi: 10.1111/j.1365-3083.1983.tb00822.x. [DOI] [PubMed] [Google Scholar]
  7. Hopkins J., McConnell I. Immunological aspects of lymphocyte recirculation. Vet Immunol Immunopathol. 1984 May;6(1-2):3–33. doi: 10.1016/0165-2427(84)90047-3. [DOI] [PubMed] [Google Scholar]
  8. McFarlin D. E., Binns R. M. Lymph node function and lymphocyte circulation in the pig. Adv Exp Med Biol. 1973;29(0):87–93. doi: 10.1007/978-1-4615-9017-0_13. [DOI] [PubMed] [Google Scholar]
  9. Nieuwenhuis P., Ford W. L. Comparative migration of B- and T-Lymphocytes in the rat spleen and lymph nodes. Cell Immunol. 1976 May;23(2):254–267. doi: 10.1016/0008-8749(76)90191-x. [DOI] [PubMed] [Google Scholar]
  10. Pabst R., Binns R. M. In vivo labelling of the spleen and mesenteric lymph nodes with fluorescein isothiocyanate for lymphocyte migration studies. Immunology. 1981 Oct;44(2):321–329. [PMC free article] [PubMed] [Google Scholar]
  11. Pabst R., Geisler R. The route of migration of lymphocytes from blood to spleen and mesenteric lymph nodes in the pig. Cell Tissue Res. 1981;221(2):361–370. doi: 10.1007/BF00216740. [DOI] [PubMed] [Google Scholar]
  12. Pabst R., Trepel F. The predominant role of the spleen in lymphocyte recirculation. I. Homing of lymphocytes to and release from the isolated perfused pig spleen. Cell Tissue Kinet. 1975 Nov;8(6):529–541. [PubMed] [Google Scholar]
  13. Reinecke G., Pabst R. Subsets of blood, spleen and recirculating lymphocytes in man. Clin Exp Immunol. 1983 Sep;53(3):672–678. [PMC free article] [PubMed] [Google Scholar]
  14. Sedgley M., Ford W. L. The migration of lymphocytes across specialized vascular endothelium. I. The entry of lymphocytes into the isolated mesenteric lymph-node of the rat. Cell Tissue Kinet. 1976 May;9(3):231–243. doi: 10.1111/j.1365-2184.1976.tb01271.x. [DOI] [PubMed] [Google Scholar]

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