Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1987 Sep;62(1):17–22.

Immunochemical cross-reactions between pentraxins of different species.

S Maudsley 1, M B Pepys 1
PMCID: PMC1453724  PMID: 3115892

Abstract

Monospecific antisera were raised by immunization with pentraxins, C-reactive protein (CRP) or serum amyloid P component (SAP), that had been isolated from man and a number of different vertebrate species. These antisera were used to test sera or serous fluids from a range of other vertebrate species and also some invertebrates. Cross-reactions between species were comparatively rare but were seen among primates and among ruminants. There was also cross-reactivity between these two groups. Antisera to plaice pentraxins did not react with sera from higher vertebrates but did recognize antigens in sera from other flat fish. No cross-reactivity was observed between CRP and SAP either within or between species despite the known homologies of amino acid sequence and similarities of structure within the family. Several reactions occurred with sera in which either CRP or SAP had previously not been sought or in which only preliminary investigations had been performed. The proteins detected were: CRP in goat, cow, sheep, cat and lemon sole; SAP in monkey and dab. These findings significantly extend the range of species in which pentraxins are known to be present.

Full text

PDF
17

Images in this article

20Figure 2
on p.20
20Figure 3
on p.20

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. ANDERSON H. C., McCARTY M. The occurrence in the rabbit of an acute phase protein analogous to human C reactive protein. J Exp Med. 1951 Jan;93(1):25–36. doi: 10.1084/jem.93.1.25. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Baltz M. L., de Beer F. C., Feinstein A., Munn E. A., Milstein C. P., Fletcher T. C., March J. F., Taylor J., Bruton C., Clamp J. R. Phylogenetic aspects of C-reactive protein and related proteins. Ann N Y Acad Sci. 1982;389:49–75. doi: 10.1111/j.1749-6632.1982.tb22125.x. [DOI] [PubMed] [Google Scholar]
  3. Caspi D., Baltz M. L., Snel F., Gruys E., Niv D., Batt R. M., Munn E. A., Buttress N., Pepys M. B. Isolation and characterization of C-reactive protein from the dog. Immunology. 1984 Oct;53(2):307–313. [PMC free article] [PubMed] [Google Scholar]
  4. De Beer F. C., Pepys M. B. Isolation of human C-reactive protein and serum amyloid P component. J Immunol Methods. 1982;50(1):17–31. doi: 10.1016/0022-1759(82)90300-3. [DOI] [PubMed] [Google Scholar]
  5. Dillman R. C., Coles E. H. A canine serum fraction analogous to human C-reactive protein. Am J Vet Res. 1966 Nov;27(121):1769–1775. [PubMed] [Google Scholar]
  6. Fernández-Morán H., Marchalonis J. J., Edelman G. M. Electron microscopy of a hemagglutinin from Limulus polyphemus. J Mol Biol. 1968 Mar 14;32(2):467–469. doi: 10.1016/0022-2836(68)90023-5. [DOI] [PubMed] [Google Scholar]
  7. Fletcher T. C., White A., Baldo B. A. Isolation of a phosphorylcholine-containing component from the turbot tapeworm, Bothriocephalus scorpii (Müller), and its reaction with C-reactive protein. Parasite Immunol. 1980 Winter;2(4):237–248. doi: 10.1111/j.1365-3024.1980.tb00056.x. [DOI] [PubMed] [Google Scholar]
  8. Floyd-Smith G., Whitehead A. S., Colten H. R., Francke U. The human C-reactive protein gene (CRP) and serum amyloid P component gene (APCS) are located on the proximal long arm of chromosome 1. Immunogenetics. 1986;24(3):171–176. doi: 10.1007/BF00364745. [DOI] [PubMed] [Google Scholar]
  9. GOTSCHLICH E., STETSON C. A., Jr Immunologic cross-reactions among mammalian acute phase proteins. J Exp Med. 1960 Apr 1;111:441–451. doi: 10.1084/jem.111.4.441. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Lei K. J., Liu T., Zon G., Soravia E., Liu T. Y., Goldman N. D. Genomic DNA sequence for human C-reactive protein. J Biol Chem. 1985 Oct 25;260(24):13377–13383. [PubMed] [Google Scholar]
  11. Mantzouranis E. C., Dowton S. B., Whitehead A. S., Edge M. D., Bruns G. A., Colten H. R. Human serum amyloid P component. cDNA isolation, complete sequence of pre-serum amyloid P component, and localization of the gene to chromosome 1. J Biol Chem. 1985 Jun 25;260(12):7752–7756. [PubMed] [Google Scholar]
  12. Maudsley S., Hind C. R., Munn E. A., Buttress N., Pepys M. B. Isolation and characterization of guinea-pig serum amyloid P component. Immunology. 1986 Oct;59(2):317–322. [PMC free article] [PubMed] [Google Scholar]
  13. Maudsley S., Rowe I. F., de Beer F. C., Munn E. A., Herbert J., Feinstein A., Pepys M. B. Identification and isolation of two pentraxins from bovine serum. Clin Exp Immunol. 1987 Mar;67(3):662–673. [PMC free article] [PubMed] [Google Scholar]
  14. Osmand A. P., Friedenson B., Gewurz H., Painter R. H., Hofmann T., Shelton E. Characterization of C-reactive protein and the complement subcomponent C1t as homologous proteins displaying cyclic pentameric symmetry (pentraxins). Proc Natl Acad Sci U S A. 1977 Feb;74(2):739–743. doi: 10.1073/pnas.74.2.739. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Pepys M. B., Baltz M. L. Acute phase proteins with special reference to C-reactive protein and related proteins (pentaxins) and serum amyloid A protein. Adv Immunol. 1983;34:141–212. doi: 10.1016/s0065-2776(08)60379-x. [DOI] [PubMed] [Google Scholar]
  16. Pepys M. B., Dash A. C., Fletcher T. C., Richardson N., Munn E. A., Feinstein A. Analogues in other mammals and in fish of human plasma proteins, C-reactive protein and amyloid P component. Nature. 1978 May 11;273(5658):168–170. doi: 10.1038/273168a0. [DOI] [PubMed] [Google Scholar]
  17. Pepys M. B., De Beer F. C., Milstein C. P., March J. F., Feinstein A., Butress N., Clamp J. R., Taylor J., Bruton C., Fletcher T. C. C-reactive protein and serum amyloid P component in the plaice (Pleuronectes platessa L.), a marine teleost, are homologous with their human counterparts. Biochim Biophys Acta. 1982 May 21;704(1):123–133. doi: 10.1016/0167-4838(82)90139-x. [DOI] [PubMed] [Google Scholar]
  18. Pepys M. B. Isolation of serum amyloid P-component (protein SAP) in the mouse. Immunology. 1979 Jul;37(3):637–641. [PMC free article] [PubMed] [Google Scholar]
  19. Robey F. A., Liu T. Y. Limulin: a C-reactive protein from Limulus polyphemus. J Biol Chem. 1981 Jan 25;256(2):969–975. [PubMed] [Google Scholar]
  20. Robey F. A., Tanaka T., Liu T. Y. Isolation and characterization of two major serum proteins from the dogfish, Mustelus canis, C-reactive protein and amyloid P component. J Biol Chem. 1983 Mar 25;258(6):3889–3894. [PubMed] [Google Scholar]
  21. Rowe I. F., Soutar A. K., Trayner I. M., Baltz M. L., de Beer F. C., Walker L., Bowyer D., Herbert J., Feinstein A., Pepys M. B. Rabbit and rat C-reactive proteins bind apolipoprotein B-containing lipoproteins. J Exp Med. 1984 Feb 1;159(2):604–616. doi: 10.1084/jem.159.2.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. SAKAMOTO Y. [STUDIES ON FREE AMINO ACIDS AND C-REACTIVE PROTEIN IN PERILYMPH OF GUINEA PIGS]. Nihon Jibiinkoka Gakkai Kaiho. 1964 Aug;67:1204–1214. doi: 10.3950/jibiinkoka.67.8_1204. [DOI] [PubMed] [Google Scholar]
  23. USUI D. [STUDIES ON THE INHIBITION OF THE APPEARANCE OF THE C.R.P. AND AMINO-ACIDS IN THE PERILYMPH ON THE PALATAL IRRITATION OF GUINEA PIG]. Nihon Jibiinkoka Gakkai Kaiho. 1964 Aug;67:1215–1226. [PubMed] [Google Scholar]
  24. Wang C. M., Nguyen N. Y., Yonaha K., Robey F., Liu T. Y. Primary structure of rabbit C-reactive protein. J Biol Chem. 1982 Nov 25;257(22):13610–13615. [PubMed] [Google Scholar]
  25. Winkelhake J. L., Chang R. J. Acute phase (C-reactive) protein-like macromolecules from rainbow trout (Salmo gairdneri). Dev Comp Immunol. 1982 Summer;6(3):481–489. doi: 10.1016/s0145-305x(82)80034-7. [DOI] [PubMed] [Google Scholar]
  26. Woo P., Korenberg J. R., Whitehead A. S. Characterization of genomic and complementary DNA sequence of human C-reactive protein, and comparison with the complementary DNA sequence of serum amyloid P component. J Biol Chem. 1985 Oct 25;260(24):13384–13388. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES