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. 1987 Sep;62(1):119–125.

Mitogen-activated Xenopus laevis lymphocytes produce a T-cell growth factor.

D Watkins 1, N Cohen 1
PMCID: PMC1453736  PMID: 2958404

Abstract

Mitogen-free and serum-free supernatants (SNs) from cultures of phytohaemagglutinin (PHA)-stimulated Xenopus splenocytes, co-stimulated thymocytes, induced proliferation of splenic and thymic lymphoblast and supported growth of alloreactive T-cell lines. These SNs had no effect on 'resting' splenocytes, as measured by uptake of tritiated thymidine ([3H]TdR). Growth-promoting activity was also detected in SNs of cultures containing alloreactive T-cell lines and either PHA or irradiated stimulator cells that expressed the original priming alloantigens. Thus, T lymphocytes appear to be involved in producing, as well as responding to, a Xenopus T-cell growth factor (TCGF). TCGF activity could be absorbed from these active SNs with PHA-activated splenic blasts. No functional cross-reactivity among different mammalian interleukin-2 (IL-2) and Xenopus TCGF preparations was detected.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adler W. H., Takiguchi T., Marsh B., Smith R. T. Cellular recognition by mouse lymphocytes in vitro. I. Definition of a new technique and results of stimulation by phytohemagglutinin and specific antigens. J Exp Med. 1970 Jun 1;131(6):1049–1078. doi: 10.1084/jem.131.6.1049. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berzins T., Wikén M., Hellström U., Perlmann P. The use of supernatants from neuraminidase and galactose oxidase (NAGO)-treated lymphocytes as a source of T cell growth factor. J Immunol Methods. 1983 Oct 28;63(3):309–319. doi: 10.1016/s0022-1759(83)80004-0. [DOI] [PubMed] [Google Scholar]
  3. Brown W. C., Grab D. J. Biological and biochemical characterization of bovine interleukin 2. Studies with cloned bovine T cells. J Immunol. 1985 Nov;135(5):3184–3190. [PubMed] [Google Scholar]
  4. Cantrell D. A., Smith K. A. Transient expression of interleukin 2 receptors. Consequences for T cell growth. J Exp Med. 1983 Dec 1;158(6):1895–1911. doi: 10.1084/jem.158.6.1895. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Caspi R. R., Avtalion R. R. Evidence for the existence of an IL-2-like lymphocyte growth promoting factor in a bony fish, Cyprinus carpio. Dev Comp Immunol. 1984 Winter;8(1):51–60. doi: 10.1016/0145-305x(84)90009-0. [DOI] [PubMed] [Google Scholar]
  6. Caspi R. R., Shahrabani R., Kehati-Dan T., Avtalion R. R. Heterogeneity of mitogen-responsive lymphocytes in carp (Cyprinus carpio). Dev Comp Immunol. 1984 Winter;8(1):61–70. doi: 10.1016/0145-305x(84)90010-7. [DOI] [PubMed] [Google Scholar]
  7. Di Sabato G. Purification and initial characterization of rat interleukin 2. Proc Natl Acad Sci U S A. 1982 May;79(9):3020–3023. doi: 10.1073/pnas.79.9.3020. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Ely J. M., Fitch F. W. Alloreactive cloned T cell lines. VII. Comparison of the kinetics of IL 2 release stimulated by alloantigen or Con A. J Immunol. 1983 Sep;131(3):1274–1279. [PubMed] [Google Scholar]
  9. Engers H. D., Collavo D., North M., von Boehmer H., Haas W., Hengartner H., Nabholz M. Characterization of cloned murine cytolytic T cell lines. J Immunol. 1980 Oct;125(4):1481–1486. [PubMed] [Google Scholar]
  10. Gearing A., Rimmer J. J. Amphibian lymphokines: 1. Leucocyte chemotactic factors produced by amphibian spleen cells following antigenic and mitogenic challenge in vitro. Dev Comp Immunol. 1985 Spring;9(2):281–290. doi: 10.1016/0145-305x(85)90119-3. [DOI] [PubMed] [Google Scholar]
  11. Gearing A., Rimmer J. J. Amphibian lymphokines: 2. Migration inhibition factor produced by antigenic and mitogenic stimulation of amphibian leucocytes. Dev Comp Immunol. 1985 Spring;9(2):291–300. doi: 10.1016/0145-305x(85)90120-x. [DOI] [PubMed] [Google Scholar]
  12. Gillis S., Ferm M. M., Ou W., Smith K. A. T cell growth factor: parameters of production and a quantitative microassay for activity. J Immunol. 1978 Jun;120(6):2027–2032. [PubMed] [Google Scholar]
  13. Grondel J. L., Harmsen E. G. Phylogeny of interleukins: growth factors produced by leucocytes of the cyprinid fish, Cyprinus carpio L. Immunology. 1984 Jul;52(3):477–482. [PMC free article] [PubMed] [Google Scholar]
  14. Harwell L., Skidmore B., Marrack P., Kappler J. Concanavalin A-inducible, interleukin-2-producing T cell hybridoma. J Exp Med. 1980 Oct 1;152(4):893–904. doi: 10.1084/jem.152.4.893. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Henderson L. E., Hewetson J. F., Hopkins R. F., 3rd, Sowder R. C., Neubauer R. H., Rabin H. A rapid, large scale purification procedure for gibbon interleukin 2. J Immunol. 1983 Aug;131(2):810–815. [PubMed] [Google Scholar]
  16. Kaye J., Gillis S., Mizel S. B., Shevach E. M., Malek T. R., Dinarello C. A., Lachman L. B., Janeway C. A., Jr Growth of a cloned helper T cell line induced by a monoclonal antibody specific for the antigen receptor: interleukin 1 is required for the expression of receptors for interleukin 2. J Immunol. 1984 Sep;133(3):1339–1345. [PubMed] [Google Scholar]
  17. Mier J. W., Gallo R. C. The purification and properties of human T cell growth factor. J Immunol. 1982 Mar;128(3):1122–1127. [PubMed] [Google Scholar]
  18. Mishell R. I., Dutton R. W. Immunization of dissociated spleen cell cultures from normal mice. J Exp Med. 1967 Sep 1;126(3):423–442. doi: 10.1084/jem.126.3.423. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nagata S. A cell surface marker of thymus-dependent lymphocytes in Xenopus laevis is identifiable by mouse monoclonal antibody. Eur J Immunol. 1985 Aug;15(8):837–841. doi: 10.1002/eji.1830150818. [DOI] [PubMed] [Google Scholar]
  20. Novogrodsky A., Stenzel K. H., Rubin A. L. Stimulation of human peripheral blood lymphocytes by periodate, galactose oxidase, soybean agglutinin, and peanut agglutinin: differential effects of adherent cells. J Immunol. 1977 Mar;118(3):852–857. [PubMed] [Google Scholar]
  21. Novogrodsky A., Suthanthiran M., Saltz B., Newman D., Rubin A. L., Stenzel K. H. Generation of a lymphocyte growth factor by treatment of human cells with neuraminidase and galactose oxidase. J Exp Med. 1980 Mar 1;151(3):755–760. doi: 10.1084/jem.151.3.755. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Paetkau V., Mills G., Gerhart S., Monticone V. Proliferation of murine thymic lymphocytes in vitro is mediated by the concanavalin A-induced release of a lymphokine (costimulator). J Immunol. 1976 Oct;117(4):1320–1324. [PubMed] [Google Scholar]
  23. Robb R. J., Munck A., Smith K. A. T cell growth factor receptors. Quantitation, specificity, and biological relevance. J Exp Med. 1981 Nov 1;154(5):1455–1474. doi: 10.1084/jem.154.5.1455. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Ruben L. N., Clothier R. H., Balls M. Murine and human interleukin 2 can substitute for the thymus in immune responses to TNP-Ficoll in Xenopus laevis, the South African clawed toad. Cell Immunol. 1985 Jun;93(1):229–233. doi: 10.1016/0008-8749(85)90403-4. [DOI] [PubMed] [Google Scholar]
  25. Schauenstein K., Globerson A., Wick G. Avian lymphokines: 1. Thymic cell growth factor in supernatants of mitogen stimulated chicken spleen cells. Dev Comp Immunol. 1982 Summer;6(3):533–540. doi: 10.1016/s0145-305x(82)80039-6. [DOI] [PubMed] [Google Scholar]
  26. Schnetzler M., Oommen A., Nowak J. S., Franklin R. M. Characterization of chicken T cell growth factor. Eur J Immunol. 1983 Jul;13(7):560–566. doi: 10.1002/eji.1830130709. [DOI] [PubMed] [Google Scholar]
  27. Smith K. A., Gillis S., Baker P. E., McKenzie D., Ruscetti F. W. T-cell growth factor-mediated T-cell proliferation. Ann N Y Acad Sci. 1979;332:423–432. doi: 10.1111/j.1749-6632.1979.tb47136.x. [DOI] [PubMed] [Google Scholar]
  28. Smith K. A., Ruscetti F. W. T-cell growth factor and the culture of cloned functional T cells. Adv Immunol. 1981;31:137–175. doi: 10.1016/s0065-2776(08)60920-7. [DOI] [PubMed] [Google Scholar]

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