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. 1985 Nov;56(3):481–487.

Inhibition of lipopolysaccharide mitogenicity with characterized anti-lipid A monoclonal antibodies.

R Girard, R Chaby
PMCID: PMC1453740  PMID: 2867028

Abstract

Antibodies to lipid A were raised in mice immunized with non-hydrolysed Bordetella pertussis microorganisms, coated with lipid A isolated from the same bacteria. Anti-lipid A activity of immune sera was measured by radioimmunoassay. Four hybrid cell lines that secrete antibodies directed against the hydrophobic region of B. pertussis lipopolysaccharide were produced by cell fusion between myeloma cells and spleen cells from immunized C3H/He-PAS mice. Differences were observed in the potency of the isolated monoclonal antibodies to inhibit B cell proliferation induced by lipopolysaccharide (LPS) or by lipid A, suggesting a selective recognition of effector sites present on the hydrophobic region of LPS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Apicella M. A., Bennett K. M., Hermerath C. A., Roberts D. E. Monoclonal antibody analysis of lipopolysaccharide from Neisseria gonorrhoeae and Neisseria meningitidis. Infect Immun. 1981 Dec;34(3):751–756. doi: 10.1128/iai.34.3.751-756.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Banerji B., Alving C. R. Lipid A from endotoxin: antigenic activities of purified fractions in liposomes. J Immunol. 1979 Dec;123(6):2558–2562. [PubMed] [Google Scholar]
  3. Cambiaso C. L., Goffinet A., Vaerman J. P., Heremans J. F. Glutaraldehyde-activated aminohexyl- derivative of Sepharose 4B as a new verstile immunoabsorbent. Immunochemistry. 1975 Apr;12(4):273–278. doi: 10.1016/0019-2791(75)90175-5. [DOI] [PubMed] [Google Scholar]
  4. Chaby R., Girard R., Duflot E. Alternation of low and high affinities of secreted and cell-bound antibodies during the anamnestic response of rabbits to Salmonella senftenberg microorganisms. Scand J Immunol. 1979;9(1):87–98. doi: 10.1111/j.1365-3083.1979.tb02710.x. [DOI] [PubMed] [Google Scholar]
  5. Chaby R., Metezeau P., Girard R. Binding of a rhodamine-labeled lipopolysaccharide to lipopolysaccharide-responder and nonresponder lymphocytes. Cell Immunol. 1984 May;85(2):531–541. doi: 10.1016/0008-8749(84)90265-x. [DOI] [PubMed] [Google Scholar]
  6. Drewry D. T., Lomax J. A., Gray G. W., Wilkinson S. G. Studies of lipid A fractions from the lipopolysaccharides of Pseudomonas aeruginosa and Pseudomonas alcaligenes. Biochem J. 1973 Jul;133(3):563–572. doi: 10.1042/bj1330563. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Ey P. L., Prowse S. J., Jenkin C. R. Isolation of pure IgG1, IgG2a and IgG2b immunoglobulins from mouse serum using protein A-sepharose. Immunochemistry. 1978 Jul;15(7):429–436. doi: 10.1016/0161-5890(78)90070-6. [DOI] [PubMed] [Google Scholar]
  8. Fink P. C., Galanos C. Determination of anti-lipid A and lipid A by enzyme immunoassay. Immunobiology. 1981;158(4):380–390. doi: 10.1016/S0171-2985(81)80008-3. [DOI] [PubMed] [Google Scholar]
  9. Galanos C., Freudenberg M. A., Jay F., Nerkar D., Veleva K., Brade H., Strittmatter W. Immunogenic properties of lipid A. Rev Infect Dis. 1984 Jul-Aug;6(4):546–552. doi: 10.1093/clinids/6.4.546. [DOI] [PubMed] [Google Scholar]
  10. Galanos C., Lüderitz O., Westphal O. Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. Eur J Biochem. 1971 Dec 22;24(1):116–122. doi: 10.1111/j.1432-1033.1971.tb19661.x. [DOI] [PubMed] [Google Scholar]
  11. Gustafsson B., Rosén A., Holme T. Monoclonal antibodies against Vibrio cholerae lipopolysaccharide. Infect Immun. 1982 Nov;38(2):449–454. doi: 10.1128/iai.38.2.449-454.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. HUNTER W. M., GREENWOOD F. C. Preparation of iodine-131 labelled human growth hormone of high specific activity. Nature. 1962 May 5;194:495–496. doi: 10.1038/194495a0. [DOI] [PubMed] [Google Scholar]
  13. Hiernaux J. R., Baker P. J., Delisi C., Rudbach J. A. Modulation of the immune response to lipopolysaccharide. J Immunol. 1982 Mar;128(3):1054–1058. [PubMed] [Google Scholar]
  14. Hiernaux J. R., Schroer K. R., Baker P. J., Rudbach J. A., Bona C. Study of the idiotypy of lipopolysaccharide-specific polyclonal and monoclonal antibodies. Eur J Immunol. 1982 Oct;12(10):797–803. doi: 10.1002/eji.1830121002. [DOI] [PubMed] [Google Scholar]
  15. Kannagi R., Nudelman E., Levery S. B., Hakomori S. A series of human erythrocyte glycosphingolipids reacting to the monoclonal antibody directed to a developmentally regulated antigen SSEA-1. J Biol Chem. 1982 Dec 25;257(24):14865–14874. [PubMed] [Google Scholar]
  16. Kirkland T. N., Ziegler E. J. An immunoprotective monoclonal antibody to lipopolysaccharide. J Immunol. 1984 May;132(5):2590–2592. [PubMed] [Google Scholar]
  17. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  18. Le Dur A., Caroff M., Chaby R., Szabó L. A novel type of endotoxin structure present in Bordetella pertussis. Isolation of two different polysaccharides bound to lipid A. Eur J Biochem. 1978 Mar 15;84(2):579–589. doi: 10.1111/j.1432-1033.1978.tb12201.x. [DOI] [PubMed] [Google Scholar]
  19. Lugowski C., Romanowska E. Biological properties of lipid A from Shigella sonnei. Eur J Biochem. 1974 Oct 1;48(1):81–87. doi: 10.1111/j.1432-1033.1974.tb03745.x. [DOI] [PubMed] [Google Scholar]
  20. Mattsby-Baltzer I., Claësson I., Hanson L. A., Jodal U., Kaijser B., Lindberg U., Peterson H. Antibodies to lipid A during urinary tract infection. J Infect Dis. 1981 Oct;144(4):319–328. doi: 10.1093/infdis/144.4.319. [DOI] [PubMed] [Google Scholar]
  21. McCabe W. R., Bruins S. C., Craven D. E., Johns M. Cross-reactive antigens: their potential for immunization-induced immunity to Gram-negative bacteria. J Infect Dis. 1977 Aug;136 (Suppl):S161–S166. doi: 10.1093/infdis/136.supplement.s161. [DOI] [PubMed] [Google Scholar]
  22. Mullan N. A., Newsome P. M., Cunnington P. G., Palmer G. H., Wilson M. E. Protection against gram-negative infections with antiserum to lipid A from Salmonella minnesota R595. Infect Immun. 1974 Dec;10(6):1195–1201. doi: 10.1128/iai.10.6.1195-1201.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Mutharia L. M., Crockford G., Bogard W. C., Jr, Hancock R. E. Monoclonal antibodies specific for Escherichia coli J5 lipopolysaccharide: cross-reaction with other gram-negative bacterial species. Infect Immun. 1984 Sep;45(3):631–636. doi: 10.1128/iai.45.3.631-636.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Nowinski R. C., Lostrom M. E., Tam M. R., Stone M. R., Burnette W. N. The isolation of hybrid cell lines producing monoclonal antibodies against the p15(E) protein of ecotropic murine leukemia viruses. Virology. 1979 Feb;93(1):111–126. doi: 10.1016/0042-6822(79)90280-0. [DOI] [PubMed] [Google Scholar]
  25. Rietschel E. T., Galanos C. Lipid A antiserum-mediated protection against lipopolysaccharide- and lipid A-induced fever and skin necrosis. Infect Immun. 1977 Jan;15(1):34–49. doi: 10.1128/iai.15.1.34-49.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Rioux-Darrieulat F., Parant M., Chedid L. Prevention of endotoxin-induced abortion by treatment of mice with antisera. J Infect Dis. 1978 Jan;137(1):7–13. doi: 10.1093/infdis/137.1.7. [DOI] [PubMed] [Google Scholar]
  27. Shulman M., Wilde C. D., Köhler G. A better cell line for making hybridomas secreting specific antibodies. Nature. 1978 Nov 16;276(5685):269–270. doi: 10.1038/276269a0. [DOI] [PubMed] [Google Scholar]
  28. Simon G., Reindke B., Marget W. Lipoid-A-Antikörpertiter bei Pyelonephritis und anderen infektionen mit gram-negativen Bakterien. Infection. 1974;2(4):178–184. doi: 10.1007/BF01641457. [DOI] [PubMed] [Google Scholar]

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