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. 1985 Aug;55(4):571–577.

Immunoglobulin D in rat serum, saliva and milk.

M G Steele, G A Leslie
PMCID: PMC1453765  PMID: 4018841

Abstract

Previously, this laboratory has found very high concentrations of IgD in normal rat milk. Using ELISA methods, the relationship between milk, serum and saliva IgD in lactating and suckling rats was examined. Milk IgD appears to be synthesized in the mammary tissue rather than taken up from the blood because (i) serum IgD remains low and is not significantly different from that of non-lactating females, and (ii) serum IgD during lactation is poorly correlated with milk IgD. Serum IgD in suckling rats declines in the first 7 days following birth and remains relatively low during the remainder of lactation (2-4 micrograms/ml). The surprisingly high serum IgD observed at birth (9.3 +/- 3.2 micrograms/ml) is present before suckling begins and is not affected by the onset of suckling. Transient elevations of serum IgD begin to occur following weaning. Rats weaned 10 days earlier than normal (Day 20 vs Day 30) had significantly higher serum IgD on Days 36, 47 and 60. Among 43 adult rats, serum IgD was 5.4 +/- 3.6 micrograms/ml and saliva IgD 2.0 +/- 1.7 micrograms/ml. Serum IgD correlates poorly with saliva IgD. The thymus is not required for IgD synthesis since no significant difference in serum IgD was found between nude rats and their euthymic littermates.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abney E. R., Parkhouse R. M. Candidate for immunoglobulin D present on murine B lymphocytes. Nature. 1974 Dec 13;252(5484):600–602. doi: 10.1038/252600a0. [DOI] [PubMed] [Google Scholar]
  2. Bahna S. L., Keller M. A., Heiner D. C. IgE and IgD in human colostrum and plasma. Pediatr Res. 1982 Aug;16(8):604–607. doi: 10.1203/00006450-198208000-00004. [DOI] [PubMed] [Google Scholar]
  3. Bazin H., Beckers A., Urbain-Vansanten G., Pauwels R., Bruyns C., Tilkin A. F., Platteau B., Urbain J. Transplantable IgD immunoglobulin-secreting tumors in rat. J Immunol. 1978 Nov;121(5):2077–2082. [PubMed] [Google Scholar]
  4. Bazin H., Platteau B., Pauwels R., Capron A. Immunoglobulin production in nude rats with special attention to the IgE isotype. Ann Immunol (Paris) 1980 Jan-Feb;131C(1):31–37. [PubMed] [Google Scholar]
  5. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1006/abio.1976.9999. [DOI] [PubMed] [Google Scholar]
  6. Brandtzaeg P. The secretory immune system of lactating human mammary glands compared with other exocrine organs. Ann N Y Acad Sci. 1983 Jun 30;409:353–382. doi: 10.1111/j.1749-6632.1983.tb26883.x. [DOI] [PubMed] [Google Scholar]
  7. Butler J. E. Bovine immunoglobulins: an augmented review. Vet Immunol Immunopathol. 1983 Mar;4(1-2):43–152. doi: 10.1016/0165-2427(83)90056-9. [DOI] [PubMed] [Google Scholar]
  8. Cuchens M. A., Martin L. N., Leslie G. A. The effects of anti-IgD on serum immunoglobulins, antibody production, and immunoglobulin-bearing cells in adult rats. J Immunol. 1978 Dec;121(6):2257–2262. [PubMed] [Google Scholar]
  9. DIXON F. J., WEIGLE W. O., VAZQUEZ J. J. Metabolism and mammary secretion of serum proteins in the cow. Lab Invest. 1961 Mar-Apr;10:216–237. [PubMed] [Google Scholar]
  10. Festing M. F., May D., Connors T. A., Lovell D., Sparrow S. An athymic nude mutation in the rat. Nature. 1978 Jul 27;274(5669):365–366. doi: 10.1038/274365a0. [DOI] [PubMed] [Google Scholar]
  11. Finkelman F. D., Woods V. L., Berning A., Scher I. Demonstration of mouse serum IgD. J Immunol. 1979 Sep;123(3):1253–1259. [PubMed] [Google Scholar]
  12. Golding H., Cuchens M. A., Leslie G. A., Rittenberg M. B. Cross-reactivity of rat, mouse, and human IgD. J Immunol. 1979 Dec;123(6):2751–2755. [PubMed] [Google Scholar]
  13. Halsey J. F., Mitchell C., Meyer R., Cebra J. J. Metabolism of immunoglobulin A in lactating mice: origins of immunoglobulin A in milk. Eur J Immunol. 1982 Feb;12(2):107–112. doi: 10.1002/eji.1830120203. [DOI] [PubMed] [Google Scholar]
  14. Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
  15. Leslie G. A., Armen R. C. Structure and biological functions of human IgD. 3. Phylogenetic studies of IgD. Int Arch Allergy Appl Immunol. 1974;46(2):191–197. doi: 10.1159/000231123. [DOI] [PubMed] [Google Scholar]
  16. Leslie G. A., Martin L. N. Structure and function of serum and membrane immunoglobulin D (IgD). Contemp Top Mol Immunol. 1978;7:1–49. doi: 10.1007/978-1-4757-0779-3_1. [DOI] [PubMed] [Google Scholar]
  17. Martin L. N., Leslie G. A., Hindes R. Lymphocyte surface IgD and IgM in non-human primates. Int Arch Allergy Appl Immunol. 1976;51(3):320–329. doi: 10.1159/000231606. [DOI] [PubMed] [Google Scholar]
  18. Olson J. C., Leslie G. A. IgD: a component of the secretory immune system? Ann N Y Acad Sci. 1982;399:97–104. doi: 10.1111/j.1749-6632.1982.tb25665.x. [DOI] [PubMed] [Google Scholar]
  19. Pauwels R., Bazin H., Platteau B., Van Der Straeten M. The influence of different adjuvants on the production of IgD and IgE antibodies. Ann Immunol (Paris) 1979 Jan-Feb;130C(1):49–58. [PubMed] [Google Scholar]
  20. ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. I. A UNIQUE MYELOMA PROTEIN. J Exp Med. 1965 Jan 1;121:171–184. doi: 10.1084/jem.121.1.171. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. ROWE D. S., FAHEY J. L. A NEW CLASS OF HUMAN IMMUNOGLOBULINS. II. NORMAL SERUM IGD. J Exp Med. 1965 Jan 1;121:185–199. doi: 10.1084/jem.121.1.185. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Rowe D. S., Hug K., Forni L., Pernis B. Immunoglobulin D as a lymphocyte receptor. J Exp Med. 1973 Oct 1;138(4):965–972. doi: 10.1084/jem.138.4.965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Ruddick J. H., Leslie G. A. Structure and biologic functions of human IgD. XI. Identification and ontogeny of a rat lymphocyte immunoglobulin having antigenic cross-reactivity with human IgD. J Immunol. 1977 Mar;118(3):1025–1031. [PubMed] [Google Scholar]
  24. Sire J., Collé A., Bourgois A. Identification of an IgD-like surface immunoglobulin on rabbit lymphocytes. Eur J Immunol. 1979 Jan;9(1):13–16. doi: 10.1002/eji.1830090104. [DOI] [PubMed] [Google Scholar]
  25. Stankus R. P., Leslie G. A. Nonprecipitating and electrophoretically restricted antibodies to carbohydrate of group A Streptococcus in the rat. J Infect Dis. 1974 Aug;130(2):169–173. doi: 10.1093/infdis/130.2.169. [DOI] [PubMed] [Google Scholar]
  26. Zikán J., Síma P., Tucková L. Cross-reactivity of human and a putative pig IgD. Pig IgD-like molecules as serum and lymphocyte components. Folia Microbiol (Praha) 1983;28(6):474–483. doi: 10.1007/BF02879686. [DOI] [PubMed] [Google Scholar]

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