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. 1985 Aug;55(4):639–645.

Erythrocyte lysis by PMA-triggered neutrophil polymorphonuclears: evidence for an hypochlorous acid-dependent process.

F Dallegri, A Ballestrero, G Frumento, F Patrone
PMCID: PMC1453787  PMID: 2991128

Abstract

Human red blood cells (HRBC) were efficiently lysed when incubated with neutrophil polymorphonuclears (PMN) in the presence of phorbol-myristate-acetate (PMA), as detected by a 4-hr 51Cr release assay. The lysis was virtually absent in the presence of catalase, azide or cyanide and in the absence of chloride ions. These findings indicate the involvement of the myeloperoxidase (MPO)-hydrogen peroxide (H2O2)-chloride (Cl-) system in the cytolytic process. As the MPO-H2O2-Cl- system is capable of generating the powerful oxidant hypochlorous acid (HOCl), cytotoxicity assays were performed in the presence of taurine, glycine, serine and valine to scavenge this potentially lytic agent. Each of these compounds efficiently inhibited the HRBC lysis by PMA-triggered PMN, as well as the lysis caused by HOCl in a cell-free system. Thus, the results suggest that HOCl, or an agent with similar reactivity, plays a key role in the PMA-dependent PMN-mediated cytotoxicity against HRBC targets.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Albrich J. M., McCarthy C. A., Hurst J. K. Biological reactivity of hypochlorous acid: implications for microbicidal mechanisms of leukocyte myeloperoxidase. Proc Natl Acad Sci U S A. 1981 Jan;78(1):210–214. doi: 10.1073/pnas.78.1.210. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Bradley P. P., Christensen R. D., Rothstein G. Cellular and extracellular myeloperoxidase in pyogenic inflammation. Blood. 1982 Sep;60(3):618–622. [PubMed] [Google Scholar]
  3. Clark R. A., Szot S. The myeloperoxidase-hydrogen peroxide-halide system as effector of neutrophil-mediated tumor cell cytotoxicity. J Immunol. 1981 Apr;126(4):1295–1301. [PubMed] [Google Scholar]
  4. Dallegri F., Patrone F., Bonvini E., Gahrton G., Holm G., Sacchetti C. Ox erythrocyte cytotoxicity by phorbol myristate acetate-activated human neutrophils. Scand J Immunol. 1983 Feb;17(2):109–114. doi: 10.1111/j.1365-3083.1983.tb00772.x. [DOI] [PubMed] [Google Scholar]
  5. Dallegri F., Patrone F., Frumento G., Ballestrero A., Sacchetti C. Neutrophil-mediated cellular cytotoxicity triggered by immobilized aggregated IgG: an in vitro model of cell injury during immune complex diseases. J Clin Immunol. 1984 Nov;4(6):439–444. doi: 10.1007/BF00916573. [DOI] [PubMed] [Google Scholar]
  6. Grisham M. B., Jefferson M. M., Thomas E. L. Role of monochloramine in the oxidation of erythrocyte hemoglobin by stimulated neutrophils. J Biol Chem. 1984 Jun 10;259(11):6757–6765. [PubMed] [Google Scholar]
  7. Klebanoff S. J., Clark R. A. Hemolysis and iodination of erythrocyte components by a myeloperoxidase-mediated system. Blood. 1975 May;45(5):699–707. [PubMed] [Google Scholar]
  8. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  9. Nathan C., Cohn Z. Role of oxygen-dependent mechanisms in antibody-induced lysis of tumor cells by activated macrophages. J Exp Med. 1980 Jul 1;152(1):198–208. doi: 10.1084/jem.152.1.198. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Nauseef W. M., Metcalf J. A., Root R. K. Role of myeloperoxidase in the respiratory burst of human neutrophils. Blood. 1983 Mar;61(3):483–492. [PubMed] [Google Scholar]
  11. Ruch W., Cooper P. H., Baggiolini M. Assay of H2O2 production by macrophages and neutrophils with homovanillic acid and horse-radish peroxidase. J Immunol Methods. 1983 Oct 28;63(3):347–357. doi: 10.1016/s0022-1759(83)80008-8. [DOI] [PubMed] [Google Scholar]
  12. Weiss S. J., Klein R., Slivka A., Wei M. Chlorination of taurine by human neutrophils. Evidence for hypochlorous acid generation. J Clin Invest. 1982 Sep;70(3):598–607. doi: 10.1172/JCI110652. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Weiss S. J., LoBuglio A. F. An oxygen-dependent mechanism of neutrophil-mediated cytotoxicity. Blood. 1980 Jun;55(6):1020–1024. [PubMed] [Google Scholar]
  14. Weiss S. J., Slivka A. Monocyte and granulocyte-mediated tumor cell destruction. A role for the hydrogen peroxide-myeloperoxidase-chloride system. J Clin Invest. 1982 Feb;69(2):255–262. doi: 10.1172/JCI110447. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Weiss S. J. The role of superoxide in the destruction of erythrocyte targets by human neutrophils. J Biol Chem. 1980 Oct 25;255(20):9912–9917. [PubMed] [Google Scholar]

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