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. 1985 Dec;56(4):673–681.

Mononuclear phagocytes of normal and rheumatoid synovial membrane identified by monoclonal antibodies.

N Hogg, D G Palmer, P A Revell
PMCID: PMC1453794  PMID: 2416682

Abstract

The presence of cells bearing epitopes of the mononuclear phagocyte series was studied immunohistochemically in synovium removed from joints involved by trauma (T), osteoarthritis (OA) and rheumatoid arthritis (RA). Mononuclear phagocytes were the most consistent feature of the inflamed rheumatoid synovium. They shared at least five epitopes expressed by mononuclear phagocytes in other tissues. In OA/T samples, cells bearing markers of the less mature monocyte were present at the surface of the synovial membrane, namely the intimal layer, while those bearing macrophage epitopes were apparent throughout the intimal layer and subintimally. This suggested that maturation of the monocyte population takes place after the monocytes have entered the synovial tissues, settled at the surface, then moved downward into the subintimal layer. The synovial monocytes accounted for all the HLA-D region positive cells in the lining layer.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anderson D. C., Schmalstieg F. C., Arnaout M. A., Kohl S., Tosi M. F., Dana N., Buffone G. J., Hughes B. J., Brinkley B. R., Dickey W. D. Abnormalities of polymorphonuclear leukocyte function associated with a heritable deficiency of high molecular weight surface glycoproteins (GP138): common relationship to diminished cell adherence. J Clin Invest. 1984 Aug;74(2):536–551. doi: 10.1172/JCI111451. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Brodsky F. M., Parham P., Barnstable C. J., Crumpton M. J., Bodmer W. F. Monoclonal antibodies for analysis of the HLA system. Immunol Rev. 1979;47:3–61. doi: 10.1111/j.1600-065x.1979.tb00288.x. [DOI] [PubMed] [Google Scholar]
  3. Burmester G. R., Dimitriu-Bona A., Waters S. J., Winchester R. J. Identification of three major synovial lining cell populations by monoclonal antibodies directed to Ia antigens and antigens associated with monocytes/macrophages and fibroblasts. Scand J Immunol. 1983 Jan;17(1):69–82. doi: 10.1111/j.1365-3083.1983.tb00767.x. [DOI] [PubMed] [Google Scholar]
  4. Burmester G. R., Locher P., Koch B., Winchester R. J., Dimitriu-Bona A., Kalden J. R., Mohr W. The tissue architecture of synovial membranes in inflammatory and non-inflammatory joint diseases. I. The localization of the major synovial cell populations as detected by monoclonal reagents directed towards Ia and monocyte-macrophage antigens. Rheumatol Int. 1983;3(4):173–181. doi: 10.1007/BF00541597. [DOI] [PubMed] [Google Scholar]
  5. Dreher R. Origin of synovial type A cells during inflammation. An experimental approach. Immunobiology. 1982 Apr;161(3-4):232–245. doi: 10.1016/S0171-2985(82)80079-X. [DOI] [PubMed] [Google Scholar]
  6. Duke O., Panayi G. S., Janossy G., Poulter L. W. An immunohistological analysis of lymphocyte subpopulations and their microenvironment in the synovial membranes of patients with rheumatoid arthritis using monoclonal antibodies. Clin Exp Immunol. 1982 Jul;49(1):22–30. [PMC free article] [PubMed] [Google Scholar]
  7. Edwards J. C., Sedgwick A. D., Willoughby D. A. Membrane properties and esterase activity of synovial lining cells: further evidence for a mononuclear phagocyte subpopulation. Ann Rheum Dis. 1982 Jun;41(3):282–286. doi: 10.1136/ard.41.3.282. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Førre O., Thoen J., Lea T., Dobloug J. H., Mellbye O. J., Natvig J. B., Pahle J., Solheim B. G. In situ characterization of mononuclear cells in rheumatoid tissues, using monoclonal antibodies. No reduction of T8-positive cells or augmentation in T4-positive cells. Scand J Immunol. 1982 Oct;16(4):315–319. doi: 10.1111/j.1365-3083.1982.tb00729.x. [DOI] [PubMed] [Google Scholar]
  9. Hogg N., MacDonald S., Slusarenko M., Beverley P. C. Monoclonal antibodies specific for human monocytes, granulocytes and endothelium. Immunology. 1984 Dec;53(4):753–767. [PMC free article] [PubMed] [Google Scholar]
  10. Hogg N., Selvendran Y. An anti-human monocyte/macrophage monoclonal antibody, reacting most strongly with macrophages in lymphoid tissue. Cell Immunol. 1985 May;92(2):247–253. doi: 10.1016/0008-8749(85)90006-1. [DOI] [PubMed] [Google Scholar]
  11. Klareskog L., Forsum U., Wigren A., Wigzell H. Relationship between HLA-DR-expressing cells and T lymphocytes of different subsets in rheumatoid synovial tissue. Scand J Immunol. 1981 May;15(5):501–507. doi: 10.1111/j.1365-3083.1982.tb00676.x. [DOI] [PubMed] [Google Scholar]
  12. Palmer D. G., Selvendran Y., Allen C., Revell P. A., Hogg N. Features of synovial membrane identified with monoclonal antibodies. Clin Exp Immunol. 1985 Mar;59(3):529–538. [PMC free article] [PubMed] [Google Scholar]
  13. Poulter L. W., Duke O., Hobbs S., Janossy G., Panayi G., Seymour G. The involvement of interdigitating (antigen-presenting) cells in the pathogenesis of rheumatoid arthritis. Clin Exp Immunol. 1983 Feb;51(2):247–254. [PMC free article] [PubMed] [Google Scholar]
  14. Roy S., Ghadially F. N., Crane W. A. Synovial membrane in traumatic effusion. Ultrastructure and autoradiography with tritiated leucine. Ann Rheum Dis. 1966 May;25(3):259–271. doi: 10.1136/ard.25.3.259. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Shiozawa S., Shiozawa K., Fujita T. Presence of HLA-DR antigen on synovial type A and B cells: an immunoelectron microscopic study in rheumatoid arthritis, osteoarthritis and normal traumatic joints. Immunology. 1983 Dec;50(4):587–594. [PMC free article] [PubMed] [Google Scholar]

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