Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1985 Dec;56(4):615–623.

Leukaemia x fibroblast hybrid cells augment the antibody response to sheep red blood cells in inbred mice.

E P Cohen, K Hagen
PMCID: PMC1453797  PMID: 3908292

Abstract

ASL-1 x LM(TK-) hybrid cells, an established murine leukaemia x fibroblast hybrid cell line, augment the antibody response to sheep red blood cells in inbred mice, as determined by the plaque assay method. The intraperitoneal injection of viable hybrid cells or of growth medium conditioned by the cells leads to an increase both in the total number as well as the proportion of cells forming antibodies to sheep red blood cells. CSF-1, (M-CSF), is detected by radioimmunoassay in the medium conditioned by the hybrid and LM(TK-) cells, but not ASL-1 parental cells. Prior treatment of the conditioned medium with CSF-1 antiserum reduces its capacity to augment the antibody response, and its proliferative stimulus on cells from the marrow indicating that CSF-1 may be at least partly responsible for the adjuvant effect observed. The intraperitoneal implantation of diffusion chambers containing viable CSF-1 producing hybrid cells, like the cells themselves, also leads to an increase in the number of spleen cells forming antibodies to sheep red blood cells.

Full text

PDF
615

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. DeFreitas E. C., Chesnut R. W., Grey H. M., Chiller J. M. Macrophage-dependent activation of antigen-specific T cells requires antigen and a soluble monokine. J Immunol. 1983 Jul;131(1):23–29. [PubMed] [Google Scholar]
  2. Farrar J. J., Howard M., Fuller-Farrar J., Paul W. E. Biochemical and physicochemical characterization of mouse B cell growth factor: a lymphokine distinct from interleukin 2. J Immunol. 1983 Oct;131(4):1838–1842. [PubMed] [Google Scholar]
  3. Garber S. L., Cohen E. P., Chang I. Persistence of the immunoprotective effects of leukemia X fibroblast hybrid cells toward leukemia in histocompatible mice. Leuk Res. 1984;8(2):255–266. doi: 10.1016/0145-2126(84)90149-8. [DOI] [PubMed] [Google Scholar]
  4. Hamilton J. A., Stanley E. R., Burgess A. W., Shadduck R. K. Stimulation of macrophage plasminogen activator activity by colony-stimulating factors. J Cell Physiol. 1980 Jun;103(3):435–445. doi: 10.1002/jcp.1041030309. [DOI] [PubMed] [Google Scholar]
  5. Ishikawa S., Tachibana T. Secondary in vitro generation of CTL specific for MM antigen by stimulation with somatic tumor hybrid, but not with parental tumor cells. J Immunol. 1983 Oct;131(4):2059–2063. [PubMed] [Google Scholar]
  6. Kim B. S., Liang W., Cohen E. P. Tumor-specific immunity induced by somatic hybrids. I. Lack of relationship between immunogenicity and tumorigenicity of selected hybrids. J Immunol. 1979 Aug;123(2):733–738. [PubMed] [Google Scholar]
  7. Kurland J. I., Pelus L. M., Ralph P., Bockman R. S., Moore M. A. Induction of prostaglandin E synthesis in normal and neoplastic macrophages: role for colony-stimulating factor(s) distinct from effects on myeloid progenitor cell proliferation. Proc Natl Acad Sci U S A. 1979 May;76(5):2326–2330. doi: 10.1073/pnas.76.5.2326. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Maizel A., Sahasrabuddhe C., Mehta S., Morgan J., Lachman L., Ford R. Biochemical separation of a human B cell mitogenic factor. Proc Natl Acad Sci U S A. 1982 Oct;79(19):5998–6002. doi: 10.1073/pnas.79.19.5998. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Meltzer M. S., Benjamin W. R., Farrar J. J. Macrophage activation for tumor cytotoxicity: induction of macrophage tumoricidal activity by lymphokines from EL-4, a continuous T cell line. J Immunol. 1982 Dec;129(6):2802–2807. [PubMed] [Google Scholar]
  10. Moore R. N., Oppenheim J. J., Farrar J. J., Carter C. S., Jr, Waheed A., Shadduck R. K. Production of lymphocyte-activating factor (Interleukin 1) by macrophages activated with colony-stimulating factors. J Immunol. 1980 Sep;125(3):1302–1305. [PubMed] [Google Scholar]
  11. Sidman C. L., Marshall J. D. B cell maturation factor: effects on various cell populations. J Immunol. 1984 Feb;132(2):845–850. [PubMed] [Google Scholar]
  12. Sidman C. L., Paige C. J., Schreier M. H. B cell maturation factor (BMF): a lymphokine or family of lymphokines promoting the maturation of B lymphocytes. J Immunol. 1984 Jan;132(1):209–222. [PubMed] [Google Scholar]
  13. Slomski R., Wang D. R., Cohen E. P. Surface antigens of immunoprotective leukaemia x fibroblast hybrid cells which have lost malignant properties in histocompatible mice differ from the malignant parental cells. Immunology. 1984 Jun;52(2):281–290. [PMC free article] [PubMed] [Google Scholar]
  14. Stanley E. R., Cifone M., Heard P. M., Defendi V. Factors regulating macrophage production and growth: identity of colony-stimulating factor and macrophage growth factor. J Exp Med. 1976 Mar 1;143(3):631–647. doi: 10.1084/jem.143.3.631. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Stanley E. R. Colony-stimulating factor (CSF) radioimmunoassay: detection of a CSF subclass stimulating macrophage production. Proc Natl Acad Sci U S A. 1979 Jun;76(6):2969–2973. doi: 10.1073/pnas.76.6.2969. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Stanley E. R., Guilbert L. J. Methods for the purification, assay, characterization and target cell binding of a colony stimulating factor (CSF-1). J Immunol Methods. 1981;42(3):253–284. doi: 10.1016/0022-1759(81)90156-3. [DOI] [PubMed] [Google Scholar]
  17. Stanley E. R., Heard P. M. Factors regulating macrophage production and growth. Purification and some properties of the colony stimulating factor from medium conditioned by mouse L cells. J Biol Chem. 1977 Jun 25;252(12):4305–4312. [PubMed] [Google Scholar]
  18. Teh H. S., Yu M. Activation of nonspecific killer cells by interleukin 2-containing supernatants. J Immunol. 1983 Oct;131(4):1827–1833. [PubMed] [Google Scholar]
  19. Ting C. C., Hargrove M. E. Activation of natural killer-derived cytotoxic T lymphocytes. I. Regulation by macrophage and prostaglandins. J Immunol. 1983 Oct;131(4):1734–1741. [PubMed] [Google Scholar]
  20. Toffaletti D. L., Darrow T. L., Scott D. W. Augmentation of syngeneic tumor-specific immunity by semiallogeneic cell hybrids. J Immunol. 1983 Jun;130(6):2982–2986. [PubMed] [Google Scholar]
  21. Wang D. R., Slomski R., Cohen E. P. Leukemia X fibroblast hybrid cells prolong the lives of leukemic mice. Eur J Cancer Clin Oncol. 1985 May;21(5):637–643. doi: 10.1016/0277-5379(85)90093-8. [DOI] [PubMed] [Google Scholar]
  22. Watson J. D. Biology and biochemistry of T cell-derived lymphokines. I. The coordinate synthesis of interleukin 2 and colony-stimulating factors in a murine T cell lymphoma. J Immunol. 1983 Jul;131(1):293–297. [PubMed] [Google Scholar]
  23. Welte K., Wang C. Y., Mertelsmann R., Venuta S., Feldman S. P., Moore M. A. Purification of human interleukin 2 to apparent homogeneity and its molecular heterogeneity. J Exp Med. 1982 Aug 1;156(2):454–464. doi: 10.1084/jem.156.2.454. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES