Skip to main content
NCBI home page
Immunology logoLink to Immunology
. 1986 Mar;57(3):461–466.

Transformation induced by Echinococcus granulosus protoscoleces in unprimed murine spleen cells: identity and MHC restriction of participating cell types.

D A Cox, J B Dixon, S Marshall-Clarke
PMCID: PMC1453830  PMID: 3485564

Abstract

Transformation induced by Echinococcus granulosus in unprimed murine spleen cells in vitro was not prevented by rigorous T- or B-cell depletion. When naïve T- or B-cell populations were further depleted of macrophages, B-cell transformation was relatively unaffected whereas T-cell transformation was totally inhibited. Peritoneal macrophages from infected mice could present a mitogenic stimulus to purified, naïve T-cells, provided that the T-cells had identity in the I region of the H2 complex. K/D identity was neither necessary nor sufficient. It is concluded that transformation can be induced by E. granulosus in both T- and B-cells and that, at least in naïve T-cells, the stimulus can be an I region-restricted, macrophage-presented signal.

Full text

PDF
461

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Ali-Khan Z. Pathological changes in the lymphoreticular tissues of Swiss mice infected with Echinococcus granulosus cysts. Z Parasitenkd. 1978 Dec 21;58(1):47–54. doi: 10.1007/BF00930791. [DOI] [PubMed] [Google Scholar]
  2. Allan D., Jenkins P., Connor R. J., Dixon J. B. A study of immunoregulation of BALB/c mice by Echinococcus granulosus equinus during prolonged infection. Parasite Immunol. 1981 Summer;3(2):137–142. doi: 10.1111/j.1365-3024.1981.tb00391.x. [DOI] [PubMed] [Google Scholar]
  3. Bailey M., Lloyd S., Martin S. C., Soulsby E. J. In vitro induction of lymphocyte responsiveness by a Strongylus vulgaris-derived mitogen. Z Parasitenkd. 1984;70(2):229–242. doi: 10.1007/BF00942226. [DOI] [PubMed] [Google Scholar]
  4. Baron R. W., Tanner C. E. Echinococcus multilocularis in the mouse: the in vitro protoscolicidal activity of peritoneal macrophages. Int J Parasitol. 1977 Dec;7(6):489–495. doi: 10.1016/0020-7519(77)90011-x. [DOI] [PubMed] [Google Scholar]
  5. Butterworth A. E., Sturrock R. F., Houba V., Taylor R. Schistosoma mansoni in baboons. Antibody-dependent cell-mediated damage to 51Cr-labelled schistosomula. Clin Exp Immunol. 1976 Jul;25(1):95–102. [PMC free article] [PubMed] [Google Scholar]
  6. Clayton C. E., Sacks D. L., Ogilvie B. M., Askonas B. A. Membrane fractions of trypanosomes mimic the immunosuppressive and mitogenic effects of living parasites on the host. Parasite Immunol. 1979 Autumn;1(3):241–249. doi: 10.1111/j.1365-3024.1979.tb00709.x. [DOI] [PubMed] [Google Scholar]
  7. Dixon J. B., Jenkins P., Allan D. Immune recognition of Echinococcus granulosus. 1. Parasite-activated, primary transformation by normal murine lymph node cells. Parasite Immunol. 1982 Jan;4(1):33–45. doi: 10.1111/j.1365-3024.1982.tb00418.x. [DOI] [PubMed] [Google Scholar]
  8. Doughty B. L., Phillips S. M. Delayed hypersensitivity granuloma formation and modulation around Schistosoma mansoni eggs in vitro. II. Regulatory T cell subsets. J Immunol. 1982 Jan;128(1):37–42. [PubMed] [Google Scholar]
  9. Elliott D. E., Boros D. L. Schistosome egg antigen(s) presentation and regulatory activity by macrophages isolated from vigorous or immunomodulated liver granulomas of Schistosoma mansoni-infected mice. J Immunol. 1984 Mar;132(3):1506–1510. [PubMed] [Google Scholar]
  10. Judson D. G., Dixon J. B., Clarkson M. J., Pritchard J. Ovine hydatidosis: some immunological characteristics of the seronegative host. Parasitology. 1985 Oct;91(Pt 2):349–357. doi: 10.1017/s0031182000057413. [DOI] [PubMed] [Google Scholar]
  11. Judson D. G., Dixon J. B., Skerritt G. C., Stallbaumer M. Mitogenic effect of Coenurus cerebralis cyst fluid. Res Vet Sci. 1984 Jul;37(1):128–128. [PubMed] [Google Scholar]
  12. Krco C. J., Wassom D. L., Abramson E. J., David C. S. Cloned T cells recognize Trichinella spiralis antigen in association with an Ek beta Ek alpha restriction element. Immunogenetics. 1983;18(5):435–444. doi: 10.1007/BF00364385. [DOI] [PubMed] [Google Scholar]
  13. Ly I. A., Mishell R. I. Separation of mouse spleen cells by passage through columns of sephadex G-10. J Immunol Methods. 1974 Aug;5(3):239–247. doi: 10.1016/0022-1759(74)90108-2. [DOI] [PubMed] [Google Scholar]
  14. Osborn P. J., Heath D. D. Immunisation of lambs against Echinococcus granulosus using antigens obtained by incubation of oncospheres in vitro. Res Vet Sci. 1982 Jul;33(1):132–133. [PubMed] [Google Scholar]
  15. Ottesen E. A. Modulation of the host response in human schistosomiasis. I. Adherent suppressor cells that inhibit lymphocyte proliferative responses to parasite antigens. J Immunol. 1979 Oct;123(4):1639–1644. [PubMed] [Google Scholar]
  16. Rickard M. D., Williams J. F. Hydatidosis/cysticercosis: immune mechanisms and immunization against infection. Adv Parasitol. 1982;21:229–296. doi: 10.1016/s0065-308x(08)60277-8. [DOI] [PubMed] [Google Scholar]
  17. Riley E. M., Dixon J. B., Kelly D. F., Cox D. A. The immune response to Echinococcus granulosus: sequential histological observations of lymphoreticular and connective tissues during early murine infection. J Comp Pathol. 1985 Jan;95(1):93–104. doi: 10.1016/0021-9975(85)90081-7. [DOI] [PubMed] [Google Scholar]
  18. Smithers S. R., Terry R. J., Hockley D. J. Host antigens in schistosomiasis. Proc R Soc Lond B Biol Sci. 1969 Feb 25;171(1025):483–494. doi: 10.1098/rspb.1969.0007. [DOI] [PubMed] [Google Scholar]
  19. Varela-Díaz V. M., Williams J. F., Coltorti E. A., Williams C. S. Survival of cysts of Echinococcus granulosus after transplant into homologous and heterologous hosts. J Parasitol. 1974 Aug;60(4):608–612. [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES