Skip to main content
PMC home page
Immunology logoLink to Immunology
. 1986 Apr;57(4):559–565.

Induction of experimental autoimmune uveoretinitis by T-cell lines.

L A Rozenszajn, C Muellenberg-Coulombre, I Gery, M el-Saied, T Kuwabara, M Mochizuki, Z Lando, R B Nussenblatt
PMCID: PMC1453867  PMID: 3485569

Abstract

Experimental autoimmune uveoretinitis was induced in genetically susceptible Lewis rats by passive transfer of T-lymphocyte cell lines from long-term cultures primed against soluble retinal antigen (S-Ag). A continuous T-cell line was established from non-adherent lymph node cells of S-Ag-immunized Lewis rats. The lymphoid cells were propagated in vitro by serially restimulating them with S-Ag in the presence of irradiated syngeneic spleen cells and expanding them in IL-2-containing media. The cell lines exhibited markers specific for T lymphocytes and the majority had the helper phenotype. When naïve rats were inoculated intravenously with anti S-Ag T-cell lines re-exposed to the antigen prior to injection, they developed uveoretinitis with both clinical and histological characteristics in half the time required by S-Ag to induce the disease by active immunization. The rats exhibited a delayed hypersensitivity skin reaction towards S-Ag.

Full text

PDF
559

Images in this article

562Figure 2
on p.562
563Figure 3
on p.563

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BULLINGTON S. J., WAKSMAN B. H. Uveitis in rabbits with experimental allergic encephalomyelitis; results produced by injection of nervous tissue and adjuvants. AMA Arch Ophthalmol. 1958 Mar;59(3):435–445. doi: 10.1001/archopht.1958.00940040141017. [DOI] [PubMed] [Google Scholar]
  2. Ben-Nun A., Cohen I. R. Experimental autoimmune encephalomyelitis (EAE) mediated by T cell lines: process of selection of lines and characterization of the cells. J Immunol. 1982 Jul;129(1):303–308. [PubMed] [Google Scholar]
  3. Ben-Nun A., Lando Z. Detection of autoimmune cells proliferating to myelin basic protein and selection of T cell lines that mediate experimental autoimmune encephalomyelitis (EAE) in mice. J Immunol. 1983 Mar;130(3):1205–1209. [PubMed] [Google Scholar]
  4. Ben-Nun A., Wekerle H., Cohen I. R. The rapid isolation of clonable antigen-specific T lymphocyte lines capable of mediating autoimmune encephalomyelitis. Eur J Immunol. 1981 Mar;11(3):195–199. doi: 10.1002/eji.1830110307. [DOI] [PubMed] [Google Scholar]
  5. Farrar J. J., Benjamin W. R., Hilfiker M. L., Howard M., Farrar W. L., Fuller-Farrar J. The biochemistry, biology, and role of interleukin 2 in the induction of cytotoxic T cell and antibody-forming B cell responses. Immunol Rev. 1982;63:129–166. doi: 10.1111/j.1600-065x.1982.tb00414.x. [DOI] [PubMed] [Google Scholar]
  6. Faure J. P. Autoimmunity and the retina. Curr Top Eye Res. 1980;2:215–302. [PubMed] [Google Scholar]
  7. Gillis S., Smith K. A. Long term culture of tumour-specific cytotoxic T cells. Nature. 1977 Jul 14;268(5616):154–156. doi: 10.1038/268154a0. [DOI] [PubMed] [Google Scholar]
  8. Gregerson D. S., Abrahams I. W. Immunologic and biochemical properties of several retinal proteins bound by antibodies in sera from animals with experimental autoimmune uveitis and uveitis patients. J Immunol. 1983 Jul;131(1):259–264. [PubMed] [Google Scholar]
  9. Gregerson D. S., Abrahams I. W., Thirkill C. E. Serum antibody levels of uveitis patients to bovine retinal antigens. Invest Ophthalmol Vis Sci. 1981 Nov;21(5):669–680. [PubMed] [Google Scholar]
  10. Holoshitz J., Naparstek Y., Ben-Nun A., Marquardt P., Cohen I. R. T lymphocyte lines induce autoimmune encephalomyelitis, delayed hypersensitivity and bystander encephalitis or arthritis. Eur J Immunol. 1984 Aug;14(8):729–734. doi: 10.1002/eji.1830140811. [DOI] [PubMed] [Google Scholar]
  11. Horan P. K., Wheeless L. L., Jr Quantitative single cell analysis and sorting. Science. 1977 Oct 14;198(4313):149–157. doi: 10.1126/science.905822. [DOI] [PubMed] [Google Scholar]
  12. Loken M. R., Herzenber L. A. Analysis of cell populations with a fluorescence-activated cell sorter. Ann N Y Acad Sci. 1975 Jun 30;254:163–171. doi: 10.1111/j.1749-6632.1975.tb29166.x. [DOI] [PubMed] [Google Scholar]
  13. Nussenblatt R. B., Kuwabara T., de Monasterio F. M., Wacker W. B. S-antigen uveitis in primates. A new model for human disease. Arch Ophthalmol. 1981 Jun;99(6):1090–1092. doi: 10.1001/archopht.1981.03930011090021. [DOI] [PubMed] [Google Scholar]
  14. Nussenblatt R. B., Mittal K. K., Ryan S., Green W. R., Maumenee A. E. Birdshot retinochoroidopathy associated with HLA-A29 antigen and immune responsiveness to retinal S-antigen. Am J Ophthalmol. 1982 Aug;94(2):147–158. doi: 10.1016/0002-9394(82)90069-1. [DOI] [PubMed] [Google Scholar]
  15. Nussenblatt R. B., Rodrigues M. M., Salinas-Carmona M. C., Gery I., Cevario S., Wacker W. Modulation of experimental autoimmune uveitis with cyclosporin A. Arch Ophthalmol. 1982 Jul;100(7):1146–1149. doi: 10.1001/archopht.1982.01030040124022. [DOI] [PubMed] [Google Scholar]
  16. Nussenblatt R. B., Shichi H., Kuwabara T., Cevario S., Gery I. Resemblance between rhodopsin kinase and S-antigen induced uveitis. Br J Ophthalmol. 1981 Nov;65(11):778–782. doi: 10.1136/bjo.65.11.778. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Ortiz-Ortiz L., Weigle W. O. Cellular events in the induction of experimental allergic encephalomyelitis in rats. J Exp Med. 1976 Sep 1;144(3):604–616. doi: 10.1084/jem.144.3.604. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Rennard S. I., Berg R., Martin G. R., Foidart J. M., Robey P. G. Enzyme-linked immunoassay (ELISA) for connective tissue components. Anal Biochem. 1980 May 1;104(1):205–214. doi: 10.1016/0003-2697(80)90300-0. [DOI] [PubMed] [Google Scholar]
  19. Ruscetti F. W., Morgan D. A., Gallo R. C. Functional and morphologic characterization of human T cells continuously grown in vitro. J Immunol. 1977 Jul;119(1):131–138. [PubMed] [Google Scholar]
  20. Salinas-Carmona M. C., Nussenblatt R. B., Gery I. Experimental autoimmune uveitis in the athymic nude rat. Eur J Immunol. 1982 Jun;12(6):480–484. doi: 10.1002/eji.1830120606. [DOI] [PubMed] [Google Scholar]
  21. Schreier M. H., Tees R. Clonal induction of helper T cells: conversion of specific signals into nonspecific signals. Int Arch Allergy Appl Immunol. 1980;61(2):227–237. doi: 10.1159/000232437. [DOI] [PubMed] [Google Scholar]
  22. Wacker W. B., Lipton M. M. Experimental allergic uveitis: homologous retina as uveitogenic antigen. Nature. 1965 Apr 17;206(981):253–254. doi: 10.1038/206253a0. [DOI] [PubMed] [Google Scholar]
  23. Watson J. Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J Exp Med. 1979 Dec 1;150(6):1510–1519. doi: 10.1084/jem.150.6.1510. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Immunology are provided here courtesy of British Society for Immunology

RESOURCES